Patents Assigned to Inscripta, Inc.
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 11046928
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: November 23, 2020
    Date of Patent: June 29, 2021
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 11034953
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: March 2, 2021
    Date of Patent: June 15, 2021
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Kevin Ness, Phillip Belgrader, Don Masquelier, Ryan Gill
  • Simultaneous multiplex genome editing in yeast
    Patent number: 11034945
    Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.
    Type: Grant
    Filed: September 30, 2020
    Date of Patent: June 15, 2021
    Assignee: Inscripta, Inc.
    Inventors: Miles Gander, Tian Tian, Skylar Stefani
  • Flow through electroporation modules and instrumentation
    Patent number: 11015162
    Abstract: The present disclosure provides a flow-through electroporation device configured for use in an automated multi-module cell processing environment and configured to decrease cell processing time and the risk of clogging.
    Type: Grant
    Filed: January 2, 2021
    Date of Patent: May 25, 2021
    Assignee: Inscripta, Inc.
    Inventors: Nathan Bade, Jorge Bernate, Phillip Belgrader, Don Masquelier
  • Cascade/dCas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells
    Patent number: 11008557
    Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.
    Type: Grant
    Filed: December 15, 2020
    Date of Patent: May 18, 2021
    Assignee: Inscripta, Inc.
    Inventors: Aamir Mir, Andrew Garst, Stephen Federowicz, Kyle Seamon
  • Simultaneous multiplex genome editing in yeast
    Patent number: 11001831
    Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.
    Type: Grant
    Filed: October 1, 2020
    Date of Patent: May 11, 2021
    Assignee: Inscripta, Inc.
    Inventors: Miles Gander, Tian Tian, Skylar Stefani
  • Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
    Patent number: 10995424
    Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.
    Type: Grant
    Filed: November 17, 2020
    Date of Patent: May 4, 2021
    Assignee: Inscripta, Inc.
    Inventors: Richard Fox, Daniel Held
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10954485
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: November 23, 2020
    Date of Patent: March 23, 2021
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10954512
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: December 22, 2020
    Date of Patent: March 23, 2021
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Kevin Ness, Phillip Belgrader, Don Masquelier, Ryan Gill
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10947532
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: May 14, 2019
    Date of Patent: March 16, 2021
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Increased nucleic-acid guided cell editing in yeast
    Patent number: 10927385
    Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.
    Type: Grant
    Filed: June 17, 2020
    Date of Patent: February 23, 2021
    Assignee: Inscripta, Inc.
    Inventors: Krishna Kannan, Miles Gander, Eileen Spindler, Paul Hardenbol
  • Genome-wide rationally-designed mutations leading to enhanced lysine production in
    Patent number: 10920189
    Abstract: The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.
    Type: Grant
    Filed: June 18, 2020
    Date of Patent: February 16, 2021
    Assignee: Inscripta, Inc.
    Inventors: Richard Fox, Daniel Held, Eric Abbate, Michael Clay, Katherine Krouse, Nandini Krishnamurthy, Krishna Yerramsetty
  • Flow through electroporation modules and instrumentation
    Patent number: 10907125
    Abstract: The present disclosure provides a flow-through electroporation device configured for use in an automated multi-module cell processing environment and configured to decrease cell processing time and the risk of clogging.
    Type: Grant
    Filed: June 17, 2020
    Date of Patent: February 2, 2021
    Assignee: Inscripta, Inc.
    Inventors: Nathan Bade, Jorge Bernate, Phillip Belgrader, Don Masquelier
  • Automated cell processing methods, modules, instruments, and systems comprising flow-through electroporation devices
    Patent number: 10907178
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments comprising one or more flow-through electroporation devices or modules are provided to automate genome editing in live cells.
    Type: Grant
    Filed: May 30, 2019
    Date of Patent: February 2, 2021
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader, Kevin Ness
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10894958
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: August 9, 2020
    Date of Patent: January 19, 2021
    Assignee: Inscripta, Inc.
    Inventors: Kevin Ness, Phillip Belgrader, Don Masquelier, Jorge Bernate, Ryan Gill
  • Mad nucleases
    Patent number: 10883095
    Abstract: The present disclosure provides new RNA-guided nucleases for making rational, direct edits to nucleic acids in live cells.
    Type: Grant
    Filed: April 1, 2020
    Date of Patent: January 5, 2021
    Assignee: Inscripta, Inc.
    Inventors: Benjamin Mijts, Juhan Kim, Aamir Mir, Andrew Garst, Kyle Seamon
  • Methods for controlling the growth of prokaryotic and eukaryotic cells
    Patent number: 10883077
    Abstract: The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.
    Type: Grant
    Filed: March 31, 2020
    Date of Patent: January 5, 2021
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier
  • Engineered enzymes
    Patent number: 10876102
    Abstract: The present disclosure provides engineered RNA-guided enzymes for editing live cells.
    Type: Grant
    Filed: April 9, 2020
    Date of Patent: December 29, 2020
    Assignee: Inscripta, Inc.
    Inventors: Benjamin Mijts, Juhan Kim, Aamir Mir, Kyle Seamon, Andrew Garst
  • Nucleic acid-guided nucleases
    Patent number: 10870841
    Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.
    Type: Grant
    Filed: August 9, 2020
    Date of Patent: December 22, 2020
    Assignee: Inscripta, Inc.
    Inventors: Benjamin Mijts, Aamir Mir, Andrew Garst, Juhan Kim, Kyle Seamon
  • Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
    Patent number: 10858761
    Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.
    Type: Grant
    Filed: February 22, 2020
    Date of Patent: December 8, 2020
    Assignee: Inscripta, Inc.
    Inventors: Richard Fox, Daniel Held