Patents Assigned to Life Technologies AS
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Patent number: 11913066Abstract: Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.Type: GrantFiled: January 26, 2022Date of Patent: February 27, 2024Assignee: Life Technologies CorporationInventors: Robert Green, Dennis Wang, Julio Mulero, Robert Lagace
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Publication number: 20240058714Abstract: The present invention discloses a multi-functional toy comprising: a flexible shell comprising a receiving cavity, an opening communicating with the receiving cavity, and a locking portion provided around the opening; a seat is mounted on the opening and protrudes into the receiving cavity; the seat comprises a clamping groove formed by being recessed and an upper clamping wall and a lower clamping wall forming the clamping groove; the locking portion protrudes into the clamping groove; the upper clamping wall protrudes into the receiving cavity; the lower clamping wall is exposed to the flexible shell; and the lamp holder further comprises a plurality of fasteners penetrating upwards successively through the lower clamping wall and the locking portion.Type: ApplicationFiled: May 10, 2023Publication date: February 22, 2024Applicant: Guangdong Time Life Technology Co., Ltd.Inventor: Longcai XU
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Patent number: 11904321Abstract: A container includes a lower portion and a lid to connect with the lower portion. The lid includes a fluidic interface, a swing bar, and a counter structure disposed on an opposite side of the fluidic interface relative to the swing bar. The fluidic interface includes a first liquid port, a second liquid port, a gas port, and a seal ring disposed around the first liquid port, the second liquid port, and the gas port, the first liquid port disposed along a center axis of the container.Type: GrantFiled: May 28, 2020Date of Patent: February 20, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Mark Reed, Rui Zheng
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Patent number: 11905558Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.Type: GrantFiled: August 14, 2020Date of Patent: February 20, 2024Assignee: Life Technologies CorporationInventors: Mark Reed, Chiu Tai Andrew Wong, David Marran
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Patent number: 11905557Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.Type: GrantFiled: March 5, 2021Date of Patent: February 20, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Swati Goyal, Achim Karger, Peter Ma, S. Jeffrey Rosner, Ian Walton, Jonathan Wang, Michael Wenz
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Patent number: 11906724Abstract: Apparatuses, systems and methods for generating color video with a monochrome sensor include the acts of (i) selectively energizing each of a plurality of light sources in a sequence, (ii) capturing a monochrome image of the illuminated sample at a monochrome sensor at each stage of the sequence, and (iii) generating a color video from the monochrome images. The sequence can have a series of stages with each stage of the sequence corresponding to activation of a different wavelength of light from the light sources to illuminate a sample. Generating the monochrome video can include the acts of compiling a plurality of monochrome images captured at the monochrome sensor with a single light source into a series of monochrome video frames comprising the monochrome video.Type: GrantFiled: May 6, 2022Date of Patent: February 20, 2024Assignee: Life Technologies CorporationInventors: Marc Berretta, Jason Mecham, Frank Metting, Jonathan Paullin, Paul Boeschoten, Erik Persmark, Eric Altendorf
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Patent number: 11899029Abstract: The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. In a preferred embodiment, the present teachings provide apparatuses and methods for automated enrichment of templated beads produced by PCR.Type: GrantFiled: November 17, 2022Date of Patent: February 13, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Kristopher Barbee, Maximilian Carpino, Ray Wheeling, Nicholas Peter Bajka
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Publication number: 20240042062Abstract: This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.Type: ApplicationFiled: October 16, 2023Publication date: February 8, 2024Applicants: Life Technologies Corporation, Pierce Biotechnology, Inc.Inventors: Surbhi Desai, Marie Christine Nlend, Kyle Gee, Matthew Baker, Robert Aggeler, Scott Sweeney, Aleksey Rukavishnikov, Shih-Jung Huang
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Patent number: 11894105Abstract: A method for compressing nucleic acid sequence data wherein each sequence read is associated with a molecular tag sequence, wherein a portion of the sequence reads alignments correspond to sequence reads mapped to a targeted fusion reference sequence includes determining a consensus sequence read for each family of sequence reads based on flow space signal measurements corresponding to the family of sequence reads, determining a consensus sequence alignment for each family of sequence reads, wherein a portion of the consensus sequence alignments correspond to the consensus sequence reads aligned with the targeted fusion reference sequence, generating a compressed data structure comprising consensus compressed data, the consensus compressed data including the consensus sequence read and the consensus sequence alignment for each family, and detecting a fusion using the consensus sequence reads and the consensus sequence alignments from the compressed data structure.Type: GrantFiled: September 20, 2018Date of Patent: February 6, 2024Assignee: Life Technologies CorporationInventors: Rajesh Gottimukkala, Cheng-Zong Bai, Dumitru Brinza, Jeoffrey Schageman, Varun Bagai
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Patent number: 11890557Abstract: A filter bag assembly includes: a flexible first sheet; a flexible second sheet overlying and secured to the first sheet so that a compartment is formed therebetween; a porous filter sheet disposed between the first sheet and the second sheet so as to divide the compartment into a first compartment and a second compartment; a first port secured to the second sheet so as to communicate directly with the first compartment; and a second port secured to the second sheet so as to communicate directly with the second compartment, the second port being spaced apart from the first port. The porous filter sheet is disposed so that fluid entering the first compartment through the first port must pass through the filter sheet before entering the second compartment or exiting through the second port.Type: GrantFiled: May 10, 2022Date of Patent: February 6, 2024Assignee: Life Technologies CorporationInventor: Charles R. Rhodes
