Abstract: The present application relates to a method for the cytometric analysis of multiple cell samples by a microscope for examining multiple cell samples under a microscope, wherein the microscope can be or is operated, selectively and/or alternatingly, in a transmission mode and/or in a fluorescence mode, and wherein at least one cell sample has at least one fluorescence marker. The method includes; moving the cell samples continuously in one plane relative to an optical system of the microscope having at least one microscope camera, wherein, during the movement of the cell samples, at least one or more images of a sub-region of the cell samples are recorded in the transmission mode or in the fluorescence mode and at least one or more images of the same sub-region of the cell samples are recorded in the fluorescence mode by at least one microscope camera.

Abstract: The invention relates to a method for providing at least one evaluation method for samples (2) in at least one optical application system (5) of a microscope-based application technology, where the following steps are performed: developing the evaluation method at least by an automated training (130) of an evaluation means (60) for an evaluation (120) of a specific type of sample on the basis of the application technology by an optical training system (4), the training (130) determining a training information (200) which at least partially defines the evaluation method, at least the training information (200) for distributing (140) the evaluation method to the at least one application system (5), wherein the provision takes place as a function of the type of sample and of the application technology.

Abstract: The invention relates to a method for microscopic evaluation (120) of a sample (2), in particular at least one uncolored object or cell sample (2), in an optical detection system (1), where the following steps are performed: providing at least two different detection information (110) about the sample (2), in particular by the detection system (1), performing an evaluation (120) of the detection information (110), in particular by an analysis means (60), on the basis of machine-learned transfer information (200), in order to determine result information (140) about the sample (2), the transfer information (200) being trained for a different detection parameterization of the detection information (110), in which the detection information (110) differs from one another in terms of at least one illumination parameter of the detection system (1), in particular in terms of polarization and/or color coding.

Abstract: A TIRFM-capable microscope including: a light source that generates/emits incoherent excitation light onto an optical path that includes a first projection lens system, a spatial filter, a second projection lens system and an objective. The TIRFM-capable microscope also includes a controller; wherein the first projection lens system projects excitation light onto the spatial filter that filters the excitation light with two-dimensional patterns, the spatial filter lies in a plane conjugate to a back focal plane of the objective which includes an objective lens that directs excitation light onto and receives fluorescent light from the sample, wherein, for a numerical aperture NAObj of the objective and a refractive index nspec of the sample NAObj>nspec, and the controller activates the spatial filter to select/generate various two-dimensional patterns and selects/adjusts the position/shape/size of the pattern such that TIRF illumination of the sample is generated.

Abstract: The invention concerns a method for providing an evaluation means (60) for at least one optical application system (5) of a microscope-based application technology, wherein the following steps are performed, in particular each by an optical training system (4): performing an input detection (101) of at least one sample (2) according to the application technology in order to obtain at least one input record (110) of the sample (2) from the input detection (101), performing a target detection (102) of the sample (2) according to a training technology to obtain at least one target record (112) of the sample (2) from the target detection (102), the training technology being different from the application technology at least in that additional information (115) about the sample (2) is provided, training (130) of the evaluation means (60) at least on the basis of the input recording (110) and the target recording (112), in order to obtain a training information (200) of the evaluation means (60), in that vario

Abstract: The present application relates to a method for the cytometric analysis of multiple cell samples by a microscope for examining multiple cell samples under a microscope, wherein the microscope can be or is operated, selectively and/or alternatingly, in a transmission mode and/or in a fluorescence mode, and wherein at least one cell sample has at least one fluorescence marker. The method includes; moving the cell samples continuously in one plane relative to an optical system of the microscope having at least one microscope camera, wherein, during the movement of the cell samples, at least one or more images of a sub-region of the cell samples are recorded in the transmission mode or in the fluorescence mode and at least one or more images of the same sub-region of the cell samples are recorded in the fluorescence mode by at least one microscope camera.

Abstract: The present application relates to a method for the cytometric analysis of multiple cell samples by a microscope for examining multiple cell samples under a microscope, wherein the microscope can be or is operated, selectively and/or alternatingly, in a transmission mode and/or in a fluorescence mode, and wherein at least one cell sample has at least one fluorescence marker. The method includes; moving the cell samples continuously in one plane relative to an optical system of the microscope having at least one microscope camera, wherein, during the movement of the cell samples, at least one or more images of a sub-region of the cell samples are recorded in the transmission mode or in the fluorescence mode and at least one or more images of the same sub-region of the cell samples are recorded in the fluorescence mode by the at least one microscope camera.

Abstract: A digital imaging system and method for correcting errors in the digital imaging system, the digital imaging system having an image sensor, a readout apparatus for reading out the image sensor, wherein a dark current image is captured using the image sensor and the readout apparatus, and a processor comprising hardware wherein the processor is configured to perform the method comprising: generating a Fourier transform by means of a Fourier transformation on the basis of the image data of the dark current image; storing data which describe the Fourier transform; back-transforming the Fourier transform by subjecting the saved data to Fourier transformation and generating a corrective image from the back-transformed data, and correcting image errors in the digital imaging system in another image captured using the digital imaging system by offsetting image data from the captured image with image data from the corrective image.

