Abstract: Provided herein are controlled methods for producing biofuel and lipids from algae. In certain embodiments, the controlled methods comprise: (i) providing upwelled water in a body of water; (ii) culturing algae in the upwelled water; (iii) feeding the algae to planktivorious organisms; (iv) extracting lipids from the planktivorious organisms; and (v) polishing the lipids to make biofuel or other useful products. In certain embodiments, the upwelled water is provided in the body of water by using an open-cycle OTEC system. Also provided herein are controlled systems for producing biofuel and lipids from algae.

Abstract: The invention provides methods for producing biofuel from algae, that use fish which have a high capacity of producing and/or accumulating lipids to harvest algae from an algal culture. The invention also provides methods for growing fish that result in a high lipid content. The invention also provides methods for creating fish that have a high capacity of producing and accumulating lipids by breeding and/or recombinant DNA techniques. Also included are transgenic fish that have a higher lipid content than wild-type fish.

Abstract: Provided herein are systems and methods for producing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and/or derivatives and/or mixtures thereof by growing algae that produce the oils containing EPA and/or DHA and/or derivatives and/or mixtures thereof, harvesting the algae with fish in one or more enclosed systems, and then processing fish to separate and purify the EPA and/or DHA. The multi-trophic systems provided herein comprise at least one enclosure that contains the algae and the fishes, and means for controllably feeding the algae to the fishes. Also provided herein are the lipid compositions extracted from the fishes.

Abstract: Provided herein are systems and methods for extracting lipids and/or producing biofuel from algae in marine and freshwater environments, wherein algae and bivalves are co-cultured in a system of enclosures comprising water that comprises recycled nutrients that are essential for algal growth. The system also include enclosures for culturing fishes which are used to harvest the algae.

Abstract: Provided herein are systems and methods for extracting lipids and/or producing biofuel from algae in marine and freshwater environments, wherein algae and bivalves are co-cultured in a system of enclosures comprising water that comprises recycled nutrients that are essential for algal growth. The system also include enclosures for culturing fishes which are used to harvest the algae.

Abstract: Provided herein are systems and methods for producing ammonia fertilizer for culturing algae and aquaculture. The integrated systems provided herein can be installed on a hydrocarbon production platform and encompasses equipment and processes for generating electricity, preparation of an aqueous electrolyte, hydrogen generation by electrolysis, catalysis of ammonia formation, collection of ammonia, and optionally a nitrogen generator, and a plurality of subsystems for algae culture, and aquaculture.

Abstract: Disclosed herein are methods for mixing of carbon dioxide (CO2) and/or other nutrients in an ocean, wherein surface water CO2 is decreased to reduce or slow acidification of the ocean, and increases in fish populations are advantageously promoted. Also disclosed herein are methods and systems for recovery of nutrients, for example, phosphorus, from an ocean floor in the form of fish biomass, which may be used to make such useful products as fish fillets, fish meal, fish oil, biofuel or fertilizer.

Abstract: Provided herein are controlled methods for producing biofuel and lipids from algae. In certain embodiments, the controlled methods comprise: (i) providing upwelled water in a body of water; (ii) culturing algae in the upwelled water; (iii) feeding the algae to planktivorious organisms; (iv) extracting lipids from the planktivorious organisms; and (v) polishing the lipids to make biofuel or other useful products. In certain embodiments, the upwelled water is provided in the body of water by using an open-cycle OTEC system. Also provided herein are controlled systems for producing biofuel and lipids from algae.

Abstract: Disclosed herein are methods and systems for removing carbon dioxide (CO2) from water and quantifying the carbon so removed, thus facilitating valuation of that carbon for schemes (e.g., Kyoto agreement) that attach financial rewards for capture, sequestration or removal of carbon or CO2.

Abstract: Provided herein are systems and methods for sustainable aquaculture. The methods provided herein allows recovery and/or recycling of autochthonous nutrients in fish farming, and recovery of allochthonous nutrients present in eutrophic water. The systems provided herein comprises two closely spaced cages, an array of rotary panels that regulates the flow of matters between the two cages; and a means for producing a directional water current.

Abstract: Provided herein are systems and methods for producing biofuel from microalgae that use a population of zooplankton to harvest microalgae in a culture. The methods further comprise gathering the zooplankton, extracting lipids from the zooplankton, and processing the lipids to form biofuel. The systems provided herein comprise at least one enclosure comprising microalgae, means for making the microalgae available to a population of zooplankton, and means for gathering the zooplankton.

Abstract: The invention relates to systems and methods for reducing algal biomass in eutrophic water, wherein organism that feed on algae are introduced into the eutrophic water and cultured in the eutrophic water, until the algal biomass is reduced or the organisms have reach desirable size. The body of eutrophic water can be restocked with juveniles after harvesting. The organisms can be fishes and/or shellfishes. The methods further comprising producing biofuel, specialty chemicals, nutraceuticals, food, and/or fish meal from the harvested fish.

Abstract: The invention provides methods for producing biofuel from algae, that use fish which have a high capacity of producing and/or accumulating lipids to harvest algae from an algal culture. The invention also provides methods for growing fish that result in a high lipid content. The invention also provides methods for creating fish that have a high capacity of producing and accumulating lipids by breeding and/or recombinant DNA techniques. Also included are transgenic fish that have a higher lipid content than wild-type fish.

Abstract: Provided herein are systems and methods for producing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and/or derivatives and/or mixtures thereof by growing algae that produce the oils containing EPA and/or DHA and/or derivatives and/or mixtures thereof, harvesting the algae with fish in one or more enclosed systems, and then processing fish to separate and purify the EPA and/or DHA. The multi-trophic systems provided herein comprise at least one enclosure that contains the algae and the fishes, and means for controllably feeding the algae to the fishes. Also provided herein are the lipid compositions extracted from the fishes.

Abstract: The invention provides systems and methods for producing biofuel from algae wherein the algae and fishes are co-cultured in a body of water. The methods further comprise inducing the algae to accumulate lipids by environmental stress, and concentrating the algae prior to extraction of the algal oil. The systems of the invention comprise at least one growth enclosure, means for concentrating algae, and means for subjecting algae to environmental stress.

Abstract: The invention provides systems and methods for producing biofuel from algae that use cultured fish to harvest algae from an algal culture. The methods further comprise gathering the fish, extracting lipids from the fish, and processing the lipids to form biofuel. The multi-trophic systems of the invention comprises at least one enclosure that contains the algae and the fishes, and means for controllably feeding the algae to the fishes. The lipid compositions extracted from the fishes are also encompassed.

Abstract: The invention provides a system for obtaining biofuel from an algae composition comprising algae and water. The system comprises a pump for pressurizing the algae composition to a predefined pressure and a heater for heating the algae composition to a predefined temperature. Lipids in the algae are extracted and/or hydrolyzed to form fatty acids at a set of predefined temperature and pressure. The water may be in a subcritical or supercritical state at the predefined pressure and predefined temperature. The system further comprises a separator for partitioning the treated algae composition into an organic phase which includes the lipids and/or fatty acids, an aqueous phase, and a solid phase with biomass residue, and for collecting the organic phase. The organic phase can be upgraded to biofuel.