Patents Assigned to NOVOGY, INC.
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Patent number: 10760105Abstract: Disclosed are transformed cells comprising one or more genetic modifications that increase the lipid content of the cell, e.g., relative to an unmodified cell of the same type. Also disclosed are methods for increasing the lipid content of a cell by increasing the activity of one or more proteins in the cell and/or by decreasing the activity of one or more proteins in the cell.Type: GrantFiled: December 29, 2015Date of Patent: September 1, 2020Assignee: NOVOGY, INC.Inventors: Arthur J. Shaw, IV, Johannes Pieter Van Dijken, Annapurna Kamineni, Jonathan Friedlander, Vasiliki Tsakraklides, Maureen Hamilton, Elena E. Brevnova
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Patent number: 10724041Abstract: Disclosed are nucleotide sequences and corresponding amino acid sequences of Arxula adeninivorans genes that can be utilized to manipulate the lipid content and/or composition of a cell. Methods and compositions for utilizing this information are disclosed to increase the lipid content or modify the lipid composition of a cell by either increasing or decreasing the activity of certain genetic targets.Type: GrantFiled: May 29, 2015Date of Patent: July 28, 2020Assignee: NOVOGY, INC.Inventors: Elena E. Brevnova, Arthur J. Shaw, IV, Johannes P. Van Dijken
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Patent number: 10457963Abstract: Nucleic acids and cells comprising a methyltransferase gene and/or a reductase gene are disclosed. These nucleic acids and cells may be used to produce branched (methyl)lipids, such as 10-methylstearate.Type: GrantFiled: September 20, 2017Date of Patent: October 29, 2019Assignee: Novogy, Inc.Inventors: Arthur J. Shaw, IV, Hannah Blitzblau, Donald V. Crabtree
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Patent number: 10443047Abstract: Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by up-regulating diacylglycerol acyltransferase and down-regulating triacylglycerol lipase. In some embodiments, a DGA1 protein is expressed and a native TGL3 gene is knocked out, thereby increasing the synthesis of triacylglycerol and decreasing its consumption, respectively.Type: GrantFiled: May 1, 2015Date of Patent: October 15, 2019Assignee: Novogy, Inc.Inventors: Vasiliki Tsakraklides, Elena E. Brevnova
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Patent number: 10316323Abstract: Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.Type: GrantFiled: August 17, 2017Date of Patent: June 11, 2019Assignee: Novogy, Inc.Inventors: Colin South, Arthur J Shaw, IV
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Patent number: 10260077Abstract: Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by increasing the activity of a type 1 diacylglycerol acyltransferase (i.e., DGA2) and increasing the activity of a type 2 diacylglycerol acyltransferase (i.e., DGA1). In some embodiments, the triacylglycerol content of a cell is also modified by decreasing the activity of a triacylglycerol lipase in the same cell. Also disclosed are methods and compositions for increasing the triacylglycerol content of a cell by increasing the activity of a type 1 diacylglycerol acyltransferase (i.e., DGA2), or by increasing the activity of a type 3 diacylglycerol acyltransferase (i.e., DGA3).Type: GrantFiled: May 29, 2015Date of Patent: April 16, 2019Assignee: Novogy, Inc.Inventors: Vasiliki Tsakraklides, Elena E. Brevnova
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Patent number: 10260052Abstract: DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinium, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.Type: GrantFiled: February 24, 2015Date of Patent: April 16, 2019Assignee: NOVOGY, INC.Inventors: Elena E. Brevnova, Arthur J. Shaw, IV, Emily H. Greenhagen
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Patent number: 10174296Abstract: Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical phosphorus or sulfur sources. Fermentation methods using the genetically engineered organisms are also described. The fermentation methods are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.Type: GrantFiled: August 27, 2014Date of Patent: January 8, 2019Assignee: Novogy, Inc.Inventors: Arthur J. Shaw, IV, Colin R. South, Johannes P. Van Dijken
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Patent number: 9765348Abstract: Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.Type: GrantFiled: January 6, 2014Date of Patent: September 19, 2017Assignee: Novogy, Inc.Inventors: Colin R. South, Arthur J. Shaw, IV
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Publication number: 20170204401Abstract: Disclosed are methods and compositions for reducing horizontal gene transfer of functional proteins. In some embodiments, the risk of horizontal gene transfer of a functional protein is reduced by separately encoding domains of a protein on at least two spatially distinct nucleic acid sequences, where each individual domain alone is non-functional, but co-expression of the encoded domains results in their association to form a functional protein.Type: ApplicationFiled: March 3, 2015Publication date: July 20, 2017Applicant: Novogy, Inc.Inventors: Elena E. Brevnova, Emily H. Greenhagen, Arthur J. Shaw, IV, Colin R. South
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Publication number: 20170096691Abstract: Disclosed are transformed cells and related nucleotide and protein sequences, and fermentation compositions and methods, all of which are related to providing selective advantage in fermentation. For example, a selective advantage results from transformation of a cell with a nucleic acid that allows a transformed cell to metabolize one or more nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell cannot metabolize, and from fermentation of the transformed cell using one or more feedstocks, such as fractioned grain, which are depleted in or free of conventional nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell can metabolize. Also disclosed are methods for improved oxygen transfer in an aerobic or microaerobic fermentation.Type: ApplicationFiled: April 8, 2015Publication date: April 6, 2017Applicant: Novogy, Inc.Inventors: Emily H. Greenhagen, Maureen Hamilton, William G. LaTouf, Andrew L. Consiglio, Kyle M. MacEwen, Arthur J. Shaw, IV
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Publication number: 20160362663Abstract: DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinium, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.Type: ApplicationFiled: February 24, 2015Publication date: December 15, 2016Applicant: NOVOGY, INC.Inventors: Elena E. Brevnova, Arthur J. Shaw, Emily H. Greenhagen
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Publication number: 20160115492Abstract: Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.Type: ApplicationFiled: January 6, 2014Publication date: April 28, 2016Applicant: NOVOGY, INC.Inventors: Colin R. South, Arthur J. Shaw, IV