Abstract: Disclosed is a method for capturing target cells or molecules in solution, comprising steps of: (I) getting medium containing said target cells or molecules into an apparatus comprising a capturing device for capturing said target cells or molecules; (II) getting said medium flow through said capturing device; (III) removing unbound debris, cells and molecules; (IV) getting said target cells or molecules detached from the capturing device; and (V) collecting said target cells or molecules; wherein said capturing device comprises at least one functionalized mesh comprising a mesh substrate and a functional layer formed on said mesh substrate, wherein said functional layer comprises capturing substances that can specifically bind with said target cells or molecules. The method has high specificity, as well as high throughput, and is suitable for capturing cells or molecules in a solution or expressed at the surface of cell membranes. It is particularly suited to capture and sort circulating tumor cells.