Patents Assigned to Keck Graduate Institute
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Patent number: 11905552Abstract: A digital biosensor for assaying a target nucleic acid and methods of using the biosensor for digitally detecting the target nucleic acid are disclosed wherein the biosensor includes a substrate having a substrate surface and at least two electrodes, a linker molecule having a first moiety conjugated to the substrate surface, a ribonucleoprotein (RNP) conjugated to a second moiety of the linker molecule, and a guide ribonucleic acid (gRNA) having a first sequence capable of binding to the inactive RNP and a second sequence capable of binding to the target nucleic acid.Type: GrantFiled: August 6, 2018Date of Patent: February 20, 2024Assignees: KECK GRADUATE INSTITUTE OF APPLIED LIFE SCIENCES, THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Kiana Aran, Tara deBoer, Irina Conboy
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Patent number: 11447560Abstract: Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.Type: GrantFiled: March 11, 2019Date of Patent: September 20, 2022Assignees: KECK GRADUATE INSTITUTE, H. LUNDBECK A/SInventors: James M. Cregg, John A. Latham, Mark Litton, Randall Schatzman, Ilya I. Tolstrorukov
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Patent number: 11434461Abstract: An airlift bioreactor for perfusion cell culture comprises a single- or multi-tiered riser for working volume scale-up, an internal inclined gravity settler for cell retention, an outlet port for cell-free spent culture medium removal, and an inlet port for fresh medium addition.Type: GrantFiled: March 20, 2019Date of Patent: September 6, 2022Assignee: KECK GRADUATE INSTITUTE OF APPLIED LIFE SCIENCESInventor: Corinna Eleni Doris
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Patent number: 10619164Abstract: In accordance with the invention, isolated nucleic acids, expression methods, host cells, expression vectors, and DNA constructs for producing proteins, and proteins produced using the expression methods are described. More particularly, nucleic acids isolated from Pichia pastoris wherein the nucleic acids have promoter activity are described. The invention also relates to expression methods, host cells, expression vectors, and DNA constructs, for using the Pichia pastoris promoters to produce proteins, and to the proteins produced using the expression methods.Type: GrantFiled: March 7, 2014Date of Patent: April 14, 2020Assignee: Keck Graduate Institute of Applied Life SciencesInventors: Ilya I. Tolstorukov, James M. Cregg
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Patent number: 10428301Abstract: Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency.Type: GrantFiled: October 20, 2016Date of Patent: October 1, 2019Assignee: Keck Graduate InstituteInventors: Robert Doebler, Ali Nadim, James D. Sterling, Anna Hickerson, Barbara Erwin, Denice Woyski, Ryan P. Talbot, Bruce Irvine
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Patent number: 10369119Abstract: Compositions and methods including Bithionol are provided for treating, inhibiting, or preventing caspase-dependent pathogenic agents in a host cell or infected subject. Examples of caspase-dependent pathogenic agents include ricin, anthrax toxin, Botulinum neurotoxin A, diphtheria toxin, Pseudomonas aeruginosa exotoxin A, cholera toxin, Zika virus, and combinations thereof. Compositions and methods also include Bithionol in combination with an antibiotic for more effective clearance of the pathogen and/or toxins.Type: GrantFiled: October 21, 2016Date of Patent: August 6, 2019Assignee: Keck Graduate Institute of Applied Life SciencesInventors: Mikhail Martchenko, William Leonardi
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Patent number: 10259883Abstract: Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.Type: GrantFiled: September 14, 2015Date of Patent: April 16, 2019Assignees: Keck Graduate Institute, Alderbio Holdings LLCInventors: James M. Cregg, John A. Latham, Mark Litton, Randall Schatzman, Ilya I. Tolstrorukov
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Patent number: 10155819Abstract: Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.Type: GrantFiled: January 28, 2014Date of Patent: December 18, 2018Assignees: ALDERBIO HOLDINGS LLC, KECK GRADUATE INSTITUTEInventors: James M Cregg, John Latham, Mark Litton, Randal Schatzman, Ilya Tolstrorukov
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Patent number: 10039758Abstract: Compositions and methods including Amodiaquine (AQ) or N-Desethy Amodiaquine (DEAQ) are provided for treating, inhibiting, or preventing cathepsin B dependent pathogens and toxins in a host cell or infected subject. Compositions and methods also include AQ or DEAQ in combination with an antibiotic for more effective clearance of the pathogen and/or toxins.Type: GrantFiled: October 23, 2015Date of Patent: August 7, 2018Assignee: Keck Graduate Institute of Applied Life SciencesInventors: Mikhail Martchenko, Leeor Zilbermintz, William Leonardi
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Patent number: 9873746Abstract: Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including Pichia. In a preferred embodiment, a first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.Type: GrantFiled: March 24, 2011Date of Patent: January 23, 2018Assignees: KECK GRADUATE INSTITUTE, ALDERBIO HOLDINGS LLCInventors: James M. Cregg, John Latham, Mark Litton, Randall Schatzman, Ilya Tolstrorukov
