Abstract: A fusion protein according to an embodiment of the present disclosure includes a growth differentiation factor 11 and an epidermal growth factor with an enhanced anti-oxidation activity and an enhanced skin cell proliferation effect, A cosmetic composition according to an embodiment of the present disclosure includes the fusion protein as an effective component. The cosmetic composition can be advantageously used in future as a material of a functional cosmetic product.
Type:
Grant
Filed:
June 1, 2017
Date of Patent:
May 21, 2024
Assignees:
NEXGEN BIOTECHNOLOGIES, INC.
Inventors:
Sun Kyo Lee, Seong Ran Lee, Han Bong Ryu, Tae Hyun Kim
Abstract: A fusion protein includes heat shock protein 10 and brazzein protein. The fusion protein has an enhanced anti-oxidation activity and skin cell proliferation effect. It can be used as a cosmetic composition for ameliorating skin wrinkles. The cosmetic composition including the fusion protein can be advantageously used in future as a material of a functional cosmetic product.
Type:
Grant
Filed:
June 1, 2017
Date of Patent:
October 17, 2023
Assignees:
NEXGEN BIOTECHNOLOGIES, INC.
Inventors:
Sun Kyo Lee, Seong Ran Lee, Han Bong Ryu, Tae Hyun Kim, Tae Won Choi
Abstract: A human growth hormone fusion protein has enhanced thermal stability. The human growth hormone fusion protein has the amino acid sequence SEQ ID NO: 3 or SEQ ID NO: 4. A gene encoding the human growth hormone fusion protein has E. coli codon-optimized nucleotide sequence of SEQ ID NO: 5 or SEQ ID NO: 6. A recombinant vector includes the aforementioned gene. A host cell is transformed with the aforementioned recombinant vector. A method for producing in a host cell a human growth hormone fusion protein includes transforming a host cell with the aforementioned recombinant vector. The human growth hormone fusion protein may improve skin wrinkle and maintain skin elasticity. As the human growth hormone fusion protein has thermal stability and also has an effect of improving skin wrinkle and increasing the effect of maintaining skin elasticity, it can be advantageously used as a raw material of cosmetics.
Type:
Grant
Filed:
January 9, 2017
Date of Patent:
November 9, 2021
Assignees:
NEXGEN BIOTECHNOLOGIES, INC.
Inventors:
Sun Kyo Lee, Seong Ran Lee, Han Bong Ryu, Tae Won Choi, Tae Hyun Kim
Abstract: A fusion protein of heat shock proteins has an increased anti-oxidant activity and skin cell proliferation effect. An anti-wrinkle composition which has the fusion protein as an effective component can be advantageously used as a material of a functional cosmetic product. The fusion protein may be composed of heat shock protein 10 and heat shock protein 20. The fusion protein may include the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4.
Type:
Application
Filed:
August 1, 2017
Publication date:
April 8, 2021
Applicants:
NEXGEN BIOTECHNOLOGIES, INC.
Inventors:
Sun Kyo LEE, Seong Ran LEE, Han Bong RYU, Tae Hyun KIM, Tae Won CHOI
Abstract: A fusion protein has the amino acid sequence of SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8. A composition having the fusion protein may improve skin wrinkle with increased anti-oxidant activity and skin cell proliferation effect. It can be advantageously used as a raw material of a functional cosmetic having an excellent skin regeneration effect like improvement of skin wrinkles and skin whitening.
Type:
Grant
Filed:
August 31, 2017
Date of Patent:
October 27, 2020
Assignees:
NEXGEN BIOTECHNOLOGIES, INC.
Inventors:
Sun Kyo Lee, Tae Hyun Kim, Seong Ran Lee, Han Bong Ryu, Tae Won Choi, Hyeong Il Kwon, Woo Yeon Roh
Abstract: A fusion protein having the amino acid sequence of SEQ ID NO: 3 or SEQ ID NO: 4 has enhanced skin cell proliferation effect. A composition includes, as an effective ingredient, the fusion protein for improving skin wrinkle, skin elasticity, and anti-aging. Because the composition has an excellent cell proliferation effect and an effect of improving skin wrinkle and enhancing skin elasticity, it is expected to be used advantageously in the field of functional cosmetics.
Abstract: A method for treating wrinkle or aging on a skin of a subject includes applying to the skin a composition that includes a green fluorescent protein-human epidermal growth factor fusion protein as an effective component. Since the fusion protein has an excellent anti-oxidation activity and a high skin cell proliferation effect, it can be advantageously used as a raw material of functional cosmetics having excellent skin regeneration effect like improvement of skin wrinkles and skin whitening.
