Patents Assigned to Mirus Corporation
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Patent number: 7049142Abstract: A process and compound wherein nucleic acids can be modified with a host of molecules and maintain their ability to be expressed. A modifying chemical attachment of polyions to polynucleotides can be used to facilitate the change of tertiary structure of the nucleic acid and in some cases condensation of nucleic acids into smaller, charged particles useful in delivering the nucleic acid to a cell.Type: GrantFiled: August 2, 2000Date of Patent: May 23, 2006Assignee: Mirus CorporationInventors: Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker
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Patent number: 6919091Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.Type: GrantFiled: March 11, 2002Date of Patent: July 19, 2005Assignee: Mirus CorporationInventors: Vladimir S. Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jon A. Wolff, David B. Rozema, Sean D. Monahan
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Patent number: 6818626Abstract: Chelator containing compounds are utilized in the delivery of molecules, polymers, nucleic acids and genes to animal cells. At least one chelator such as crown ether is attached to a polymer and then associated with another polymer such as DNA. An ion is then added to the mixture thereby forming condensed DNA. In condensed form and in complex with the chelator, DNA can be delivered to a cell.Type: GrantFiled: January 21, 1999Date of Patent: November 16, 2004Assignee: Mirus CorporationInventors: Jon A. Wolff, James E. Hagstrom, Sean D. Monahan, Vladimir Budker, Vladimir Trubetskoy, Paul M. Slattum
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Patent number: 6740336Abstract: We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.Type: GrantFiled: October 4, 2002Date of Patent: May 25, 2004Assignee: Mirus CorporationInventors: Vladimir S. Trubetskoy, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, So Chun Wong, Jason Klein
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Patent number: 6740643Abstract: The present invention relates to the delivery of desired compounds (e.g., nucleic acids) into cells using noncovalent delivery systems which include complexing nucleic acids, amphipathic binding agents, and amphiphiles.Type: GrantFiled: November 29, 2000Date of Patent: May 25, 2004Assignee: Mirus CorporationInventors: Jon A. Wolff, James E. Hagstrom, Sean D. Monahan, Vladimir Budker, David B. Rozema, Paul M. Slattum
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Patent number: 6706922Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.Type: GrantFiled: November 16, 2001Date of Patent: March 16, 2004Assignee: Mirus CorporationInventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson
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Patent number: 6673612Abstract: A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery.Type: GrantFiled: February 21, 2002Date of Patent: January 6, 2004Assignee: Mirus CorporationInventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker
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Patent number: 6630351Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.Type: GrantFiled: June 7, 2000Date of Patent: October 7, 2003Assignee: Mirus CorporationInventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
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Patent number: 6627616Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.Type: GrantFiled: November 23, 1999Date of Patent: September 30, 2003Assignee: Mirus CorporationInventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
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Patent number: 6593465Abstract: Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.Type: GrantFiled: January 23, 2001Date of Patent: July 15, 2003Assignee: Mirus CorporationInventors: Jon A. Wolff, James E. Hagstrom, Paul Michael Slattum, Vladimir Budker
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Patent number: 6458382Abstract: The present invention relates to compositions and methods for transferring nucleic acids into cells in vitro and in vivo. The compositions comprise a transfection reagent and one or more detergents. In preferred embodiments, the compositions comprise delivery systems providing nucleic acid transfer complexes that transfect cells with high efficiency.Type: GrantFiled: November 10, 2000Date of Patent: October 1, 2002Assignee: Mirus CorporationInventors: Hans Herweijer, Vladimir G. Budker
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Patent number: 6429200Abstract: A complex is described for delivery to a cell comprising inserting a nucleic acid into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery. Other molecules are used to interact with the nucleic acid—micelle complex to further enhance delivery such as a surfactant having a disulfide bond.Type: GrantFiled: July 16, 1999Date of Patent: August 6, 2002Assignee: Mirus CorporationInventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker
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Patent number: 6383811Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.Type: GrantFiled: January 2, 2001Date of Patent: May 7, 2002Assignee: Mirus CorporationInventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy
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Patent number: 6379966Abstract: Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.Type: GrantFiled: November 29, 1999Date of Patent: April 30, 2002Assignee: Mirus CorporationInventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
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Patent number: 6339067Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.Type: GrantFiled: December 30, 1997Date of Patent: January 15, 2002Assignee: Mirus CorporationInventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson
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Patent number: 6180784Abstract: The present invention provides a process of transfecting a cell with a polynucleotide mixed with one or more amphipathic compounds and an effective amount of a DNA-binding protein. Exemplary and preferred DNA-binding proteins are H1, H2A, and H2B. Exemplary and preferred amphipathic compounds are cationic amphipathic compounds.Type: GrantFiled: January 17, 1998Date of Patent: January 30, 2001Assignee: Mirus CorporationInventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Jeffery Fritz
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Patent number: 6126964Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.Type: GrantFiled: January 3, 1997Date of Patent: October 3, 2000Assignee: Mirus CorporationInventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson
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Patent number: 5744335Abstract: The present invention provides a process of transfecting a cell with a polynucleotide mixed with one or more amphipathic compounds and an effective amount of a DNA-binding protein. Exemplary and preferred DNA-binding proteins are H1, H2A, and H2B. Exemplary and preferred amphipathic compounds are cationic amphipathic compounds.Type: GrantFiled: September 19, 1995Date of Patent: April 28, 1998Assignee: Mirus CorporationInventors: Jon A. Wolff, Jeffery Fritz, Vladimir Budker, James Hagstrom
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Patent number: 4868677Abstract: An image recording system includes an electro-optic image section having a luminescent display device, such as a high-resolution monochrome cathode ray tube (CRT), that successively directs its light output through a red, a green, and blue filters to expose recording film contained within a camera. The recording film can take the form of positive roll film that is processed into slides or self-developing film units. The CRT is controlled to sweep a scan line at specified line positions while modulating the output light in a digital manner and repeat the line sweep until all pixel positions for that line are properly exposed. After all lines for a particular color are imaged, the filter is changed to the next color to be imaged and the process repeated until all scan lines for the different colors are completed. The system allows very precise exposure control to create developed images having fine gradation in color density and hue.Type: GrantFiled: January 13, 1988Date of Patent: September 19, 1989Assignee: Mirus CorporationInventor: Richard B. Tompane