Abstract: Conditioned media compositions having valuable biological activity are obtained from cultures of immune cells from elasmobranch fishes. Methods are provided for producing the conditioned media compositions. Conditioned media obtained using epigonal cells from bonnethead sharks (Sphyrna tiburo) and lemon sharks (Negaprion brevirostris) demonstrate strong anti-tumor activities. The conditioned media compositions can be used for treating tumor proliferation and in immunosuppressive therapy.

Abstract: The present invention includes a horizontal foam fractionation protein skimmer for removing organic waste material (dissolved organic compounds) from organic loaded water. The protein skimmer includes a horizontal reaction chamber connected to a foam collection chamber in which protein-loaded foam containing organic waste material is collected. The reaction chamber is comprised of a series of horizontally-directed, nested, concentric tubes for increasing the number and decreasing the size of bubbles to facilitate efficient removal of dissolved organic compounds. Each horizontal tube has either one or several small openings that are offset from the openings on the adjacent tube at a small angle, typically 10°. The openings are formed by either a single slit that runs along the length of each tube, or by a series of holes that runs along the length of each tube. Organic-loaded water is pumped through tubing into the reaction chamber. As water moves through the tubing, gas (e.g.