Patents Assigned to Nuclera Ltd
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Patent number: 11946901Abstract: A method for degassing a microfluidic droplet by combining electrowetting and heating to induce formation of gaseous bubbles in the droplet. In an embodiment the methods are carried out on an active matrix of electrowetting electrodes including a hydrophobic coating. A carrier fluid is flowed against the droplet motion propelled by electrowetting to facilitate rapid removal of the gasses departing the droplet.Type: GrantFiled: January 25, 2021Date of Patent: April 2, 2024Assignee: Nuclera LTDInventors: Tanya Gupta, Luke M. Slominski, David Zhitomirsky, Richard J. Paolini, Jr.
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Publication number: 20240084350Abstract: The invention relates to the use of specific terminal deoxynucleotidyl transferase (TdT) enzymes in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of terminal deoxynucleotidyl transferases and 3?-blocked nucleotide triphosphates in a method of template independent nucleic acid synthesis.Type: ApplicationFiled: September 21, 2023Publication date: March 14, 2024Applicant: Nuclera Ltd.Inventors: Michael C. Chen, Radu A. Lazar, Jiahao Hung, Gordon R. Mclnroy
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Patent number: 11927740Abstract: An active matrix electrowetting on dielectric (AM-EWoD) device including a substrate with thin-film transistors (TFT), a dielectric layer, and a spatially variable wettability layer covering the dielectric layer. As depicted herein, the spatially variable wettability layer may include a plurality of portions having different contact angles, one or more contact angle gradients, or both.Type: GrantFiled: November 19, 2020Date of Patent: March 12, 2024Assignee: Nuclera LTDInventor: David Zhitomirsky
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Patent number: 11921394Abstract: Layered dielectric materials for use in controlling dielectric strength in microelectronic devices, especially as they relate to electrophoretic and electrowetting applications. Specifically, a combination of a first atomic layer deposition (ALD) step, a sputtering step, and a second ALD step result in a layer that is chemically robust and nearly pinhole free. The dielectric layer may be disposed on the transparent common electrode of an electrophoretic display or covering the pixelated backplane electrodes, or both.Type: GrantFiled: August 15, 2022Date of Patent: March 5, 2024Assignee: Nuclera LTDInventor: Cristina Visani
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Patent number: 11821018Abstract: Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.Type: GrantFiled: October 19, 2022Date of Patent: November 21, 2023Assignee: Nuclera Ltd.Inventors: Michael Chun Hao Chen, Sihong Chen, Christopher Dulson, Sumit Kalsi, Gordon Ross McInroy, Stephanie Reikine
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Patent number: 11807887Abstract: The invention relates to the use of specific terminal deoxynucleotidyl transferase (TdT) enzymes in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of terminal deoxynucleotidyl transferases and 3?-blocked nucleotide triphosphates in a method of template independent nucleic acid synthesis.Type: GrantFiled: December 17, 2021Date of Patent: November 7, 2023Assignee: Nuclera, Ltd.Inventors: Michael C. Chen, Radu A. Lazar, Jiahao Huang, Gordon R. McInroy
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Patent number: 11806715Abstract: A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the confornial layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the niatrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.Type: GrantFiled: October 13, 2022Date of Patent: November 7, 2023Assignee: Nuclera LtdInventors: Michael Chun Hao Chen, Sumit Kalsi, Laurence Livingstone Bell, Gordon Ross McInroy, David Zhitomirsky, Luke M. Slominski, Richard J. Paolini, Jr., Cristina Visani
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Patent number: 11801510Abstract: An electrowetting system is disclosed. The system includes electrodes configured to manipulate droplets of fluid in a microfluidic space. Each electrode is coupled to circuitry operative to selectively apply a driving voltage to the electrode. The system includes a dielectric stack including a first dielectric pair comprising a first layer having a first dielectric constant and a second layer having a second dielectric constant. The second dielectric constant is larger than the first dielectric constant. The dielectric stack includes a second dielectric pair comprising a third layer having a third dielectric constant and a fourth layer having a fourth dielectric constant. The fourth dielectric constant is larger than the third dielectric constant. A ratio of a thickness of the fourth layer to a thickness of the third layer (T4:T3) is in the range from about 2:1 to about 8:1. The second dielectric pair is thinner than the first dielectric pair.Type: GrantFiled: December 16, 2022Date of Patent: October 31, 2023Assignee: Nuclera LtdInventors: Cristina Visani, Richard J. Paolini, Jr.