Abstract: The invention is based on the use of a basic reagent under basic conditions to separate conjugated saccharide from unconjugated components in a sample, e.g. a vaccine, by precipitation of the conjugated saccharide. The invention allows rapid and quantitative separation of conjugated and conjugated components, which may be exploited in analytical methods for quantifying unconjugated saccharide or carrier. Therefore, the separation of conjugated and unconjugated components using the invention may be advantageously combined with a quantitative saccharide or carrier analysis to provide improved quality control for conjugate vaccines.
Type:
Application
Filed:
March 21, 2007
Publication date:
July 9, 2009
Applicant:
NOAVARTIS VACCINES AND DIAGNOSTICS SRL
Inventors:
Francesco Berti, Bruno Galletti, Pierino Parente, Paolo Costantino