Abstract: A process for creating acellular donor soft tissue by removing contaminants such as cells by extraction using a fluid at supercritical temperature and pressures while preserving the integrity of the soft tissue. The soft tissue is maintained in contact with the fluid in a pressure vessel at supercritical temperature and pressures for a period of time and then the pressure vessel is rapidly depressurized.

Abstract: A method for sterilizing a ground meat product through the application supercritical CO2 during the processing of the meat by introducing supercritical CO2 into an extruder as the meat passes through the extruder while controlling the pressure and speed at which the meat passes through the extruder to maintain the meat in contact with the supercritical CO2 for a time sufficient achieve a log reduction in colony forming units of microbial contaminants.

Abstract: Treating absorbable sutures that have been sterilized using ethylene oxide with carbon dioxide at or near its supercritical pressure and temperature conditions to remove any residual ethylene oxide.

Abstract: Whole organisms are inactivated by at least a factor of 106 using carbon dioxide at or near its supercritical pressure and temperature conditions.

Abstract: Whole organisms are inactivated by at least a factor of 106 using carbon dioxide at or near its supercritical pressure and temperature conditions.

Abstract: A method is disclosed that produces allografts from matrices typically containing demineralized bone matrix (DBM) powder, demineralized bone matrix gel, demineralized bone matrix paste, bone cement, cancellous bone, or cortical bone and mixtures thereof. The matrices are sterilized utilizing supercritical CO2 in the presence of a sterilizing additive and an entrainer such as an alkaline earth metal compound, preferably CaCO3. The resultant allograft materials have a reduced rate of rejection when used in allograft procedures including, bone, cartilage, tendon, and ligament grafting procedures.

Abstract: Sterilization methods and apparatus are effective to achieve a 6-log reduction in CFUs of industry standard bacteria and bacterial spores, i.e., B. stearothermophilus and B. subtilis spores, by subjecting sterilizable materials to a chemical additive-containing carbon dioxide sterilant fluid at or near its supercritical pressure and temperature conditions. Most preferably, the chemical additive-containing supercritical carbon dioxide sterilant fluid is agitated during sterilization, e.g., via mechanical agitation or via pressure cycling.

Abstract: Sterilization methods and apparatus are effective to achieve a 6-log reduction in CFUs of industry standard bacteria and bacterial spores, i.e., B. stearothermophilus and B. subtilis spores, by subjecting sterilizable materials to a chemical additive-containing carbon dioxide sterilant fluid at or near its supercritical pressure and temperature conditions. Most preferably, the chemical additive-containing supercritical carbon dioxide sterilant fluid is agitated during sterilization, e.g., via mechanical agitation or via pressure cycling.