Abstract: The present disclosure provides compositions, systems, and tools for modeling liver inflammation and methods of using the same. The disclosure provides micropatterned hepatocyte co-cultures where individual cell populations remain functionally stable during long-term culture. The in vitro liver inflammation models of the present disclosure may be useful for evaluating inflammation-mediated toxicities of compounds in a pre-clinical setting.

Abstract: Systems and methods for using stamps to print or mask materials on a substrate service. In one particular embodiment, the systems and methods include a microcontacting stamp that has a plurality of rigid posts each having a resilient pad at its distal end. Each post is fitted within an aperture located in a guide plate such that the post may move longitudinally within the guide plate. The guide plate includes a variety of apertures that typically are aligned with the wells of a microtiter plate. The apertures extend typically through the entire thickness of the guide plate. On one side of the guide plate is a resilient member that extends over one or more of the apertures thereby holding the post in place.

Abstract: Systems and methods for using stamps to print or mask materials on a substrate service. In one particular embodiment, the systems and methods include a microcontacting stamp that has a plurality of rigid posts each having a resilient pad at its distal end. Each post is fitted within an aperture located in a guide plate such that the post may move longitudinally within the guide plate. The guide plate includes a variety of apertures that typically are aligned with the wells of a microtiter plate. The apertures extend typically through the entire thickness of the guide plate. On one side of the guide plate is a resilient member that extends over one or more of the apertures thereby holding the post in place.