Abstract: A cell culture plate has a plurality of flow channels, uneven pattern areas and through holes. The flow channels are formed between the uneven pattern areas, and culture solution flows from the flow channels into the uneven pattern areas or from the uneven pattern areas into the flow channels. The uneven pattern area has uneven patterns that create a cell culture space.
Type:
Grant
Filed:
February 24, 2006
Date of Patent:
March 17, 2015
Assignees:
National Food Research Institute, Kuraray Co., Ltd.
Abstract: The present invention provides a method for detecting modified LDL, abnormal cells or bacteria using an intermolecular interaction analysis method, in which a region involved in ligand recognition by a receptor is expressed, without modification or as a biotinylated protein, in cells or in a test tube, and thereafter, the expressed region or the expressed biotinylated protein is immobilized via avidin or streptavidin to a solid phase while the orientation thereof is maintained, and the immobilized protein is utilized; and a kit for detecting the modified LDL or the like.
Abstract: It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
Type:
Grant
Filed:
October 26, 2010
Date of Patent:
January 7, 2014
Assignees:
National Food Research Institute, Riken
Abstract: It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
Type:
Grant
Filed:
May 26, 2005
Date of Patent:
December 10, 2013
Assignees:
National Food Research Institute, Riken
Abstract: Data collected by a transcranial brain function measuring/stimulating method is accurately projected and displayed onto a brain surface. If there is no three-dimensional head image, data is projected and displayed onto the brain surface of a standard brain. The head surface coordinates are transformed to the brain surface coordinates of the brain surface underlying the head surface by, e.g., a minimum distance search method. The coordinates of a projected point on the brain surface of the head surface and the probability distribution are determined for a standard brain normalized with data on subjects.
Type:
Grant
Filed:
November 12, 2004
Date of Patent:
May 15, 2012
Assignees:
Shimadzu Corporation, National Food Research Institute
Inventors:
Ippeita Dan, Kuniko Sakamoto, Masako Okamoto, Seiichiro Isobe, Kaoru Kohyama, Koji Shimizu, Kazuhiro Takeo
Abstract: It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
Type:
Application
Filed:
October 26, 2010
Publication date:
June 9, 2011
Applicants:
RIKEN, NATIONAL FOOD RESEARCH INSTITUTE
Abstract: The present invention provides a method for detecting modified LDL, abnormal cells or bacteria using an intermolecular interaction analysis method, in which a region involved in ligand recognition by a receptor is expressed, without modification or as a biotinylated protein, in cells or in a test tube, and thereafter, the expressed region or the expressed biotinylated protein is immobilized via avidin or streptavidin to a solid phase while the orientation thereof is maintained, and the immobilized protein is utilized; and a kit for detecting the modified LDL or the like.
Abstract: The present invention relate to a method of detecting a genetic recombinant by using the PCR method. A method of quantitatively detecting method is provided whereby the total content ratio of the genetic recombinants and the individual content ratio of the genetic recombinant in a population containing plural genetic recombinant lines can be quantified. The method of the present invention comprises performing PCR for the DNA sequence specific to the recombinant and the endogenous DNA sequence shared by the species corresponding to the recombinant using, as a standard molecule, a molecule containing the DNA sequence specific to the recombinant and the endogenous DNA sequence shared by the species on the single molecule, and determining the content ratio of the number of molecules thereof.
Type:
Grant
Filed:
April 25, 2003
Date of Patent:
May 4, 2010
Assignees:
National Food Research Institute, Asahi Breweries, Ltd.
Abstract: This invention relates to a method for increasing the productivity of the secondary metabolite in a micro-organism, for example, the improvement of antibiotic producers by conferring drug-resistant mutations to micro-organisms.
Type:
Grant
Filed:
March 29, 2002
Date of Patent:
January 1, 2008
Assignees:
The National Food Research Institute, Astellas Pharma Inc.
Abstract: Data collected by a transcranial brain function measuring/stimulating method is accurately projected and displayed onto a brain surface. If there is no three-dimensional head image, data is projected and displayed onto the brain surface of a standard brain. The head surface coordinates are transformed to the brain surface coordinates of the brain surface underlying the head surface by, e.g., a minimum distance search method. The coordinates of a projected point on the brain surface of the head surface and the probability distribution are determined for a standard brain normalized with data on subjects.
Type:
Application
Filed:
November 12, 2004
Publication date:
December 6, 2007
Applicants:
SHIMADZU CORPORATION, NATIONAL FOOD RESEARCH INSTITUTE
Inventors:
Ippeita Dan, Kuniko Sakamoto, Masako Okamoto, Seiichiro Isobe, Kaoru Kohyama, Koji Shimizu, Kazubiro Takeo
Abstract: A cell culture plate has a plurality of flow channels, uneven pattern areas and through holes. The flow channels are formed between the uneven pattern areas, and culture solution flows from the flow channels into the uneven pattern areas or from the uneven pattern areas into the flow channels. The uneven pattern area has uneven patterns that create a cell culture space.
