Patents Assigned to Life Technologies Corporation
  • Methods for manipulating biomolecules
    Patent number: 11542535
    Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.
    Type: Grant
    Filed: June 25, 2014
    Date of Patent: January 3, 2023
    Assignee: Life Technologies Corporation
    Inventors: Zhoutao Chen, Xiaoping Duan, Kyusung Park
  • Labeled enzyme compositions, methods and systems
    Patent number: 11542549
    Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are labeled polymerase conjugates including a polymerase linked to one or more labels, wherein the conjugate has polymerase activity. Such conjugates can exhibit enhanced biological activity and/or superior detectability as compared to conventional labeled polymerases. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.
    Type: Grant
    Filed: February 28, 2018
    Date of Patent: January 3, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Theo Nikiforov
  • Control nucleic acids, and compositions, kits, and uses thereof
    Patent number: 11542540
    Abstract: Provided are methods, as well as compositions, kits, and systems for preparing optimized control nucleic acids (polynucleotides) having reduce nucleic acid damage. Provided nucleic acid compositions provide reduced artifacts as compared to nucleic acid compositions prepared by conventional methods. Provided compositions are useful control in a variety of applications, including, but not limited to sequencing workflows to effectively monitor sensitivity, accuracy and/or precision of data.
    Type: Grant
    Filed: June 12, 2018
    Date of Patent: January 3, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Dalia Dhingra, Richard Chien
  • OPTICAL SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS
    Publication number: 20220412890
    Abstract: An instrument for processing and/or measuring a biological process comprises a plurality of filter assemblies configured to be interchangeably located along at least one of the optical paths. The plurality of filter components includes a first filter assembly characterized by a first optical power and a first filter having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly is characterized by a second optical power and a second filter having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength.
    Type: Application
    Filed: May 26, 2022
    Publication date: December 29, 2022
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kevin MAHER, Mingsong CHEN, Jacob K. FREUDENTHAL, Kuan Moon BOO, Soo Yong LAU, David FORTESCUE, Ming SHEN, Woon Liang Terence SOH
  • High data rate integrated circuit with transmitter configuration
    Patent number: 11536688
    Abstract: A high data rate integrated circuit, such as an integrated circuit including a large sensor array, may be implemented using clock multipliers in individual power domains, coupled to sets of transmitters, including a transmitter pair configuration. Reference clock distribution circuitry on the integrated circuit distributes a relatively low speed reference clock. In a transmitter pair configuration, each pair comprises a first transmitter and a second transmitter in a transmitter power domain. Also, each pair of transmitters includes a clock multiplier connected to the reference clock distribution circuitry, and disposed between the first and second transmitters, which produces a local transmit clock.
    Type: Grant
    Filed: March 3, 2020
    Date of Patent: December 27, 2022
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Keith G. Fife, Jungwook Yang
  • COMPOSITIONS AND METHODS FOR MULTIPLEX RT-PCR AND GENETIC ANALYSIS
    Publication number: 20220403446
    Abstract: The present invention is directed to compositions, methods and kits useful for the generation of nucleic acids from RNA templated and further nucleic acid amplification and detection. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase polymerase chain reaction (RT-PCR). Provided are compositions and methods for improved amplification of nucleic acid molecules in a two-step, addition only RT-PCR procedure. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules, and is useful for a variety of research, industrial, medical and forensic purposes.
    Type: Application
    Filed: August 28, 2020
    Publication date: December 22, 2022
    Applicant: Life Technologies Corporation
    Inventor: Sihong Chen
  • Control system and method for a fluid mixing apparatus
    Patent number: 11529598
    Abstract: The present set of embodiments relate to a system, method, and apparatus controlling a cell culture media mixing system. The control system includes an integrated control unit capable of controlling a broad ranges of peripheral devices commonly used in bioproduction through a graphical user interface displayed on a touch sensitive screen. The bioproduction system is designed to be highly customizable through process modification and recipe creation by user with little or no knowledge of programming and is capable of controlling a wide variety of devices using a single unit. The bioproduction system allows for auto-detection, auto-calibration, and automatic of device related processes into a bioproduction workflow.
    Type: Grant
    Filed: May 29, 2019
    Date of Patent: December 20, 2022
    Assignees: Life Technologies Corporation, Life Technologies Holdings PTE Limited
    Inventors: Joshua Mead, Scott Rickes, Sean Zimmerman, Alessandra Barbosa, Mio Ling, Woon Liang Soh, Kevin Mullen, Christopher Brau, Jordan Cobia, Harmon Jr Cosme Sicat, Kok Shyong Chong, Han Wei, Wei Fuh Teo, Syed Muhammad Baber Ali
  • Methods and apparatus for measuring analytes using large scale FET arrays
    Patent number: 11530444
    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.
    Type: Grant
    Filed: May 22, 2020
    Date of Patent: December 20, 2022
    Assignee: Life Technologies Corporation
    Inventors: Jonathan M. Rothberg, Wolfgang Hinz, Kim L. Johnson, James Bustillo, John Leamon, Jonathan Schultz
  • SYSTEMS AND METHODS FOR IMAGING AND ABLATING A SAMPLE
    Publication number: 20220399197
    Abstract: Disclosed herein are systems for imaging and ablating a sample. An imaging/ablating device (110) includes an optical assembly (112), a sample stage (114), and a receiver (116). The optical assembly (112) is disposed in an inverted position below the sample stage (114) and the receiver (116) is positioned above the sample stage (112). The optical assembly enables imaging of a sample disposed on the sample stage (114). The optical assembly (112) also enables ablation of a region of interest within the sample. The laser light propagated from the optical assembly during ablation propagates substantially in the same direction as the direction of travel of the ablation plume (20) toward the receiver (116).
