Abstract: A method segments iris images from eye image data captured from non-cooperative subjects. The method includes receiving a frame of eye image data, and determining whether a pupil exists in the image by detecting glare areas in the image. Upon finding a pupil, subsequent images are processed with reference to the pupil location and a radius is calculated for the pupil. A k means clustering method and principal component analysis are used to locate pupil boundary points, which are fitted to a conic. Using the pupil boundary, an angular derivative is computed for each frame having a pupil and iris boundary points are fitted to a conic to identify an iris region between the iris boundary and the pupil boundary. Noise data are then removed from the iris region to generate an iris segment. A method for evaluating iris frame quality and iris image segmentation quality is also disclosed.