Abstract: Disclosed are novel synthetically modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
Abstract: Compositions and methods for controlling insects by co-expressing an amino acid oxidase and a second enzyme that provides insecticidal activity when present in a mixture with the amino acid oxidase are disclosed. Also disclosed are DNA and protein sequences, and transformed microorganisms and plants useful for achieving such insect control.
Type:
Grant
Filed:
April 21, 1998
Date of Patent:
April 16, 2002
Assignee:
Monsanto Technolgy LLC
Inventors:
Barbara G. Isaac, John T. Greenplate, John P. Purcell, Charles P. Romano