Abstract: A treatment method for preservation of plant leaves wherein the plant leaves are immersed in a dehydrating solvent consisting of acetone and ethyl alcohol to replace the tissue water and remove chlorophyll in the leaves by the dehydrating solvent. Thereafter, the leaves are immersed in a permeating solution containing polyethylene glycol and acetone for allowing polyethylene glycol to permeate the leaves for replacing the dehydrating solvent. Thereafter, the leaves are dyed with a coloring matter.