    Type: Application
    Filed: November 10, 2020
    Publication date: December 15, 2022
    Applicants: Thermo Fisher Scientific (Bremen) GmbH, Life Technologies Corporation, FEI Deutschland GmbH
    Inventors: Alexander Makarov, Michael WARD, Rainer DAUM
  • MODELS FOR ANALYZING DATA FROM SEQUENCING-BY-SYNTHESIS OPERATIONS
    Publication number: 20220383983
    Abstract: Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled.
    Type: Application
    Filed: June 3, 2022
    Publication date: December 1, 2022
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Todd REARICK, Jonathan SCHULTZ
  • Method and apparatus for automated sample preparation
    Patent number: 11513133
    Abstract: The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. In a preferred embodiment, the present teachings provide apparatuses and methods for automated enrichment of templated beads produced by PCR.
    Type: Grant
    Filed: November 16, 2018
    Date of Patent: November 29, 2022
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kristopher Barbee, Maximilian Carpino, Ray Wheeling, Nicholas Bajka
  • System and method for high-yield transient expression in mammalian cells
    Patent number: 11498949
    Abstract: High-yield mammalian transient expression systems can include a cell culture media (particularly serum free, non-animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.
    Type: Grant
    Filed: July 26, 2018
    Date of Patent: November 15, 2022
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Sanjay Vasu, Chao Yan Liu, Jeffrey Rogers, Maria Cisneros, Jingqiu Li, Henry Chiou, Meredith Jones
  • Chemical sensor array having multiple sensors per well
    Patent number: 11499938
    Abstract: In one embodiment, a device is described. The device includes a material defining a reaction region. The device also includes a plurality of chemically-sensitive field effect transistors have a common floating gate in communication with the reaction region. The device also includes a circuit to obtain respective output signals from the chemically-sensitive field effect transistors indicating an analyte within the reaction region.
    Type: Grant
    Filed: October 14, 2020
    Date of Patent: November 15, 2022
    Assignee: Life Technologies Corporation
    Inventors: Jonathan M. Rothberg, Keith G Fife, Jordan Owens, James Bustillo
  • Coated substrate for biological reaction systems
    Patent number: 11493526
    Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.
    Type: Grant
    Filed: April 20, 2020
    Date of Patent: November 8, 2022
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Eliodor Ghenciu, Theodore E. Straub, Evan W. Foster, Michael C. Pallas
  • Heat exchanger system with flexible bag
    Patent number: 11492582
    Abstract: A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. A second inlet port, a second outlet port, and a second drain port are coupled with the flexible bag so as to communicate with the second compartment.
    Type: Grant
    Filed: June 10, 2020
    Date of Patent: November 8, 2022
    Assignee: Life Technologies Corporation
    Inventors: Clinton C. Staheli, Nephi D. Jones, Michael E. Goodwin
  • Reagent mixer and fluid control devices
    Patent number: 11478786
    Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
    Type: Grant
    Filed: August 27, 2020
    Date of Patent: October 25, 2022
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jonathan Schultz, Todd Roswech
  • Biological analysis system and methods
    Patent number: 11480584
    Abstract: A system for biological analysis includes a housing, a block assembly within the housing having a sample block and a baseplate, a heated cover and a cover carrier. The sample block receives a sample holder comprising an RFID tag. A first drive mechanism generates relative movement between the sample block and the baseplate along a first axis. A second drive mechanism generates relative movement between the heated cover and the cover carrier along a second axis that is different from the first axis. Based on a first command the first drive mechanism releasably engages the sample block and operates the second drive mechanism to releasably engage the heated cover with the cover carrier. The system also includes first and second RFID antennas that receive RFID data from the sample holder RFID tag that is read by at least one RFID reader.
    Type: Grant
    Filed: January 30, 2020
    Date of Patent: October 25, 2022
    Assignees: LIFE TECHNOLOGIES HOLDINGS PTE LIMITED, LIFE TECHNOLOGIES CORPORATION
    Inventors: Kuan Moon Boo, Wei Fuh Teo, Zeqi Tan, Soo Yong Lau, Ming Tiong Sia, Woon Liang Soh, Ching Yee Lam, Shan Hua Dong, Marc Haberstroh, Michael C. Pallas, Hardeep Sangha
  • Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
    Patent number: 11474070
    Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: October 18, 2022
    Assignee: Life Technologies Corporation
    Inventors: Marcin Sikora, Melville Davey, Christian Koller, Simon Cawley, Alan Williams, David Kulp
  • Amine-containing transfection reagents and methods for making and using same
    Patent number: 11464863
    Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.
    Type: Grant
    Filed: August 30, 2019
    Date of Patent: October 11, 2022
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Zhiwei Yang, Parul Angrish, Xavier de Mollerat du Jeu, Kristin Wiederholt
  • Aerosol sampling system cartridge
    Patent number: D974524
    Type: Grant
    Filed: January 21, 2021
    Date of Patent: January 3, 2023
    Assignees: THERMO ENVIRONMENTAL INSTRUMENTS LLC, LIFE TECHNOLOGIES CORPORATION
    Inventors: Jeffrey Ambs, Robert Bailey, Emily Pearce