Abstract: A dental floss holder has a casing containing a cartridge that is slidable within said casing through various sequential positions. The cartridge contains a plurality of U-shaped segments with each segment containing a length of dental floss held across a mouth of the segment in a taut position. As the dental floss is used, the cartridge can be advanced to extend a first segment beyond the cartridge and sever the first segment from the cartridge. The severed first segment is then discarded and the dental floss in the next segment is used. The method is repeated until all of the segments of the cartridge have been used and discarded and a new cartridge is then inserted into the casing.