Patents Assigned to Korean Research Institute of Bioscience and Biotechnology
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Patent number: 9914757Abstract: Provided is a methionyl tRNA synthase (MRS) for the biosynthesis of a photomethionine-labeled protein and a method for preparing a photoactivatable protein G variant using same and, more particularly, to an MRS variant in which alanine at the position of 12th is substituted with glycine, leucine at the position of 13th by serine, tyrosine at the position of 260th by phenylalanine, isoleucine at the position of 297th by valine, and histidine at the position of 301st by leucine from the N-terminal of the amino acid sequence of a wild-type Escherichia coli methionyl tRNA synthase. The MRS variant effectively confirms the biosynthesis of a photomethionine (pM)-labeled target protein and thus can be utilized for the biosynthesis of a pM-labeled target protein.Type: GrantFiled: May 29, 2014Date of Patent: March 13, 2018Assignee: Korean Research Institute of Bioscience and BiotechnologyInventors: Myung Kyu Lee, Bong Hyun Chung, Jeong Hee Moon, Ga Bi Lee
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Patent number: 9854804Abstract: Disclosed are a novel strain with antibacterial activity, identified as Streptomyces sp. AN1542 strain (Accession No.: KCTC 12113BP), an antibacterial composition comprising a compound represented by Chemical Formula 1 or 2, or a strain producing the same as an active ingredient, and a method for producing the same. Exhibiting potent antibacterial activity against pathogenic microbes and antibiotic-resistant microbes, particularly, MRSA, QRSA, VRSA and VRE, the compound or the strain of the present invention can be applied to the treatment of infectious diseases caused by superbacteria.Type: GrantFiled: January 4, 2012Date of Patent: January 2, 2018Assignee: KOREAN RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Won Gon Kim, Yun Ju Kwon, Mi Jin Sohn
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Patent number: 9657328Abstract: The present invention relates to a method for modulating thermostability or acid tolerance of a microbe by mutating a nucleic acid molecule encoding a homoserine o-succinyltransferase (MetA) to obtain a nucleic acid molecule encoding a mutant MetA and transforming a microbial cell with the nucleic acid molecule encoding a mutant MetA. The microbe, a bacteria expressing the mutant MetA of the present invention represents a growth rate improved in a vigorous environment such as higher-temperatures and/or lower pH conditions.Type: GrantFiled: September 11, 2009Date of Patent: May 23, 2017Assignee: Korean Research Institute of Bioscience and BiotechnologyInventors: Jae-Gu Pan, Mor Dukhova Elena Anatolievna
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Patent number: 9550997Abstract: The present invention relates techniques for identifying suitable secretion fusion partner (SFP) for hyper-secretory production of recombinant proteins. The SFPs can be obtained from secretome analyzes. Recombinant proteins are produced in a fusion form with a secretion fusion partner (SFP) and can be separated from the SFP by in vitro protease treatment. SFPs of this invention greatly improve the secretion level of target proteins and peptides which are valuable for bio-pharmaceuticals and the bio-industry.Type: GrantFiled: December 4, 2009Date of Patent: January 24, 2017Assignee: Korean Research Institute of Bioscience and BiotechnologyInventors: Jung-Hoon Sohn, Jung-Hoon Bae, Hyun-Jin Kim, Kwang-Mook Lim
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Publication number: 20150148527Abstract: A sugar chain-containing polymer that enables targeting to the lesion area of liver fibrosis and is useful for imaging, diagnosis and therapy of liver fibrosis; and a sugar chain-containing polymer complex comprising the polymer as a carrier for an anionic substance useful fix therapy and the like; are provided. The polymer is a sugar chain-containing polymer which is a cationic polymer comprising an amine, which polymer comprises N-acetylglucosamine bound thereto. The polymer preferably has a disulfide bond. The polymer preferably has a structure in which polyethyleneimine is linked via a disulfide bond.Type: ApplicationFiled: May 27, 2014Publication date: May 28, 2015Applicants: SOMAR Corp., KOREAN RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Bong Hyun CHUNG, Hirohiko ISE, Toshihiro AKAIKE, Sun-Jung KIM
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Patent number: 7816509Abstract: Disclosed are a Pichia pastoris-derived translation elongation factor (TEF) promoter, a recombinant expression vector carrying the promoter and a heterologous protein encoding base sequence operably linked to the promoter, and a host cell transfected or transformed with the recombinant expression vector. Also, a method is provided for producing a heterologous protein. The method comprises culturing the host cell to express the Pichia pastoris-derived TEF promoter and isolating the promoter from the culture. The TEF promoter can be utilized for the mass production of heterologous proteins without the need for inducers.Type: GrantFiled: January 19, 2006Date of Patent: October 19, 2010Assignee: Korean Research Institute of Bioscience & BiotechnologyInventors: Hong Weon Lee, Jung Oh Ahn, Joon Ki Jung, Eui Sung Choi, Chun Suk Kim, Eun Gyo Lee, Ji Yeon Hong, Hyeok Won Lee, Myoung Soo Park
