Abstract: A resuspension system includes a flexible media bag removably disposed within a rigid support container. The media bag includes an inlet port, an outlet port, and an assembly for mixing and resuspending fluids within the media bag. The assembly for mixing and resuspending includes a peristaltic pump which withdraws fluid from the media bag and introduces the fluid back into the media bag through a mixing block. The mixing block includes a housing having a conical top surface that extends from a hose barb to a circular sidewall. An inlet passageway vertically extends through the hose barb into the housing. A plurality of curved outlet channels extend from the inlet passageway through the circular sidewall of the housing. The outlet channels exit at such an orientation that as fluid passes through the outlet channels, a vortex flow pattern is created within the media bag.