Patents Assigned to Large Scale Proteomics
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Publication number: 20070059770Abstract: Data acquisition and cataloging are used to classify polypeptides into a reference index or database. The database can be used to identify previously unidentified samples. New polypeptides are characterized and added to the database.Type: ApplicationFiled: November 6, 2006Publication date: March 15, 2007Applicant: LARGE SCALE PROTEOMICS CORPORATION A Division of Large Scale Biology CorporationInventors: Norman Anderson, N. Anderson
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Patent number: 7070739Abstract: A method for separating microorganisms, especially infectious agents, from a mixture by two dimensional centrifugation on the basis of sedimentation rate and isopycnic banding density, for sedimenting such microorganisms through zones of immobilized reagents to which they are resistant, for detecting banded particles by light scatter or fluorescence using nucleic acid specific dyes, and for recovering the banded particles in very small volumes for characterization by mass spectrometry of viral protein subunits and intact viral particles, and by fluorescence flow cytometric determination of both nucleic acid mass and the masses of fragments produced by restriction enzymes. The method is based on the discovery that individual microorganisms, such as bacterial and viral species, are each physically relatively homogeneous, and are distinguishable in their biophysical properties from other biological particles, and from non-biological particles found in nature.Type: GrantFiled: May 16, 2000Date of Patent: July 4, 2006Assignee: Large Scale Proteomics CorporationInventors: Norman G. Anderson, N. Leigh Anderson
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Publication number: 20060032747Abstract: An automated, computer controlled assembly is provided for continuously processing a large number of electrophoresis gels. The assembly includes a loading assembly for loading a gel onto a carrier, a gel staining assembly and a scanning and cutting assembly. The staining assembly and the scanning and cutting assembly each include a robotic arm that is able to capture a gel and transfer the gel to selected work stations and can transfer the gel between the respective robotic arms.Type: ApplicationFiled: July 12, 2005Publication date: February 16, 2006Applicant: LARGE SCALE PROTEOMICS CORPORATION A Division of Large Scale Biology CorporationInventors: N. Anderson, Jack Goodman, L. Wallgren
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Publication number: 20060024234Abstract: Protein markers of toxicity and efficacy for antilipemic drugs are determined. Methods and reagents are disclosed for determining whether a patient receiving an antilipemic drug, especially a statin or HMGCoA reductase inhibiting drug, is experiencing drug efficacy and/or toxicity. Individual susceptibility is also determined prior to treatment. Also, drug discovery of similar acting candidates and their likelihood of being toxic or effective is determined by analysis of all proteins in a sample simultaneously by 2-dimensional gel electrophoresis.Type: ApplicationFiled: September 19, 2005Publication date: February 2, 2006Applicant: LARGE SCALE PROTEOMICS CORPORATION, A Division of Large Scale Biology CorporationInventors: N. Anderson, Sandra Steiner
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Patent number: 6980674Abstract: Data acquisition and cataloging are used to classify polypeptides into a reference index or database. The database can be used to identify previously unidentified samples. New polypeptides are characterized and added to the database.Type: GrantFiled: January 4, 2001Date of Patent: December 27, 2005Assignee: Large Scale Proteomics Corp.Inventors: Norman G. Anderson, N. Leigh Anderson
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Publication number: 20050279632Abstract: Highly hydrophobic compounds and hydrophobic proteins are solubilized in a non-aqueous solvent containing an electrolyte for electrophoretic separation. The non-aqueous solvent is an ionic liquid or a mixture of an organic solvent containing an ionic liquid in an amount to render the solvent electrically conductive and amenable for electrophoretic separation. The hydrophobic proteins are separated by electrophoresis using an electrophoresis gel that is compatible with the organic solvent and ionic liquid.Type: ApplicationFiled: May 23, 2005Publication date: December 22, 2005Applicant: LARGE SCALE PROTEOMICS CORPORATIONInventors: Norman Anderson, James Braatz
