Patents Assigned to Mixis France S.A.
  • Process for the reversible functional inactivation of the mismatch repair system
    Patent number: 7883894
    Abstract: The present invention relates to a process for allowing homologous recombination between non-identical DNA sequences of an organism and various applications thereof.
    Type: Grant
    Filed: December 19, 2002
    Date of Patent: February 8, 2011
    Assignee: Mixis France S.A.
    Inventors: Ivan Matic, Miroslav Radman
  • Generation of Recombinant Genes in Bacteriophages
    Publication number: 20100055669
    Abstract: In vivo methods for generating and detecting recombinant DNA sequences in bacteriophages or plasmids containing bacteriophage sequences, methods for generating hybrid genes and hybrid proteins encoded by these hybrid genes by the use of bacteriophages and plasmids containing bacteriophage sequences, bacteriophages and plasmids that can be used in these methods, and kits comprising appropriate bacterial host cells and bacteriophages or plasmids are described.
    Type: Application
    Filed: July 6, 2005
    Publication date: March 4, 2010
    Applicant: MIXIS FRANCE, S.A.
    Inventors: Alejandro Luque, Heike Strobel, Jann Thorsten Martinsohn, Marie-Agnès Petit, Miroslav Radman
  • Homologous recombination in mismatch repair inactivated eukaryotic cells
    Patent number: 7199280
    Abstract: A mammalian cell having a mismatch repair-deficient phenotype is provided, where one or both alleles of a gene essential for mismatch repair, such as an Msh gene, are inactivated. Using this cell in a gene knock-out methodology advantageously allows efficient homologous recombination, even when the DNA sequences of the donor and recipient sequences diverge by significantly more than 0.6%.
    Type: Grant
    Filed: June 20, 2001
    Date of Patent: April 3, 2007
    Assignee: Mixis France S.A.
    Inventors: Henricus Petrus Joseph Te Riele, Niels De Wind, Helena Maria Johanna Dekker-Vlaar
  • Meiotic recombination in vivo of partially homologous DNA sequences
    Patent number: 7115413
    Abstract: Process for the meiotic recombination in vivo of partially homologous DNA sequences having up to 30% of base mis-matches, wherein eukaryotic cells containing the sequences and in which an enzymatic mismatch repair system is defective, are maintained under conditions to effect meiosis. Preferably the enzymatic mismatch repair systems of the eukaryotic cells are defective by virtue of at least one mutS protein and/or at least one mutL protein being defective or missing. The eukaryotic cells may be unicellular organisms such as yeasts.
    Type: Grant
    Filed: March 27, 1997
    Date of Patent: October 3, 2006
    Assignee: Mixis France S.A.
    Inventors: Rhona Harriet Borts, Edward John Louis
  • Homologous recombination in mismatch repair inactivated eukaryotic cells
    Publication number: 20030221208
    Abstract: A mammalian cell having a mismatch repair-deficient phenotype is provided, where one or both alleles of a gene essential for mismatch repair, such as an Msh gene, are inactivated. Using this cell in a gene knock-out methodology advantageously allows efficient homologous recombination, even when the DNA sequences of the donor and recipient sequences diverge by significantly more than 0.6%.
    Type: Application
    Filed: February 12, 2003
    Publication date: November 27, 2003
    Applicant: Mixis France S.A.
    Inventors: Henricus Petrus Joseph Te Riele, Niels De Wind, Helena Maria Johanna Dekker-Vlaar
  • Process for the in vivo recombination of DNA sequences having mismatched bases
    Patent number: 5965415
    Abstract: The present invention relates to a process of recombination in vivo of partially homologous DNA sequences having up to 30% of base mismatches. According to its essential characteristic, said sequences are placed together in cells or an organism of which the enzymatic mismatch repair system is defective or has been transitorily inactivated by saturation for the time to obtain recombination between said DNA sequences or in using mutants which increase the intergeneric recombination.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: October 12, 1999
    Assignee: Mixis France, S.A.
    Inventors: Miroslav Radman, Christiane Rayssiguier
  • Process for the in vivo recombination of DNA sequences having mismatched bases
    Patent number: 5912119
    Abstract: The present invention relates to a process of recombination in vivo of partially homologous DNA sequences having up to 30% of base mismatches. According to its essential characteristic, said sequences are placed together in cells or an organism of which the enzymatic mismatch repair system is defective or has been transitorily inactivated by saturation for the time to obtain recombination between said DNA sequences or in using mutants which increase the intergeneric recombination.
    Type: Grant
    Filed: April 25, 1994
    Date of Patent: June 15, 1999
    Assignee: Mixis France, S.A.
    Inventors: Miroslav Radman, Christiane Rayssiguier