Abstract: The present invention provides a method for site-directed integration of DNA-sequences into the genome of plants via homologous recombination, by transforming said plants using the DNA-transfer system of Agrobacterium, in which the transforming DNA comprises in its most simple form a region homologous to the target locus, as well as a region which is different from the target locus either next to one or between two T-DNA borders.
Type:
Grant
Filed:
July 6, 1993
Date of Patent:
March 26, 1996
Assignee:
Mogen International, n.v./Rijksuniversiteit te Leiden
Inventors:
Remko Offringa, Marcellus J. A. De Groot, Paul J. J. Hooykaas, Petrus J. M. Van Den Elzen