Abstract: The present invention relates to a process of fermenting a strain of Phellinus linteus fungus, in which the fungus is incubated in a medium under the condition for the best yield control with the aim to obtain the best yield of mycelia and polysaccharides, where the best ratio of the medium having potato extract and glucose contains 200 g of potato extract and 20 g glucose per liter. The fungus is inoculated to the medium and can generate the best yield of mycelia and polysaccharides under the condition for the best yield control. Moreover, the co-fermentation of the Phellinus linteus fungus with a single- or multiple-component formulation of Chinese herbal medicines (more than two different kinds of Chinese herbal medicine) is shown to generate a better yield of mycelia and polysaccharides as well as an increase in the anti-tumor activity, compared to the fermentation of the Phellinus linteus fungus alone.