Type:
Grant
Filed:
January 25, 2017
Date of Patent:
October 15, 2019
Assignees:
NEXGEN BIOTECHNOLOGIES, INC.
Inventors:
Sun Kyo Lee, Seong Ran Lee, Han Bong Ryu, Tae Hyun Kim, Tae Won Choi, Hyeong Il Kwon
Abstract: A botulinum toxin-human epidermal growth factor fusion protein with increased skin cell proliferation and anti-oxidation effect has the amino acid sequence of SEQ ID NO: 2, a gene consisting of E. coli (Escherichia coli) codon-optimized nucleotide sequence of SEQ ID NO: 1 for encoding the botulinum toxin-human epidermal growth factor fusion protein, a recombinant vector including the gene, a host cell transformed with the recombinant vector, and a method for producing a botulinum toxin-human epidermal growth factor fusion protein by transforming a host cell with the recombinant vector, and a cosmetic composition for regenerating skin and improving skin wrinkle including, as an effective component, a botulinum toxin-human epidermal growth factor fusion protein, and as the cosmetic composition has an effect of regenerating skin and improving skin wrinkle, it can be advantageously used in future in the field of cosmetics or cosmetic plastic surgery.
Type:
Grant
Filed:
July 22, 2016
Date of Patent:
April 23, 2019
Assignees:
NEXGEN BIOTECHNOLOGIES, INC.
Inventors:
Sun Kyo Lee, Han Bong Ryu, Seong Ran Lee, Jong Nam Choi, Tae Won Choi, Tae Hyun Kim, Tae Hwa Jeong, Hyeong Il Kwon
Abstract: A method for treating wrinkle or aging on a skin of a subject includes applying to the skin a composition that includes a green fluorescent protein-human epidermal growth factor fusion protein as an effective component. Since the fusion protein has an excellent anti-oxidation activity and a high skin cell proliferation effect, it can be advantageously used as a raw material of functional cosmetics having excellent skin regeneration effect like improvement of skin wrinkles and skin whitening.
Type:
Application
Filed:
January 25, 2017
Publication date:
January 24, 2019
Applicant:
Nexgen Biotechnologies, Inc.
Inventors:
Sun Kyo LEE, Seong Ran LEE, Han Bong RYU, Tae Hyun KIM, Tae Won CHOI, Hyeong Il KWON
Abstract: A thermostable human epidermal growth factor-spider venom fusion protein with increased skin cell proliferation effect has the amino acid sequence of SEQ ID NO: 2, a gene of E. coli codon-optimized nucleotide sequence of SEQ ID NO: 1 for encoding the human epidermal growth factor-spider venom fusion protein, a recombinant vector including the gene, a host cell transformed with the recombinant vector, and a method for producing in a host cell a human epidermal growth factor-spider venom fusion protein by transforming a host cell with the recombinant vector, and a cosmetic composition for improving skin wrinkle and maintaining skin elasticity including a human epidermal growth factor-spider venom fusion protein, and as the cosmetic composition has excellent thermostability and has an effect of enhancing the activity of improving skin wrinkle and maintaining skin elasticity, it can be advantageously used in future in the field of cosmetics or cosmetic plastic surgery.
Type:
Grant
Filed:
July 22, 2016
Date of Patent:
November 13, 2018
Assignees:
NEXGEN BIOTECHNOLOGIES, INC
Inventors:
Sun Kyo Lee, Han Bong Ryu, Seong Ran Lee, Jong Nam Choi, Tae Hyun Kim, Tae Won Choi, Tae Hwa Jeong, Hyeong Il Kwon
Abstract: A spider venom (SV) 82 polypeptide has an amino acid sequence of SEQ ID NO: 2. An SV82 polypeptide-encoding gene having a nucleotide sequence of SEQ ID NO: 1 which is optimized for E. coli codon. A recombinant vector includes the SV82 polypeptide-encoding gene, An E. coli is transformed with the recombinant vector. A method of producing SV82 polypeptide includes transforming a host cell with the recombinant vector. A cosmetic composition for reducing skin wrinkles and maintaining skin elasticity includes SV82 polypeptide as an active ingredient.
Type:
Grant
Filed:
August 26, 2016
Date of Patent:
January 30, 2018
Assignee:
NEXGEN BIOTECHNOLOGIES, INC.
Inventors:
Sun Kyo Lee, Je Geun Yoo, Seong Ran Lee, Han Bong Ryu, Tae Won Choi, Jong Nam Choi, Tae Hyun Kim