Abstract: The present invention relates to a method of enriching a pathogen using an homobifunctional imidoester group, and the method of enriching a pathogen and extracting nucleic acids using homobifunctional imidoester compound (DMA, DMP, DMS) according to the present invention can quickly extract a small amount of pathogen contained in a sample without the use of special equipment and can be used as in situ diagnosis, and since it is possible to enrich a pathogen and extract nucleic acids simultaneously in one tube or a chip, it has the advantage of being more efficient than the conventional method, saving time and cost, and being easy to use.

Abstract: The present invention relates to a method for lysing pathogen lysis and a method for extracting nucleic acid using zinc oxide nanostar, and the method for extracting nucleic acid using zinc oxide nanostar according to the present invention can extract nucleic acids by lysing cells of a pathogen without using a lysis buffer and can extract nucleic acid of high-purity and high-concentration by preventing nucleic acid degradation and fragmentation through various substances including salts contained in the lysis buffer at high concentration. In addition, the zinc oxide nanostar of the present invention (200 to 900 nm) has superior cell lysis capacity compared to the conventional zinc oxide nanoparticles (20 to 50 nm), thereby increasing the nucleic acid extraction efficiency and can extract at room temperature without a heating step to use as a field diagnostic method.

Abstract: A method for concentrating microorganisms, includes modifying an object by introducing an amine group into the object (step 1); and contacting a sample including a microorganism and dimethyl 3,3?-dithiobispropionimidate (DTBP) each other on the modified object (step 2), wherein the object is any one of a thin film device, a magnetic bead, a ring resonator, and a nanoparticle.

Abstract: A method for concentrating microorganisms, includes modifying an object by introducing an amine group into the object (step 1); and contacting a sample including a microorganism and dimethyl 3,3?-dithiobispropionimidate (DTBP) each other on the modified object (step 2), wherein the object is any one of a thin film device, a magnetic bead, a ring resonator, and a nanoparticle.

Abstract: The present invention relates to a method of enriching pathogens and extracting nucleic acids using diatomaceous earth functionalized with amines and dimethyl suberimidate as a crosslinking agent, wherein negatively charged pathogens are electrically absorbed on the surface of positively charged diatomaceous earth by the crosslinking agent, and nucleic acid released after cell lysis can be immobilized and separated on the diatomaceous earth through reversible crosslinking with amine groups of diatomaceous earth. The above method can be used as a point-of-care testing because it is possible to concentrate pathogens and extract nucleic acids at the same time as an all-in-one device without the use of special equipment and electricity (electric power), and it is superior in time and cost savings than the conventional method, and the extracted nucleic acid has an advantage that can be usefully used for diagnosis and treatment of the diseases.

Abstract: The present invention relates to a method of enriching a pathogen using an homobifunctional imidoester group, and the method of enriching a pathogen and extracting nucleic acids using homobifunctional imidoester compound (DMA, DMP, DMS) according to the present invention can quickly extract a small amount of pathogen contained in a sample without the use of special equipment and can be used as in situ diagnosis, and since it is possible to enrich a pathogen and extract nucleic acids simultaneously in one tube or a chip, it has the advantage of being more efficient than the conventional method, saving time and cost, and being easy to use.

Abstract: The present invention relates to a method of concentrating microorganisms and a method of extracting nucleic acids using diatomaceous earth and developed a technology capable of concentrating microorganisms about 4-8 times using the modified diatomaceous earth and directly extracting nucleic acids from the concentrated microorganism using dimethyl adipimidate (DMA). It is expected to significantly reduce external contamination, cost, time and complexity by enabling microbial concentration and nucleic acid extraction techniques in one tube.

Abstract: The present invention relates to a ribonucleic acid (RNA) extraction method using a solid subject, the method including activating a subject with a reactive amine group, injecting a sample having 1×101 to 1×103 cells/ml and a dimethyl suberimidate (DMS) compound or a dimethyl pimelimidate (DMP) compound into the subject, and forming a complex having the RNA within the sample and the compound, and extracting the RNA by treating elution buffer to the subject on which the complex is formed. The subject, particularly, the thin film device used for extracting the RNA, has improved hydrophilicity compared to the conventional silicon substrate, so that RNA is extracted more efficiently.