Abstract: A reagent for determination of hydrogen peroxide which comprises (A) a compound represented by the following general formula (I): R1—NH—R2 ??(I) (wherein R1 represents a carbamoyl group or the like, and R2 represents an arylamino group, a heteroarylamino group, or a substituent represented by the following general formula (II): [wherein R3 to R6 represent X—Y—Ra {Ra represents a hydrogen atom, an alkyl group or the like, X represents a single bond, O or the like, and Y represents a single bond, (C?O) or the like}, a cyano group, a halogen atom or the like]>; (B) a compound represented by the general formula (III): [wherein R9 represents a group which can be eliminated by oxidative coupling color-developing reaction with the compound represented by the formula (I), and R7, R8, and R10 to R13 have the same meaning as that of R3], or the like; and (C) a peroxidative substance.

Abstract: The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.

Abstract: The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.

Abstract: The present invention provides a method for the quantitative determination of cholesterol in low density lipoproteins and a reagent kit for use therein. The present invention also provides a method for continuous fractional determination of cholesterol in high density lipoproteins and cholesterol in low density lipoproteins and a reagent kit for use therein, as well as a method for continuous fractional determination of cholesterol in high density lipoproteins and total cholesterol and a reagent kit for use therein.

Abstract: The present invention provides a method and a reagent for the simple and sensitive determination of cholesterol in remnant-like particles in a biological sample without the separation of components of the sample. The method comprises contacting the sample with (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase in the presence of oxygen or an oxidized coenzyme, and measuring the formed hydrogen peroxide or reduced coenzyme. The reagent comprises (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase.

Abstract: The present invention relates to a novel endogenous compound 2-amino-3-[2-(&agr;-mannopyranosyl)indole-3-yl]propionic acid; to a method for testing a biological function by quantitating 2-amino-3-[2-(&agr;-mannopyranosyl)indole-3-yl]propionic acid in a collected biological sample and determining the biological function based on the quantitated values; to an antibody specifically reactive with 2-amino-3-[2-(&agr;-mannopyranosyl)indole-3-yl]propionic acid; to a hybridoma that produces the antibody; to a method for immunologically quantitating 2-amino-3-[2-(&agr;-mannopyranosyl)indole-3-yl]propionic acid in a sample by using the antibody; and to a process for producing a 2-amino-3-[2-(&agr;-mannopyranosyl)indole-3-yl]propionic acid derivative.

Abstract: The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.

Abstract: The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.

Abstract: This invention provides a monoclonal antibody which recognizes an epitope corresponding to amino acids 10 to 220, amino acids 221 to 297, or amino acids 469 to 662, counting from the N-terminus of a human Mx protein MxA, and specifically reacts with the human Mx protein by western blotting, immunoprecipitation or immunocyte staining, and a hybridoma which produces the antibody. The human Mx protein MxA monoclonal antibody of this invention can be used, for example, in the diagnosis of viral infection.

Abstract: A DNA encoding a cell surface polypeptide of Porphyromonas gingivalis, and a recombinant DNA being a DNA having integrated said DNA thereinto. The cell surface polypeptide of the periodontopathic organism useful for prophylaxis and diagnosis of periodontal diseases can be obtained in a large amount by a microorganism containing the recombinant DNA wherein the DNA was integrated.

Abstract: There are provided peptides (each having 5 to 10 amino acid residues) corresponding to fragments derived from the amino acid sequence of the 41 kD sub-unit protein constituting the fimbriae of Porphyromonas gingivalis, or salts of the peptides. Further provided are compositions for diagnosis of periodontal disease containing them, vaccines for prophylaxis of periodontal disease containing part of them, and the oral compositions for prophylaxis or treatment of periodontal disease containing antibodies obtained by immunizing animals with the peptides.Peptides are provided which are lowered in nonspecific reactivity and can be used more safely for the above compositions, compared to the above protein and peptides therefrom having 20 amino acid residues.

Abstract: A reagent, kit and method for the quantative determination of ascorbic acid in a sample uses ascorbate oxidase which catalyzes the reaction of reduced ascorbic acid to oxidized ascorbic acid and hydrogen peroxide. Ascorbic acid in the sample is reacted with oxygen in the presence of the ascorbate oxidase, chromogen and peroxidase. Absorbance is determined and compared with a known calibration curve.

Abstract: Chloride ion is quantitatively determined in a sample by adding to the sample an enzyme having glucokinase activity to thereby eliminate glucose, admixing an .alpha.-amylase which has been deactivated by a chelating agent, and reacting the .alpha.-amylase with maltotetraose, maltohexaose or maltoheptaose.

Abstract: A DNA encoding a cell surface polypeptide of Porphyromonas gingivalis, and a recombinant DNA being a DNA having integrated the encoding DNA thereinto. The cell surface polypeptide of the periodontopathic organism useful for prophylaxis and diagnosis of periodontal diseases can be obtained in a large amount by a microorganism containing the recombinant DNA wherein the DNA was integrated.

Abstract: Provided is a method of determining the amount of cholesterol in high-density lipoprotein (HDL), which comprises reacting an HDL-containing sample with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase in the presence of a reagent for aggregating lipoproteins- except HDL, and determining the amount of hydrogen peroxide or reductive co-enzyme formed therein.

Abstract: Cholesterol in low-density lipoprotein (LDL) or very low-density lipoprotein (VLVL) in a sample is determined in the presence of a sugar compound and/or a protein-solubilizing agent.

Abstract: Disclosed are a method for quantitative determination of bilirubin and a reagent for the method, which are useful in clinical examinations. The method comprises subjecting the bilirubin in a sample to coexist with ascorbate oxidase and a reaction promoter in aqueous medium to thereby oxidize the bilirubin, measuring the change in absorbance of the aqueous medium and comparing the change in absorbance with a calibration curve. The reagent comprises ascorbate oxidase and a reaction promoter.

Abstract: The present invention relates to a method for quantitatively determining a peroxidation-active substance, hydrogen peroxide or a coumarin derivative represented by formula (I) or (II): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6 are the same or different and each represents hydrogen, substituted or unsubstituted lower alkyl, substituted or unsubstituted aralkyl, substituted or unsubstituted aryl or halogen; the method comprising reacting the hydrogen peroxide with the coumarin derivative represented by formula (I) or (II) or its salt in the presence of the peroxidation-active substance, and determining the amount of the light emission or the light intensity from the reaction solution.

Abstract: The present invention relates to a method for quantitatively determining ionized calcium in a sample by using a phospholipase, which comprises carrying out an enzyme reaction with the phospholipase in a buffer comprising a nitrogen heterocycle-binding sulfonic acid having a pK ranging 6.6 to 7.6 or a salt thereof.According to the method of the invention, the amount of ionized calcium in a sample, such as serum, can be accurately determined.

Abstract: The object of the invention is to provide a reagent for quantitatively determining creatine kinase with improved storability in liquid form as well as a method for quantitatively determining creatine kinase with stable measurements. Disclosed are a method for quantitatively determining creatine kinase, which comprises activating creatine kinase in a sample in an aqueous medium in coexistence with a trivalent phosphorus compound and a sulfhydryl-containing compound and then determining creatine kinase activity; a method for stabilizing a sulfhydryl-containing compound, which comprises allowing a trivalent phosphorus compound to coexist with a sulfhydryl-containing compound; and a reagent for quantitatively determining creatine kinase, which comprises a trivalent phosphorus compound, a sulfhydryl-containing compound, and a reaction substrate for creatine kinase.