Abstract: N-(3-(3-.sup.125 iodo, 4-hydroxybenzyl)propionyl)phosphatidylethanolamine[.sup.125 I-phenylpropionyl-PE], a nonexchangeable iodinated lipid of high specific radioactivity is disclosed, with a method for its synthesis as well as the synthesis of other iodinated lipids using a technique involving acylation of the amine nitrogen of a phospholipid, such as phosphatidylethanolamine, with an [.sup.125 I] or [.sup.131 I] substituted cyclic organic acid, for example, monoiodinated Bolton-Hunter reagent and purification by thin layer chromatography. The iodinated lipid may be quantitatively incorporated into vesicles for use as an accurate liposome tracer in in vitro and in vivo assays that require high levels of sensitivity unobtainable with most other lipid-labeling techniques.

Abstract: An open reading frame DNA vector is presented having a promoter-translation start region, and adjacent thereto and downstream therefrom, a first coding segment having a start codon, a second coding segment coding for detectable protein, and an insertion region between said first and second coding regions having an insertion site and being of such length that the first and second coding segments are not in reading frame phase with each other. A method for the cloning and positive selection of a DNA segment having an open reading frame is also presented, as well as a tribrid protein wherein each terminal region and the mid-section is derived from a different gene, and a method of identifying and quantifying a protein or polypeptide without regard to its structure or biological function by using a DNA segment coding for said protein or polypeptide as the mid-section of the tribrid protein.

Abstract: New anthracyclinones, including maggiemycin, anhydromaggiemycin and 7-oxidodaunorubicinone, have been prepared. Maggiemycin, anhydromaggiemycin, and 7-oxidodaunorubicinone all exhibit biological activity; they are toxic to cell lines KB, L1210 and P388.

Abstract: An anti-radial chromatographic device is disclosed preferably comprising an inner Petri dish nested concentrically within an outer Petri dish of slightly larger diameter. The bottom surface of the inner dish is spaced from the bottom surface of the outer dish to form a solvent-containing chamber. A annular wick contained in the annular space between the two dishes extends above the tops of the dishes and conducts the solvent upward to the lower surface of a downward-facing chromatographic plate resting on the wick.

Abstract: In an affinity detection apparatus wherein the specific recognition of a first chemical for a second chemical is used to identify the presence of either, and being of the type normally including a receptical tray having at least one well sized to hold both an insertable member and a solution containing the first of said chemicals, andAn insertable member coated with the second chemical, the improvement comprising:The coated member and well being complimentary shaped so as to define a first thin passageway between the member and well along substantially all the well periphery.