Abstract: Aspects of the disclosure relate to bioreactors for maintaining biological objects (e.g., organ or engineered tissues) under culture conditions. In some embodiments, bioreactors and cover assemblies are provided that comprise a support base for supporting a biological object. In some embodiments, bioreactors and cover assemblies are provided that comprise a movable support base configured such that a user can manipulate its position and thus the relative position of the biological object.

Abstract: Aspects of the disclosure relate to rotating bioreactors and articles and methods that are useful for adapting a rotating bioreactor for use with tissues or scaffolds of different sizes. In some embodiments, bioreactors comprising a reservoir and an arbor assembly are provided herein, in which the arbor assembly comprises a rotatable support to which a tissue or tissue scaffold can be attached.

Abstract: Aspects of the invention relate to a combination of techniques and/or materials that can be used to form a synthetic scaffold for solid and/or hollow organs or tissue. In some embodiments, methods are provided that involve assembling a synthetic scaffold using a first material for a first structural component and a second material for a second structural component, in which the first or second structural component is a perfusion pathway. In some embodiments, materials (e.g., synthetic materials) for the scaffold are printed, molded, cast, polymerized, or electrospun. In some embodiments, a scaffold may mimic a natural scaffold or several features of a natural scaffold.

Abstract: Aspects of the disclosure provide techniques for detecting differences and/or changes in biological and non-biological material using infrared imaging. Aspects of the disclosure are useful for monitoring and evaluating synthetic scaffolds and engineered tissue and organs for tissue engineering and transplantation.

Abstract: A synthetic scaffold for replacing at least a portion of an airway includes an airway mold, one or more structural ribs on the airway mold, and a non-structural wall. Each of the one or more structural ribs is formed from a first material and the non-structural wall is formed from a second material. The non-structural wall coats the airway mold and forms a conduit that incorporates the one or more structural ribs.

Abstract: According to some aspects, tissue scaffolds are provided that comprise one or more types of nanofibers. In some embodiments, one or more design features are incorporated into a tissue scaffold (e.g.

Abstract: The invention relates to a bioreactor for charging the outside and the interior of a hollow element (1) or hollow element framework with a liquid, having a housing (2) accommodating the liquid, forming a liquid surface, and a rotation device (3) arranged within the housing (2) and receiving the hollow element (1), which rotation device (3) is for rotating the hollow element (1) about the longitudinal axis (4) thereof in the region of the liquid surface. In known bioreactors of this type, the interior of the hollow element must be flushed with a special device, and so here also a liquid exchange takes place.

Abstract: Aspects of the disclosure relate to synthetic tissue or organ scaffolds and methods and compositions for promoting or maintaining their structural integrity. Aspects of the disclosure are useful to prevent scaffold damage (e.g., delamination) during or after implantation into a host. Aspects of the disclosure are useful to stabilize tissue or organ scaffolds that include electrospun fibers.