Abstract: The present disclosure provides a method for producing a cell-culturing polyvinyl alcohol-based nanofiber structure, the method comprising: electrospinning an electrospun solution to form a nanofiber mat, wherein the electrospun solution contains polyvinyl alcohol (PVA), polyacrylic acid (PA) and glutaraldehyde (GA); crosslinking the nanofiber mat via a hydrochloric acid (HCl) vapor treatment; and treating the crosslinked nanofiber mat with dimethylformamide (DMF) solvent to crystallize the nanofiber mat.

Abstract: Disclosed is a nanofiber-based long-term primary hepatocyte culture system and a culture method, wherein the primary hepatocyte culture system has an advantage that it can culture cells in three-dimensions in vitro to maintain the original physiological activity of low proliferative primary hepatocytes for a long time by co-culturing indirectly by separating primary hepatocytes and hepatic non-parenchymal cells with a support consisting of nanofibers therebetween without direct co-culture.

Abstract: A method of preparing a polyvinyl alcohol nanofiber membrane includes a material for controlling cell specific adhesion, and a nanofiber membrane that can maintain cellular functions such as cell activity and growth is prepared by adding aqueous solutions containing a polyacrylic acid and a glutaraldehyde crosslinking agent in a polyvinyl alcohol and materials capable of enhancing or regulating cell adhesion, electrospinning, treating with hydrochloric acid vapor and dimethylformaldehyde solvent and treating with sodium hydroxide to control the cell adhesion.

Abstract: The present disclosure provides a method for producing a cell-culturing polyvinyl alcohol-based nanofiber structure, the method comprising: electrospinning an electrospun solution to form a nanofiber mat, wherein the electrospun solution contains polyvinyl alcohol (PVA), polyacrylic acid (PA) and glutaraldehyde (GA); crosslinking the nanofiber mat via a hydrochloric acid (HCl) vapor treatment; and treating the crosslinked nanofiber mat with dimethylformamide (DMF) solvent to crystallize the nanofiber mat.

Abstract: A method of preparing a polyvinyl alcohol nanofiber membrane includes a material for controlling cell specific adhesion, and a nanofiber membrane that can maintain cellular functions such as cell activity and growth is prepared by adding aqueous solutions containing a polyacrylic acid and a glutaraldehyde crosslinking agent in a polyvinyl alcohol and materials capable of enhancing or regulating cell adhesion, electrospinning, treating with hydrochloric acid vapor and dimethylformaldehyde solvent and treating with sodium hydroxide to control the cell adhesion.