Abstract: A method of preparing adipose tissue for transplantation, from lobular fat extracted, for instance, by liposuction, the fat including a fluid component that also includes an oily component, a blood component and/or sterile solutions and of a solid component that includes cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, characterized in that it includes at least one step of forming an emulsion of the fluid component in the sterile washing solution by active and/or passive stirring and discharging the emulsion from the outlet through a density gradient.

Abstract: The invention relates to a process for preparing a non-expanded tissue derivative, that is not subjected to cell proliferation in vitro, comprising a vascular-stromal fraction enriched in stem and multipotent elements, such as pericytes and/or mesenchymal stem cells, or for preparing non-embryonic stem cells obtained from a tissue sample or from said derivative, wherein said tissue derivatives or said cells are subjected to vibrations derived from a heart sound such to control the degree of differentiation or possible differentiation of said stem and multipotent elements into several other types of cells that is to optimize their potency. The invention relates also to a device for carrying out said process, to stem cells obtainable by the process as well as a drug for the regeneration of an animal tissue.

Abstract: A device and a method for preparing adipose tissue for transplantation from lobular fat extracted, for instance by liposuction, the fat including a fluid component that have an oily component, a blood component and/or sterile solutions, and a solid component including cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, wherein the device includes at least one washing and separating container having a washing chamber, the container has an inlet and an outlet for the liposuctioned material to enter the washing chamber through the inlet and for at least part of the material to exit the washing chamber through the outlet, the washing chamber including stirring means for forming an emulsion of fluid components.

Abstract: A device for preparing adipose tissue for transplantation from lobular fat extracted, for instance by liposuction, said fat consisting of a fluid component comprising an oily component, a blood component and/or sterile solutions and a solid component comprising cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, comprising at least one washing and separating container (1) having a washing chamber (101) for washing the liposuctioned material, which container (1) has an inlet (102) and an outlet (103) for the liposuctioned material to enter the washing chamber (101) through the inlet (102) and for at least part of said material, particularly the fluid component, to exit said chamber (101) through the outlet (103), said washing chamber (101) including means for forming an emulsion of fluid components, by mechanical stirring.

Abstract: A method of preparing adipose tissue for transplantation, from lobular fat extracted, for instance, by liposuction, said fat consisting of a fluid component comprising an oily component, a blood component and/or sterile solutions and of a solid component comprising cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, characterized in that it includes at least one step of washing the cell aggregates carried out at the same time as a step of separating the fluid component, in emulsion form, from the solid component.

Abstract: A device for preparing adipose tissue for transplantation, from lobular fat extracted, for instance, by liposuction. The device comprises at least one washing and separating container (1) having a washing chamber (11) for washing the liposuctioned material, which washing and separating container (1) has at least one inlet (12) and at least one outlet (13) for the liposuctioned material to enter the washing chamber (11) through the inlet (12) and for at least part of said material, the fluid component, to exit said chamber 11 through the outlet 13. The washing and separating container (1) is coupled to stirring means via coupling means (2) for releasably coupling said washing and separating container (1) to said stirring means, drive means being provided for driving said stirring means.

Abstract: A device and a method for preparing adipose tissue for transplantation from lobular fat material extracted by liposuction, the fat material consisting of a mixture of fluid materials and cell fragments, cells and one or more cell magroagglomerates of heterogeneous sizes, include at least one sterile container having a size reducing device for reducing the fat material into cell agglomerates of smaller and identical or similar sizes, the device dividing the inner chamber of the container into at least two portions, particularly a first upper portion and a second lower portion.

Abstract: Chemical preconditioning process for cell material to obtain chemical epigenetic reprogramming and pluripotency expression of stem and multipotent elements of non-embryonic origin, such as pericytes and/or mesenchymal stem cells, and/or adult somatic cells. The process includes the step of subjecting a non-expanded tissue derivative having the stem and multipotent elements, and/or of subjecting non-embryonic stem cells and/or non-embryonic somatic cells, obtained from a tissue sample or from the derivative, to a mixing with hyaluronic acid esterified with butyric and retinoic acids (HBR).

Abstract: A device and a method for preparing adipose tissue for transplantation from lobular fat extracted, for instance by liposuction, the fat including a fluid component that have an oily component, a blood component and/or sterile solutions, and a solid component including cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, wherein the device includes at least one washing and separating container having a washing chamber, the container has an inlet and an outlet for the liposuctioned material to enter the washing chamber through the inlet and for at least part of the material to exit the washing chamber through the outlet, the washing chamber including stirring means for forming an emulsion of fluid components.

Abstract: A device and a method for preparing adipose tissue for transplantation from lobular fat extracted, for instance by liposuction, the fat including a fluid component that have an oily component, a blood component and/or sterile solutions, and a solid component including cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, wherein the device includes at least one washing and separating container having a washing chamber, the container has an inlet and an outlet for the liposuctioned material to enter the washing chamber through the inlet and for at least part of the material to exit the washing chamber through the outlet, the washing chamber including stirring means for forming an emulsion of fluid components.

Abstract: Preparation and method for producing a preparation or a tissue derivative that include mesenchymal stem cells, to be used in cellular therapy, for cosmetic treatments, for replacing a tissue or an organ, or inducing or accelerating tissue repair or regeneration. The method includes the steps of extracting tissue containing mesenchymal stem cells, such as adipose tissue, from a cadaveric donor by liposuction or by surgical removal of parts of adipose tissue, and mechanically treating tissue to provide a fluid component, having an oily component, a blood component and/or a sterile solution, and a solid component having cell fragments, cells and one or more cell macro-agglomerates of heterogeneous sizes, thereby separating and removing the fluid component from the solid component, which generates an emulsion of fluid components.