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Publication number: 20050239078Abstract: Methods and reagents for simultaneously measuring the concentration of numerous proteins in a sample are described. The technique uses many antibody display phages that contain corresponding specific nucleic acid sequence tags. Binding between various proteins and antibodies is determined by simultaneous detection of the specific sequence tags using a microarray. This method is applicable even when the concentrations of different proteins differ by orders of magnitude as the nucleic acid sequence tags may be amplified.Type: ApplicationFiled: October 20, 2003Publication date: October 27, 2005Applicant: LARGE SCALE PROTEOMICS CORPORATION, a Division of Large Scale Biology CorporationInventor: N. Anderson
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Patent number: 6939453Abstract: Highly hydrophobic compounds and hydrophobic proteins are solubilized in a non-aqueous solvent containing an electrolyte for electrophoretic separation. The non-aqueous solvent is an ionic liquid or a mixture of an organic solvent containing an ionic liquid in an amount to render the solvent electrically conductive and amenable for electrophoretic separation. The hydrophobic proteins are separated by electrophoresis using an electrophoresis gel that is compatible with the organic solvent and ionic liquid.Type: GrantFiled: August 14, 2002Date of Patent: September 6, 2005Assignee: Large Scale Proteomics CorporationInventors: Norman G. Anderson, James A. Braatz
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Patent number: 6933109Abstract: Methods and reagents for rapid purification and/or identification of particles in a liquid sample are described. The technique uses centrifugation to concentrate particles against a slanted surface having an agent specifically binding to the particles. This method is applicable for the rapid identification of viruses and other difficult or impossible to culture microorganisms without replication or amplification of the microorganism.Type: GrantFiled: December 22, 2000Date of Patent: August 23, 2005Assignee: Large Scale Proteomics CorporationInventor: Norman G. Anderson
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Patent number: 6932895Abstract: An automated, computer controlled assembly is provided for continuously processing a large number of electrophoresis gels. The assembly includes a loading assembly for loading a gel onto a carrier, a gel staining assembly and a scanning and cutting assembly. The staining assembly and the scanning and cutting assembly each include a robotic arm that is able to capture a gel and transfer the gel to selected work stations and can transfer the gel between the respective robotic arms.Type: GrantFiled: April 4, 2002Date of Patent: August 23, 2005Assignee: Large Scale Proteomics CorporationInventors: N. Leigh Anderson, Jack Goodman, L. Eric Wallgren
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Publication number: 20050170432Abstract: Data acquisition and cataloging are used to classify polypeptides into a reference index or database. The database can be used to identify previously unidentified samples. New polypeptides are characterized and added to the database.Type: ApplicationFiled: February 9, 2005Publication date: August 4, 2005Applicant: LARGE SCALE PROTEOMICS CORPORATION, A Division of Large Scale Biology CorporationInventors: Norman Anderson, N. Anderson
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Patent number: 6911312Abstract: A method for separating microorganisms, especially infectious agents, from a mixture by two dimensional centrifugation on the basis of sedimentation rate and isopycnic banding density, for sedimenting such microorganisms through zones of immobilized reagents to which they are resistant, for detecting banded particles by light scatter or fluorescence using nucleic acid specific dyes, and for recovering the banded particles in very small volumes for characterization by mass spectrometry of viral protein subunits and intact viral particles, and by fluorescence flow cytometric determination of both nucleic acid mass and the masses of fragments produced by restriction enzymes. The method is based on the discovery that individual microorganisms, such as bacterial and viral species, are each physically relatively homogeneous, and are distinguishable in their biophysical properties from other biological particles, and from non-biological particles found in nature.Type: GrantFiled: January 18, 2002Date of Patent: June 28, 2005Assignee: Large Scale Proteomics CorporationInventors: Norman G. Anderson, N. Leigh Anderson
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Patent number: 6887701Abstract: The microarrays of the present invention are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that is glued to a backing.Type: GrantFiled: June 14, 2001Date of Patent: May 3, 2005Assignee: Large Scale Proteomics CorporationInventors: Norman G. Anderson, N. Leigh Anderson
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Patent number: 6846635Abstract: Microarrays are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that is glued to a backing.Type: GrantFiled: July 28, 2000Date of Patent: January 25, 2005Assignee: Large Scale Proteomics Corp.Inventors: Norman G. Anderson, N. Leigh Anderson, James A. Braatz