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Publication number: 20100173430Abstract: The present invention relates to an N-terminal cysteine-tagged Streptococcal protein G variant. Since the variant binds in a directional manner to a surface of a biochip or a biosensor, the variant provides a biochip and a biosensor having an improved antibody-immobilizing capability, compared with an untagged protein G variant.Type: ApplicationFiled: June 20, 2007Publication date: July 8, 2010Applicant: KOREAN RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Bong Hyun Chung, Jeong Min Lee, Sun-Ok Jung, Yongwon Jung
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Publication number: 20100151505Abstract: The present invention relates a method for modulating thermostability or acid tolerance of a microbe, comprising the steps of: (a) conferring a substitution mutation to a homoserine o-succinyltransferase (MetA)-encoding nucleotide sequence; and (b) transforming the MetA-encoding nucleotide sequence into a microbial cell. The mutated MetA of the present invention shows stability notably enhanced at high-temperatures and/or under acid conditions or decreased at mild temperature, and the microbe, particular bacteria expressing the mutated MetA of the present invention represents a growth rate improved in a vigorous environment such as higher-temperatures and/or lower acid conditions.Type: ApplicationFiled: September 11, 2009Publication date: June 17, 2010Applicant: Korean Research Institute of Bioscience and BiotechnologyInventors: Jae-Gu Pan, Mor Dukhova Elena Anatolievna
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Patent number: 7563872Abstract: The present invention relates to a protease, and more specifically to a protease derived from Aranicola proteolyticus, a gene coding for said enzyme, a gene expression system for said protease, a process for purifying the protease, and the uses of said protease in industrial applications, such as for example, detergents, cosmetics, leather processing agents, chemicals for laboratory research, solubilizing or softening agents for food, meat modifier, feed or food additives, or oil and fat separating agents, as well as pharmaceutical compositions.Type: GrantFiled: September 12, 2007Date of Patent: July 21, 2009Assignee: Korean Research Institute of Bioscience and BiotechnologyInventors: Ho-Yong Park, Kwang-Hee Son, Doo-Sang Park, Sang-Woon Shin, Hyun-Woo Oh, Mi-Gwang Kim, Dong-Ha Shin
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Publication number: 20080069846Abstract: The present invention relates to a protease, and more specifically to a protease derived from Aranicola proteolyticus, a gene coding for said enzyme, a gene expression system for said protease, a process for purifying the protease, and the uses of said protease in industrial applications, such as for example, detergents, cosmetics, leather processing agents, chemicals for laboratory research, solubilizing or softening agents for food, meat modifier, feed or food additives, or oil and fat separating agents, as well as pharmaceutical compositions.Type: ApplicationFiled: September 12, 2007Publication date: March 20, 2008Applicant: Korean Research Institute of Bioscience and BiotechnologyInventors: Ho-Yong Park, Kwang-Hee Son, Doo-Sang Park, Sang-Woon Shin, Hyun-Woo Oh, Mi-Gwang Kim, Dong-Ha Shin
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Patent number: 7297332Abstract: The present invention relates to a protease, and more specifically to a protease derived from Aranicola proteolyticus, a gene coding for said enzyme, a gene expression system for said protease, a process for purifying the protease, and the uses of said protease in industrial applications, such as for example, detergents, cosmetics, leather processing agents, chemicals for laboratory research, solubilizing or softening agents for food, meat modifier, feed or food additives, or oil and fat separating agents, as well as pharmaceutical compositions.Type: GrantFiled: December 29, 2000Date of Patent: November 20, 2007Assignee: Korean Research Institute of Bioscience and BiotechnologyInventors: Ho-Yong Park, Kwang-Hee Son, Doo-Sang Park, Sang-Woon Shin, Hyun-Woo Oh, Mi-Gwang Kim, Dong-Ha Shin
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Publication number: 20040191861Abstract: The present invention relates to a method of protein synthesis and, more particularly, to a method of effective protein synthesis by regulating the expression of a protein in a host cell, wherein said host cell is transformed with an expression vector comprising a promoter as well as a DNA fragment for a gene that encodes a desired protein, wherein said promoter has an inductive activity for transcription during the resting stage of cell growth and also the induction of said protein expression can be controlled by varying culturing conditions.Type: ApplicationFiled: June 30, 2003Publication date: September 30, 2004Applicant: Korean Research Institute of Bioscience and BiotechnologyInventors: Jae-Gu Pan, Heung-Chae Jung, Sooan Shin