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Patent number: 9797006Abstract: A disposable and inexpensive biological diagnostic cartridge for the amplification and detection of nucleic acids includes a configuration having a reaction pouch for amplification which is compressed by a flexible pump pouch for detection of the amplified reaction.Type: GrantFiled: April 10, 2013Date of Patent: October 24, 2017Assignee: Keck Graduate Institute of Applied Life SciencesInventors: Angelika Niemz, Anna Hickerson, Kristina Roskos, Hsiang-Wei Lu
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Publication number: 20160297887Abstract: Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.Type: ApplicationFiled: January 28, 2014Publication date: October 13, 2016Applicants: ALDER BIOPHARMACEUTICALS, INC., KECK GRADUATE INSTITUTEInventors: James M. CREGG, John Latham, Mark Litton, Randal Schatzman, Ilya Tolstrorukov
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Patent number: 9439876Abstract: Methods of inhibiting or preventing the proliferation of a fungus and/or a bacteria in a living organism, in water, in air, and/or on surfaces, include administering or providing a therapeutic amount or an effective amount of an antimicrobial composition including Octodrine.Type: GrantFiled: January 13, 2014Date of Patent: September 13, 2016Assignee: KECK GRADUATE INSTITUTE OF APPLIED LIFE SCIENCESInventors: Mikhail Martchenko, Kevin Jee Kim
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Patent number: 9358540Abstract: An isothermal reaction and analysis system may include a receiver to receive sample holders, a thermal control subsystem to control a temperature of the receiver, an excitation subsystem, a detection subsystem and an analysis subsystem. Excitation sources and/or detectors are positioned to enhance data collection. Sample holders may include filters, selectively blocking and passing wavelengths or bands of electromagnetic radiation.Type: GrantFiled: July 22, 2015Date of Patent: June 7, 2016Assignee: KECK GRADUATE INSTITUTEInventors: Robert W. Doebler, II, Christopher Cooney, Anna Hickerson, James D. Sterling, Ali Nadim
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Publication number: 20150196499Abstract: Methods of inhibiting or preventing the proliferation of a fungus and/or a bacteria in a living organism, in water, in air, and/or on surfaces, include administering or providing a therapeutic amount or an effective amount of an antimicrobial composition including Octodrine.Type: ApplicationFiled: January 13, 2014Publication date: July 16, 2015Applicant: KECK GRADUATE INSTITUTE OF APPLIED LIFE SCIENCESInventors: Mikhail Martchenko, Kevin Jee Kim
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Publication number: 20140206039Abstract: Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.Type: ApplicationFiled: January 28, 2014Publication date: July 24, 2014Applicants: ALDER BIOPHARMACEUTICALS, INC., KECK GRADUATE INSTITUTEInventors: James M. CREGG, John Latham, Mark Litton, Randal Schatzman, Ilya Tolstrorukov
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Patent number: 8784736Abstract: An isothermal reaction and analysis system may include a receiver to receive sample holders, a thermal control subsystem to control a temperature of the receiver, an excitation subsystem, a detection subsystem and an analysis subsystem. Excitation sources and/or detectors are positioned to enhance data collection. Sample holders may include filters, selectively blocking and passing wavelengths or bands of electromagnetic radiation.Type: GrantFiled: March 8, 2012Date of Patent: July 22, 2014Assignee: Keck Graduate InstituteInventors: Robert W. Doebler, II, Christopher Cooney, Anna Hickerson, James D. Sterling, Ali Nadim
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Patent number: 8709756Abstract: Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.Type: GrantFiled: August 30, 2012Date of Patent: April 29, 2014Assignees: Keck Graduate Institute, Alder Biopharmaceuticals, Inc.Inventors: James M. Cregg, John Latham, Mark Litton, Randall Schatzman, Ilya I. Tolstorukov
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Patent number: 8470552Abstract: The present disclosure provides a method for culturing cells in exogenous lactic acid. Certain aspects of the present disclosure include the production of recombinant proteins, such as antibodies and fragments thereof. Certain aspects of the present disclosure also relate to methods of controlling lactic acid production, pH stability and osmolality in cell culture.Type: GrantFiled: October 12, 2010Date of Patent: June 25, 2013Assignee: Keck Graduate InstituteInventors: Matthew S. Croughan, Nathaniel W. Freund
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Patent number: 8268582Abstract: Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.Type: GrantFiled: October 22, 2004Date of Patent: September 18, 2012Assignees: Keck Graduate Institute, Alder Biopharmaceuticals, Inc.Inventors: James M. Cregg, John Latham, Mark Litton, Randall Schatzman′, Ilya I. Tolstorukov