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Publication number: 20040262514Abstract: A mass spectrometry apparatus uses image processing of output signals of a camera in a mass spectrometer to provide feedback for directing the laser. The present invention provides for the determination of where samples have actually been deposited on a plate, and for the selection of different points for each sample, based on its structure, at which to aim a laser, during the cycle period of the mass spectrometer. Such feedback information increases the likelihood that the laser impinges samples and provides useful data.Type: ApplicationFiled: July 27, 2004Publication date: December 30, 2004Applicant: LARGE SCALE PROTEOMICS CORPORATION, A Division of Large Scale Biology CorporationInventors: John J. Lennon, Anthony James Makusky, Samuel G. Michael
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Patent number: 6804410Abstract: A mass spectrometry apparatus uses image processing of output signals of a camera in a mass spectrometer to provide feedback for directing the laser. The present invention provides for the determination of where samples have actually been deposited on a plate, and for the selection of different points for each sample, based on its structure, at which to aim a laser, during the cycle period of the mass spectrometer. Such feedback information increases the likelihood that the laser impinges samples and provides useful data.Type: GrantFiled: April 17, 2001Date of Patent: October 12, 2004Assignee: Large Scale Proteomics CorporationInventors: John J. Lennon, Anthony James Makusky, Samuel G. Michael
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Patent number: 6783648Abstract: An apparatus for expressing and unloading an isoelectric focusing gel from an electrophoresis gel tube includes a first support for supporting the gel tube, a plunger rod and a second support for supporting the plunger rod. The first support is mounted on a movable carriage and is moved toward the second support so that the gel tube slides onto the plunger rod to unload the gel from the gel tube. A plurality of gel tubes can be mounted in a rack and the rack coupled to the first support. The first support preferably includes a plurality of openings oriented with the gel tubes for guiding a respective plunger rod through the axial passage of the gel tubes. In preferred embodiments, the second support supporting the plunger rods is substantially stationary while the first support moves toward the second support so that the gel tubes slide onto the plunger rods.Type: GrantFiled: September 1, 2000Date of Patent: August 31, 2004Assignee: Large Scale Proteomics CorporationInventors: N. Leigh Anderson, Jack Goodman
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Patent number: 6761810Abstract: An automated assembly for performing first dimension electrophoresis is described herein that includes a supply magazine, an electrophoresis tank and an automated transferring device that robotically transfers biological samples from sample vials retained in the supply magazine, and delivers the biological samples one by one to tube gels supported in a rack within the electrophoresis tank. The transferring device is configured to move in three dimensions with respect to the supply magazine and the rack for flexible sample delivery.Type: GrantFiled: March 9, 2001Date of Patent: July 13, 2004Assignee: Large Scale Proteomics Corp.Inventors: Andrew McGrath, N. Leigh Anderson, Jack Goodman
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Patent number: 6758804Abstract: Devices and methods for removing portions of gradients relate to a float with an upper concave surface for collecting the gradient portion.Type: GrantFiled: July 27, 2001Date of Patent: July 6, 2004Assignee: Large Scale ProteomicsInventor: Norman G. Anderson
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Patent number: 6734424Abstract: An automated pipetting apparatus and method for forming sample spots on a support include a pipette. A robotic assembly moves a sample container, such as a multiwell microtiter plate, and the support to the pipette for receiving and dispensing liquid samples. The pipette draws a predetermined volume of the liquid sample from the sample container into the axial passage of the pipette and forms a pocket of a gaseous material above and forms a barrier material below the volume of the liquid sample. The liquid sample is drawn into the pipette a distance sufficient to form a space between the liquid sample and the outlet of the pipette and to contain the liquid sample completely within the pipette.Type: GrantFiled: May 16, 2002Date of Patent: May 11, 2004Assignee: Large Scale Proteomics CorporationInventors: John Lennon, Taraneh Norouzi