Abstract: A method for one-pot one-step assembly of two or more DNA molecules to form at least one recombinant DNA molecule, and a substrate and a kit for this purpose. A simple and cost-effective assembly method for DNA molecules. A method for one-pot one-step assembly of two or more DNA molecules to form at least one recombinant DNA molecule is provided, wherein the two or more DNA molecules to be assembled are brought together in dry form with a suitable reaction medium on at least one substrate present in a reaction vessel.
Abstract: The present invention relates to genetically modified enzymes obtained by rational design of the active site binding pocket of the prototypic enzyme 4-hydroxyphenylacetate 3-hydroxylase (4HPA3H) for hydroxylating a 4-hydroxyphenyl compound to yield a 3,4-dihydroxyphenyl compound and to biotechnological methods including in vivo and in vitro methods using said enzymes or catalytically active fragments thereof. Further provided is a method either using a suitable oxidase or hydroxylase further enabling the subsequent site specific methylation of the 3,4-dihydroxyphenyl compound in a coupled enzymatic reaction by providing a suitable O-methyltransferase. Finally, compositions obtainable by the aforementioned methods are disclosed.
Type:
Grant
Filed:
September 30, 2016
Date of Patent:
April 27, 2021
Assignees:
SYMRISE AG, LEIBNIZ-INSTITUT FÜR PFLANZENBIOCHEMIE
Inventors:
Ludger A. Wessjohann, Susann Herrmann, Martin Dippe, Torsten Geissler, Katrin Geissler, Jakob Peter Ley
Abstract: The invention relates to the use of a compound of formula (I), or a composition containing the compound, as glutaminyl cyclase (QC) inhibitor and to methods of preparation.
Type:
Application
Filed:
September 15, 2016
Publication date:
August 16, 2018
Applicants:
Hochschule Anhalt, Leibniz-Institut Für Pflanzenbiochemie (IPB)
Inventors:
Stephanie Hielscher-Michael, Carola Griehl, Hans-Ulrich Demuth, Stephan Schilling, Ludger Wessjohann, Norbert Arnold
Abstract: The present invention relates to isolated polypeptides that are derived from wildtype Bacillus subtilis S-Adenosylmethionine (SAM) synthase or from a biologically active fragment thereof, wherein said isolated polypeptides comprise an amino acid sequence that, in relation to the amino acid sequence of said wildtype Bacillus subtilis SAM synthase or of the biologically active fragment thereof, comprises at least one amino acid substitution, selected from the group consisting of amino acid substitutions at positions I317 and I105. The present invention further relates to respective isolated nucleic acids, vectors, host cells, uses and methods for the production of SAM derivatives.
Type:
Grant
Filed:
September 4, 2014
Date of Patent:
October 31, 2017
Assignee:
LEIBNIZ-INSTITUT FÜR PFLANZENBIOCHEMIE STIFTUNG DES ÖFFENTLICHEN RECHTS
Inventors:
Martin Dippe, Wolfgang Brandt, Ludger A. Wessjohann, Hannes Rost, Andrea Porzel