Type:
Application
Filed:
February 24, 2006
Publication date:
August 30, 2007
Applicants:
National Food Research Institute, Kuraray Co., Ltd.
Abstract: A resin microchannel array includes a first substrate having a plurality of depressions, each depression having an inlet port at one end and an outlet port at another end, and walls sectioning the depressions, each wall having a micro groove connecting the depressions, and a second substrate having a flat surface bonded or pressure-contacted to a surface of the first substrate. Spaces created by the depressions and the grooves in a bonded or pressure contacted part between the first substrate and the second substrate serve as flow channels. Each of a width and a depth of the flow channel is within a range of 1 to 50 ?m, and a ratio of the width and the depth of the flow channel is within a range of 1:10 to 10:1.
Type:
Application
Filed:
February 24, 2006
Publication date:
August 30, 2007
Applicants:
National Food Research Institute, Kuraray Co., Ltd.
Abstract: Provided is a DNA-level grain variety discrimination method of detecting the presence or absence of any other varieties of grains in object grains of a certain variety through multiplex PCR that uses the DNAs extracted from the grains or from their processed products as templates. The method is characterized in that the multiplex PCR uses pair primer groups that are for discriminative detection of negative bands not appearing in the band pattern of the object variety but selectively appearing only in the band patterns of the other mixed varieties. The method has made it possible to rapidly and simply detect the presence or absence of mixed varieties in high-quality grains such as “Koshihikari”, and to identify the mixed varieties.
Type:
Application
Filed:
December 30, 2005
Publication date:
August 17, 2006
Applicants:
National Food Research Institute, Takara Bio Inc.
Abstract: The present invention relates to mutant strains of Pseudomonas putida and their use in the detoxification of industrial and/or toxic waste products.
Abstract: Provided is a DNA-level grain variety discrimination method of detecting the presence or absence of any other varieties of grains in object grains of a certain variety through multiplex PCR that uses the DNAs extracted from the grains or from their processed products as templates. The method is characterized in that the multiplex PCR uses pair primer groups that are for discriminative detection of negative bands not appearing in the band pattern of the object variety but selectively appearing only in the band patterns of the other mixed varieties. The method has made it possible to rapidly and simply detect the presence or absence of mixed varieties in high-quality grains such as “Koshihikari”, and to identify the mixed varieties.
Type:
Grant
Filed:
August 13, 2002
Date of Patent:
May 9, 2006
Assignees:
National Food Research Institute, Takara Bio Inc.
Abstract: It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation as conferring a resistance or dependence to streptomycin on E. coli whereby the reading efficiency of mRNA codon on ribosomes can be improved and thus the protein productivity can be elevated.
Type:
Application
Filed:
May 26, 2005
Publication date:
January 12, 2006
Applicants:
RIKEN, NATIONAL FOOD RESEARCH INSTITUTE
Abstract: Protein having an erythrose reductase activity; a DNA encoding the protein; a cell to which a DNA has been transferred in a manner such that the DNA is capable of expressing an erythrose reductases type III, II or I the DNA encodes; and a method for producing erythritol, comprising acting the erythrose reductases type III, II or I or a cell to which erythrose reductases type III, II or I has been transferred in a manner capable of expressing the erythrose reductases type III, II or I on D-erythrose and harvesting the produced erythritol.
Type:
Grant
Filed:
January 9, 2002
Date of Patent:
November 1, 2005
Assignee:
Director of National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
Abstract: Protein having an erythrose reductase activity; a DNA encoding the protein; a cell to which a DNA has been transferred in a manner such that the DNA is capable of expressing an erythrose reductases type III, II or I the DNA encodes; and a method for producing erythritol, comprising acting the erythrose reductases type III, II or I or a cell to which erythrose reductases type III, II or I has been transferred in a manner capable of expressing the erythrose reductases type III, II or I on D-erythrose and harvesting the produced erythritol.
Type:
Application
Filed:
May 10, 2005
Publication date:
October 20, 2005
Applicant:
Director of National Food Research Institute
Abstract: The resin microchannel substrate has a surface where a recess leading to a fluid supply port and a bank adjacent to the recess and having many micro grooves on a surface are formed. The grooves form microchannels connecting the inside of the recess and the outside of the recess when the surface of the substrate is firmly attached to a flat plate serving as a cover. The width and height of the microchannel are within a range of 1 to 300 ?m, and the width/height ratio of the channel is within a range of 1:20 to 20:1.
Type:
Application
Filed:
April 1, 2004
Publication date:
August 4, 2005
Applicants:
National Food Research Institute, KURARAY CO. LTD.
Abstract: A technique has been developed for degrading toxic substances such as aflatoxins-related substances, without affecting on the properties of agricultural commodities, foods and feeds. The technique includes a composition for degrading toxic substances selected from aflatoxins-related substances and toxic aromatic compounds, the composition which contains a hemin and/or heme-containing substance, and a method for detoxifying agricultural commodities, foods or feeds contaminated with toxic substances selected from aflatoxins-related substances and toxic aromatic compounds, the method which comprises contacting the contaminated agricultural commodities, foods or feeds with a hemin and/or heme-containing substance.