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Patent number: 11887699Abstract: A method for compressing molecular tagged sequence data includes: grouping sequence reads associated with a molecular tag sequence to form a family of sequence reads, corresponding vectors of flow space signal measurements and corresponding sequence alignments, calculating an arithmetic mean of the corresponding vectors of flow space signal measurements to form a vector of consensus flow space signal measurements, calculating a standard deviation of the corresponding vectors of flow space signal measurements to form a vector of standard deviations, determining a consensus base sequence based on the vector of consensus flow space signal measurements, determining a consensus sequence alignment and generating a compressed data structure comprising consensus compressed data, the consensus compressed data including for each family, the consensus base sequence, the consensus sequence alignment, the vector of consensus flow space signal measurements, the vector of standard deviations and the number of members.Type: GrantFiled: September 16, 2022Date of Patent: January 30, 2024Assignee: Life Technologies CorporationInventor: Cheng-Zong Bai
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Publication number: 20240029899Abstract: Methods for forecasting case counts for a future date in one or more geographic areas of persons infected by a disease is disclosed. The presence of the disease in a biological sample is testable by a polymerase chain reaction (PCR) test. A load of one or more pathogens associated with the disease correlates with a PCR cycle which indicates presence of the one or more pathogens, and is referred to as a threshold cycle (Ct). Data relevant to forecasting the case counts including Ct data and other data is received. The Ct data comprises Ct values from PCR tests of biological samples from persons within the one or more geographic areas. Arrays of feature data for processing by a trained machine learning model are generated, comprising Ct features and other features obtained from the data. A forecasted number of infected persons are generated by processing the arrays using machine learning.Type: ApplicationFiled: July 21, 2023Publication date: January 25, 2024Applicant: Life Technologies CorporationInventors: Mahfuza Sharmin, Manimozhi Manivannan, David Woo, Imran Mujawar, Manoj Gandhi
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Patent number: 11879156Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.Type: GrantFiled: August 10, 2022Date of Patent: January 23, 2024Assignee: Life Technologies CorporationInventors: Phil Waggoner, James A. Ball, Wolfgang Hinz, Michael L. Minto, Scott Parker, David M. Cox, Alexander Mastroianni, Jeremy Gray, Marc Glazer, Kimberly Gorrell
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Patent number: 11874209Abstract: An isolation device for isolation of target particles from a plurality of liquid samples includes a plurality of isolation chips and a vacuum system. Each of the plurality of isolation chips includes a sample reservoir, and a first outlet and a second outlet disposed at opposite sides of the sample reservoir. The vacuum system includes a first vacuum pump connected to the first outlet of each of the plurality of isolation chips and a second vacuum pump connected to the second outlet of each of the plurality of isolation chips. The first vacuum pump generates a negative pressure in each of the plurality of isolation chips through a corresponding first outlet. The second vacuum pump generates a negative pressure in each of the plurality of isolation chips through a corresponding second outlet. The target particles are isolated from each of the plurality of liquid samples in a corresponding sample reservoir.Type: GrantFiled: January 14, 2022Date of Patent: January 16, 2024Assignee: Shenzhen Huixin Life Technologies Co., LtdInventor: Yuchao Chen
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Patent number: 11874250Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.Type: GrantFiled: August 31, 2022Date of Patent: January 16, 2024Assignee: Life Technologies CorporationInventors: Jonathan M. Rothberg, Wolfgang Hinz, John F. Davidson, Antoine M. van Oijen, John Leamon, Martin Huber, Mark James Milgrew, James Bustillo
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Patent number: 11873526Abstract: A reagent solution includes water, a nucleotide, and tris(2-carboxyethyl)phosphine in a range of 0.5 ?M to 1000 ?M. The reagent solution can further include a non-ionic surfactant in an amount of 0.001% to 1% or a biocidal agent in an amount of 0.001% to 1%. The reagent solution can include salts, such as sodium chloride or magnesium sulfate.Type: GrantFiled: January 7, 2022Date of Patent: January 16, 2024Assignee: Life Technologies CorporationInventors: Jamsheed Ghadiri, Karta Atehortua-Khalsa
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Patent number: 11872285Abstract: The present invention provides lipid aggregate compositions having at least two cationic lipids in combination with one or optionally more than one non-cationic lipids for the in vitro and/or in vivo delivery of biologically active molecules including nucleic acids, specifically DNA and RNA, and proteins into cells and tissues. Methods are also provided that use the compounds of the present invention to deliver biologically active molecules, into cells and tissues to facilitate the expression of target proteins therein. In some non-limiting embodiments, the subject lipid aggregate compositions can be used to deliver nucleic acid molecules to facilitate the expression of proteins involved in cellular reprogramming and genome editing applications.Type: GrantFiled: October 5, 2020Date of Patent: January 16, 2024Assignee: Life Technologies CorporationInventors: Xavier de Mollerat du Jeu, Nektaria Andronikou
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Patent number: D1011414Type: GrantFiled: August 16, 2021Date of Patent: January 16, 2024Assignee: ELIA Life Technology PBCInventors: Benjamin Reed DeWinter, Adam Linn, Byron Johnson, Andrew J. Chepaitis
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Patent number: D1012960Type: GrantFiled: July 23, 2021Date of Patent: January 30, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Justin Hicks, Matthew Shallice, Laurel Stone, Charysse Archer
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Patent number: D1016098Type: GrantFiled: March 12, 2021Date of Patent: February 27, 2024Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Nephi D. Jones, Jason D. Brown