Abstract: Provided herein compositions and methods for producing isoprenoids, including squalene. In certain aspects and embodiments provided are genetically altered yeast and uses therefore. In some aspects and embodiments, the genetically altered yeast produce isoprenoids, preferably squalene. The genetically altered yeast may have alterations in the expression or activity of enzymes involved in squalene production, for example, acetyl-CoA carboxylase (or “ACCase”), HMG-CoA reductase, squalene epoxidase, and squalene synthase. One or more genes of a genetically altered yeast may be modified by gene repair oligonucleobases. Also are provided methods of producing squalene using a genetically altered yeast. The invention also provides squalene produced by genetically altered yeast.
Type:
Grant
Filed:
May 22, 2009
Date of Patent:
October 16, 2018
Assignee:
NUCELIS INC.
Inventors:
Keith A. Walker, Mark E. Knuth, Noel M. Fong, Peter R. Beetham
Abstract: Provided herein are blends oils or fatty acids comprising more than 50% medium chain fatty acids, or the fatty acid alkyl esters thereof, and having low melting points. Such blends are useful as a fuel or as a starting material for the production of, for example, a biodiesel. Also provided genetically altered or modified plants, modified such that the amount of medium chain fatty acids generated by the plant are increased. Further provided is a method of predicting the melting point of a blend of fatty acid methyl esters and the use of such a method for identifying blends suitable for use as, for example, a biodiesel.
Type:
Grant
Filed:
January 28, 2013
Date of Patent:
October 24, 2017
Assignee:
NUCELIS INC.
Inventors:
Mark Knuth, Peter R. Beetham, Keith Walker, Greg F. W. Gocal
Abstract: Provided herein compositions and methods for producing isoprenoids, including squalene. In certain aspects and embodiments provided are genetically converted yeast and uses therefore. In some aspects and embodiments, the genetically converted yeast produce isoprenoids, preferably squalene. Also are provided methods of producing squalene using a genetically converted yeast or a non-genetically converted yeast. The invention also provides squalene produced by genetically converted yeast or non-genetically converted yeast.
Type:
Grant
Filed:
November 22, 2010
Date of Patent:
June 25, 2013
Assignee:
Nucelis Inc.
Inventors:
Keith A. Walker, Mark E. Knuth, Noel M. Fong, Peter R. Beetham
Abstract: Provided herein are blends oils or fatty acids comprising more than 50% medium chain fatty acids, or the fatty acid alkyl esters thereof, and having low melting points. Such blends are useful as a fuel or as a starting material for the production of, for example, a biodiesel. Also provided genetically altered or modified plants, modified such that the amount of medium chain fatty acids generated by the plant are increased. Further provided is a method of predicting the melting point of a blend of fatty acid methyl esters and the use of such a method for identifying blends suitable for use as, for example, a biodiesel.
Type:
Grant
Filed:
September 7, 2011
Date of Patent:
January 29, 2013
Assignee:
Nucelis Inc.
Inventors:
Mark Knuth, Peter R. Beetham, Keith Walker, Greg F. W. Gocal