Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.
Type:
Grant
Filed:
June 10, 2013
Date of Patent:
October 4, 2016
Assignee:
JOULE UNLIMITED TECHNOLOGIES, INC.
Inventors:
Nikos Basil Reppas, Christian Perry Ridley
Abstract: The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.
Type:
Grant
Filed:
September 8, 2015
Date of Patent:
March 15, 2016
Assignee:
Joule Unlimited Technologies, Inc.
Inventors:
Brian D. Green, Nikos Basil Reppas, Dan Eric Robertson
Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.
Type:
Grant
Filed:
January 9, 2015
Date of Patent:
January 26, 2016
Assignee:
Joule Unlimited Technologies, Inc.
Inventors:
Nikos Basil Reppas, Christian Perry Ridley
Abstract: The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.
Type:
Grant
Filed:
February 11, 2015
Date of Patent:
October 20, 2015
Assignee:
Joule Unlimited Technologies, Inc.
Inventors:
Brian D. Green, Nikos Basil Reppas, Dan Eric Robertson
Abstract: The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.
Type:
Grant
Filed:
January 12, 2012
Date of Patent:
October 6, 2015
Assignee:
Joule Unlimited Technologies, Inc.
Inventors:
David Arthur Berry, Dan Eric Robertson, Frank Anthony Skraly, Brian D. Green, Christian Perry Ridley, Sriram Kosuri, Nikos Basil Reppas, Martha Sholl, Noubar Boghos Afeyan
Abstract: Various 1-alkenes, including 1-nonadecene and 1-octadecene, are synthesized by the engineered microorganisms and methods of the invention. In certain embodiments, the microorganisms comprise recombinant 1-alkene synthases. The engineered microorganisms may be photosynthetic microorganisms such as cyanobacteria.
Type:
Grant
Filed:
June 22, 2010
Date of Patent:
July 7, 2015
Assignee:
Joule Unlimited Technologies, Inc.
Inventors:
Christian Perry Ridley, Nikos Basil Reppas
Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce alkanes, and in particular the use of such organisms for the commercial production of alkanes and related molecules. Other materials, methods, and compositions are also described.
Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert inorganic carbon and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.
Type:
Grant
Filed:
March 23, 2012
Date of Patent:
May 12, 2015
Assignee:
Joule Unlimited Technologies, Inc.
Inventors:
Dan Eric Robertson, Christian Perry Ridley, Nikos Basil Reppas, Jacob Carter Harrison, Michael Connor, Frank Anthony Skraly
Abstract: The invention provides methods and compositions for inhibiting proliferation of a modified host cell outside of a designated process condition. Compositions and methods for providing a host cell having reduced viability when exposed to natural conditions external to a controlled environment are disclosed.
Type:
Grant
Filed:
September 21, 2011
Date of Patent:
April 14, 2015
Assignee:
Joule Unlimited Technologies, Inc.
Inventors:
George Church, Frank A. Skraly, Brian D. Green, Jacob C. Harrison
Abstract: The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.
Type:
Grant
Filed:
May 6, 2013
Date of Patent:
March 24, 2015
Assignee:
Joule Unlimited Technologies, Inc.
Inventors:
Brian D. Green, Nikos Basil Reppas, Dan Eric Robertson
Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.
Type:
Grant
Filed:
April 9, 2012
Date of Patent:
January 13, 2015
Assignee:
Joule Unlimited Technologies, Inc.
Inventors:
Nikos Basil Reppas, Christian Perry Ridley
Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.
Type:
Grant
Filed:
February 12, 2013
Date of Patent:
December 9, 2014
Assignee:
Joule Unlimited Technologies, Inc.
Inventors:
David Arthur Berry, Noubar Boghos Afeyan, Frank Anthony Skraly, Christian Perry Ridley, Dan Eric Robertson, Regina Wilpiszeski, Martha Sholl
Abstract: The present invention provides photobioreactors, solar energy gathering systems, and methods for thermal control of a culture medium containing a phototrophic organism in a photobioreactor, that allow temperature control in a cost effective manner, reducing the energy required for temperature control of a culture medium containing phototrophic microorganisms in a photobioreactor.
Type:
Application
Filed:
April 4, 2014
Publication date:
November 6, 2014
Applicant:
JOULE UNLIMITED TECHNOLOGIES, INC.
Inventors:
Frederick M. Morgan, Stuart A. Jacobson, Johan van Walsem
Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce alkanes, and in particular the use of such organisms for the commercial production of alkanes and related molecules. Other materials, methods, and compositions are also described.
Abstract: Various 1-alkenes, including 1-nonadecene and 1-octadecene, are synthesized by the engineered microorganisms and methods of the invention. In certain embodiments, the microorganisms comprise a recombinant alpha-olefin-associated enzyme. This enzyme may be expressed in combination with a recombinant alkene synthase pathway-related gene. The engineered microorganisms may be photosynthetic microorganisms such as cyanobacteria.
Type:
Application
Filed:
August 22, 2012
Publication date:
July 3, 2014
Applicant:
Joule Unlimited Technologies, Inc.
Inventors:
Nikos Basil Reppas, Christian Perry Ridley, Amy Dearborn
Abstract: The invention relates to a photobioreactor system for a phototrophic microorganism, and culture medium therefor, comprising a reactor chamber and a thermal system.
Type:
Application
Filed:
September 13, 2013
Publication date:
April 10, 2014
Applicant:
Joule Unlimited Technologies, Inc.
Inventors:
Michael Y. Leshchiner, Stuart A. Jacobson
Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into hydrocarbons, e.g., n-alkanes and n-alkenes, wherein the n-alkanes are secreted into the culture medium via recombinantly expressed transporter proteins. In particular, the use of such organisms for the commercial production of n-alkanes and related molecules is contemplated.
Type:
Application
Filed:
October 17, 2013
Publication date:
April 3, 2014
Applicant:
JOULE UNLIMITED TECHNOLOGIES, INC.
Inventors:
Nikos B. Reppas, Kevin Smith, Carolyn Lawrence
Abstract: The NonA alkene synthase in Synechococcus sp. displays selective synthesis of 1-nonadecene. Heterologous recombination of a domain, i.e. the acyl binding domain, with other acyl binding proteins, affects acyl substrate chain-length binding selectivity and therefore the chain-length of the synthesized 1-alkenes. Compositions and methods are provided to selectively synthesize 1-alkenes of various chain lengths.
Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.
Type:
Application
Filed:
June 10, 2013
Publication date:
January 2, 2014
Applicant:
JOULE UNLIMITED TECHNOLOGIES, INC.
Inventors:
Nikos Basil Reppas, Christian Perry Ridley
Abstract: The NonA alkene synthase in Synechococcus sp. displays selective synthesis of 1-nonadecene. Heterologous recombination of a domain, i.e. the acyl binding domain, with other acyl binding proteins, affects acyl substrate chain-length binding selectivity and therefore the chain-length of the synthesized 1-alkenes. Compositions and methods are provided to selectively synthesize 1-alkenes of various chain lengths.