Abstract: A method of opportunistic access to a frequency band dedicated to a synchronous primary network by a secondary user. The frequency band is divided into K independent channels to which an indicator Bk and an average availability Sk are assigned. At each time frame t, the method includes observing, by the secondary user, the channel i having the greatest indicator Bi(t); and if the channel i is busy, updating the average availability Si of channel i and updating indicator Bi (t) of the channel as a function of Si, and if the channel i is free: updating the average availability Si of channel i, transmitting data set up during the frame t on channel i, computing a quality index Ri (t) of channel i, and updating indicators Bk (t) of the K channels as a function of the average availabilities Sk and Ri (t).

Abstract: The invention relates to an isolated polypeptide with an glycosyl transferase enzymatic activity for producing dextrans with .alpha.(1.fwdarw.2) sidechains, comprising at least one region for bonding to glucan and a catalytically active region situated beyond the region bonding to glucan. The invention further relates to polynucleotides coding for said enzymes and vectors containing the same.

Abstract: The invention relates to an isolated polypeptide with an glycosyl transferase enzymatic activity for producing dextrans with .alpha.(1.fwdarw.2) sidechains, comprising at least one region for bonding to glucan and a catalytically active region situated beyond the region bonding to glucan. The invention further relates to polynucleotides coding for said enzymes and vectors containing the same.

Abstract: The present invention relates, inter alia, to recombinant Gram-positive Clostridia host cells for producing solvents, fuels and/or chemical intermediates, and preferably ethanol, from plant cell walls comprising: (a) at least one nucleic acid encoding a plant cell wall degrading enzyme, wherein the host cells produce and secrete the plant cell wall degrading enzyme, (b) at least one nucleic acid encoding an enzyme that converts pyruvate to acetaldehyde and at least one nucleic acid encoding an enzyme that converts acetaldehyde to ethanol wherein the host cell is capable of expressing said nucleic acid, and, (c) a mutation in at least one nucleic acid encoding for an enzyme in a metabolic pathway which produces a metabolite other than acetaldehyde from pyruvate or ethanol from acetaldehyde, such that the mutation results in a reduced production of the metabolite.

Abstract: Described herein are molecules, constructs and methods for the production and secretion of polypeptides of interest by host cells, preferably bacterial host cells, and more particularly gram positive bacteria. In particular, the present invention is related to a polynucleic acid encoding a fusion protein and to uses thereof for the secretion of heterologous or homologous polypeptides of interest by a bacterial host cell, preferably Clostridium bacteria. The present invention further relates to methods and constructs for the production and secretion of heterologous or homologous polypeptides of interest proteins by host cells using such polynucleic acids and fusion proteins.

Abstract: The invention relates to applications of the cellulase Cel5H of Saccharophagus degradans and its homologues, functional fragments and/or variants and engineered forms thereof, in the context of recombinant, more particularly solventogenic microorganisms, more particularly C. acetobutylicum. The invention also characterizes a novel domain of the Cel5H cellulase with a putative cellulose-binding module function, and its uses in chimeric proteins for depolymerization of cellulose containing substrates.

Abstract: The invention relates to an isolated polypeptide with an glycosyl transferase enzymatic activity for producing dextrans with .alpha.(1.fwdarw.2) sidechains, comprising at least one region for bonding to glucan and a catalytically active region situated beyond the region bonding to glucan. The invention further relates to polynucleotides coding for said enzymes and vectors containing the same.

Abstract: The present invention relates, inter alia, to recombinant Gram-positive Clostridia host cells for producing solvents, fuels and/or chemical intermediates, and preferably ethanol, from plant cell walls comprising: (a) at least one nucleic acid encoding a plant cell wall degrading enzyme, wherein the host cells produce and secrete the plant cell wall degrading enzyme, (b) at least one nucleic acid encoding an enzyme that converts pyruvate to acetaldehyde and at least one nucleic acid encoding an enzyme that converts acetaldehyde to ethanol wherein the host cell is capable of expressing said nucleic acid, and, (c) a mutation in at least one nucleic acid encoding for an enzyme in a metabolic pathway which produces a metabolite other than acetaldehyde from pyruvate or ethanol from acetaldehyde, such that the mutation results in a reduced production of the metabolite.

Abstract: The invention relates to applications of the cellulase Cel5H of Saccharophagus degradans and its homologues, functional fragments and/or variants and engineered forms thereof, in the context of recombinant, more particularly solventogenic microorganisms, more particularly C. acetobutylicum. The invention also characterizes a novel domain of the Cel5H cellulase with a putative cellulose-binding module function, and its uses in chimeric proteins for depolymerization of cellulose containing substrates.

Abstract: The invention relates to applications of a cellulase of Pseudomonas sp. ND137, functional fragments and/or variants and engineered forms thereof, in the context of industrial bioprocessing, more particularly solvent production.

Abstract: The present invention relates, inter alia, to recombinant Gram-positive Clostridia host cells for producing solvents, fuels and/or chemical intermediates, and preferably ethanol, from plant cell walls comprising: (a) at least one nucleic acid encoding a plant cell wall degrading enzyme, wherein the host cells produce and secrete the plant cell wall degrading enzyme, (b) at least one nucleic acid encoding an enzyme that converts pyruvate to acetaldehyde and at least one nucleic acid encoding an enzyme that converts acetaldehyde to ethanol wherein the host cell is capable of expressing said nucleic acid, and, (c) a mutation in at least one nucleic acid encoding for an enzyme in a metabolic pathway which produces a metabolite other than acetaldehyde from pyruvate or ethanol from acetaldehyde, such that the mutation results in a reduced production of the metabolite.

Abstract: The object of the invention is a process for molding an optical element such as a lens (2). According to the invention, the element is obtained by a reaction injection molding process comprising the steps of mixing at least two polymerizing reactants and forcing the obtained reacting mixture by an injection duct (5) for filling under pressure a scaled mold cavity (4) having an entry side (43) provided with a casting opening (42) and an evacuation side (44) and providing a laminated flow of mixture in a spout (6) having a progressive enlarging transversal section between the outlet opening (52) of the injection duct (5) and the casting opening (42) of the mold cavity (4), for avoiding any turbulent area in said laminated flow, said evacuation side (44) being provided with means (45) for evacuating air contained in the mixture during mold filling. The invention also covers the molding installation adapted for using such a process.

Abstract: The invention relates to an isolated polypeptide with an glycosyl transferase enzymatic activity for producing dextrans with ?(1?2) sidechains, comprising at least one region for bonding to glucan and a catalytically active region situated beyond the region bonding to glucan. The invention further relates to polynucleotides coding for said enzymes and vectors containing the same.

Abstract: The object of the invention is a process for molding an optical element such as a lens (2). According to the invention, the element is obtained by a reaction injection molding process comprising the steps of mixing at least two polymerizing reactants and forcing the obtained reacting mixture by an injection duct (5) for filling under pressure a scaled mold cavity (4) having an entry side (43) provided with a casting opening (42) and an evacuation side (44) and providing a laminated flow of mixture in a spout (6) having a progressive enlarging transversal section between the outlet opening (52) of the injection duct (5) and the casting opening (42) of the mold cavity (4), for avoiding any turbulent area in said laminated flow, said evacuation side (44) being provided with means (45) for evacuating air contained in the mixture during mold filling. The invention also covers the molding installation adapted for using such a process.