Abstract: The present application relates to a method for the cytometric analysis of multiple cell samples by means of a microscope for examining multiple cell samples under a microscope, wherein the microscope can be or is operated, selectively and/or alternatingly, in a transmission mode and/or in a fluorescence mode, and wherein at least one cell sample has at least one fluorescence marker. The method includes; moving the cell samples continuously in one plane relative to an optical system of the microscope having at least one microscope camera, wherein, during the movement of the cell samples, at least one or more images of a sub-region of the cell samples are recorded in the transmission mode or in the fluorescence mode and at least one or more images of the same sub-region of the cell samples are recorded in the fluorescence mode by means of the at least one microscope camera.

Abstract: A method for creating an overall image of an object from a plurality of recorded images that each depict a small area of the object, the method including: dividing the overall image into fields; assigning a part of the plurality of recorded images to each of the fields; and composing the plurality of recorded images into the overall image.

Abstract: A digital imaging system and method for correcting errors in the digital imaging system, the digital imaging system having an image sensor, a readout apparatus for reading out the image sensor, wherein a dark current image is captured using the image sensor and the readout apparatus, and a processor comprising hardware wherein the processor is configured to perform the method comprising: generating a Fourier transform by means of a Fourier transformation on the basis of the image data of the dark current image; storing data which describe the Fourier transform; back-transforming the Fourier transform by subjecting the saved data to Fourier transformation and generating a corrective image from the back-transformed data, and correcting image errors in the digital imaging system in another image captured using the digital imaging system by offsetting image data from the captured image with image data from the corrective image.

Abstract: A device, a method and a system for optically determining particle properties, in particular size and reflectivity. The device includes at least one light source assembly having at least one light source, a polarizer assembly, at least one sample holder—which can be illuminated by the least one light source assembly—for accommodating particle preparations to be investigated, at least one analyzer assembly, and at least one imaging device with at least one color-resolving matrix image sensor. The device is designed to guide light reflected by a particle preparation and having a color-coded polarization to the at least one matrix image sensor.

Abstract: A device, a method and a system for optically determining particle properties, in particular size and reflectivity. The device includes at least one light source assembly having at least one light source, a polarizer assembly, at least one sample holder—which can be illuminated by the least one light source assembly—for accommodating particle preparations to be investigated, at least one analyzer assembly, and at least one imaging device with at least one color-resolving matrix image sensor. The device is designed to guide light reflected by a particle preparation and having a color-coded polarization to the at least one matrix image sensor.

Abstract: The invention provides an incubation device, comprising an incubator chamber (12), a specimen carrier (15), which is designed to accommodate a specimen (14) and position it in the interior of the incubator chamber (12) at a specimen position (P), a heating arrangement (38, 40) with at least one heating element (38, 40), which is arranged inside the incubator chamber (12) and releases heat to the interior of the incubator chamber (12), a humidifier (16) for producing a humidifying fluid, and a passage opening (30) arranged between the humidifier (16) and the interior of the incubator chamber (12), through which the humidifying fluid may pass into the interior of the incubator chamber (12), wherein the interior of the incubator chamber (12) is substantially free of directed fluid flow at least at the specimen position (P).

Abstract: A method for creating an overall image of an object from a plurality of recorded images that each depict a small area of the object, the method including: dividing the overall image into fields; assigning a part of the plurality of recorded images to each of the fields; and composing the plurality of recorded images into the overall image.

Abstract: The invention relates to a method and a device for optically scanning a sample. The basic structure of the device comprises at least one adjustment unit and at least one scanning device. The sample is displaced in relation to the scanning device by means of the adjustment unit impinged upon by a control system, or vice versa. According to the invention, adjustment values for the mechanical compensation of play are incorporated, filed in the control system and taken into consideration during adjustment.

Abstract: The invention relates to a method and to a device for optically scanning a sample (1). The device comprises an adjusting unit (2, 3) and a scanning device (4, 5) which comprises an optical unit (4) and a scanning unit (5). The inventive device also comprises a control arrangement (6) which controls the optical unit (4), the scanning unit (5) and the adjusting unit (2, 3). The sample (1) is displaced in relation to the scanning device (4, 5), or vice-versa, by means of the adjusting unit (2, 3) which is impinged upon by the control arrangement (6). As a result, individual images (7), which are obtained by means of the scanning device (4, 5) are assembled to make at least one global image in the control arrangement (6). According to the invention, the respectively detected individual image (7) is evaluated either partially or totally in relation to the contrast thereof, and the obtained contrast values (K) influence the functionality of the entire arrangement.

Abstract: A device and a method for color data correction during color data transmission from a pickup device to an output device. Raw data of the pickup device are converted to uncorrected interim color values with a smaller number of pixels than the pickup device, and the uncorrected interim color values are then subjected to a color correction calculation and are finally converted into corrected interim color values for the output device.

Abstract: Among the proposals provided is a method for the analysis and classification of objects of interest, for example biological or biochemical objects, on the basis of time-lapse images, for example for use in time-lapse analysis in image-base cytometry. Images of the objects of interest, for example cells, are recorded at different moments in time and these images are subjected to a segmentation process to identify image elements as object representations or sub-object representations of objects or sub-objects of interest of objects of interest. Identified object representations or sub-object representations are then associated with one another in images of the time series and are identified as representations of the same object or sub-object or as the result of an object or sub-object. First features manifesting themselves in individual images are detected and second features manifesting themselves in a plurality of images recorded at different times are detected.

Abstract: A portable storage system. The portable storage system comprises a portable storage device having a flash memory element and a loss-prevention unit. The portable storage system further comprises Master and Slave proximity elements. One of the proximity elements is physically connected with the portable storage device, while the other is physically connected with the loss-prevention unit. The Master proximity element is configured to wirelessly determine the presence of the Slave proximity element within a predefined range.