Patents Assigned to One Cell Systems, Inc.
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Patent number: 7556928Abstract: The invention provides methods of analyzing a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.Type: GrantFiled: August 13, 2004Date of Patent: July 7, 2009Assignee: One Cell Systems, Inc.Inventors: Diana Jespersen, Yevgenya Akselband, Patricia McGrath, Jan Trnovsky, Phillip T. Moen
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Publication number: 20080171329Abstract: The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.Type: ApplicationFiled: October 26, 2007Publication date: July 17, 2008Applicant: Cellay, LLC c/o One Cell Systems, Inc.Inventors: Jan Trnovsky, Patricia McGrath
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Publication number: 20080160498Abstract: The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.Type: ApplicationFiled: October 5, 2007Publication date: July 3, 2008Applicant: Cellay, LLC c/o One Cell Systems, Inc.Inventors: Jan Trnovsky, Patricia McGrath
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Publication number: 20070020617Abstract: The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.Type: ApplicationFiled: April 4, 2006Publication date: January 25, 2007Applicant: Cellay, LLC c/o One Cell Systems, Inc.Inventors: Jan Trnovsky, Patricia McGrath
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Publication number: 20050059049Abstract: The present invention provides nucleic acid hybridization probes having a target-binding region and a labeled hairpin structure at at least one end of the probe. The hairpin-labeled probes include oligonucleotides, dendrimers, and primer-extended nucleic acids. The probes can be used in disclosed methods for detection of target nucleic acids. In addition, the oligonucleotide probes can be used in disclosed methods for primer-extension, including, e.g., random priming and PCR amplification, to produce the primer-extended hairpin-labeled probes. Also disclosed are kits comprising the hairpin-labeled oligonucleotide and dendrimer probes. Further, the present invention provides biomolecules (e.g., peptides, polypeptides, carbohydrates, lipids, and the like) that are labeled via linkage to labeled hairpin structures.Type: ApplicationFiled: July 7, 2004Publication date: March 17, 2005Applicant: One Cell Systems, Inc.Inventors: Phillip Moen, Jan Trnovsky
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Patent number: 6806058Abstract: The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.Type: GrantFiled: May 28, 2002Date of Patent: October 19, 2004Assignee: One Cell Systems, Inc.Inventors: Diana Jesperson, Yevgenya Akselband, Patricia McGrath, Jan Trnovsky, Phillip T. Moen
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Publication number: 20030207260Abstract: The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.Type: ApplicationFiled: May 2, 2003Publication date: November 6, 2003Applicant: Cellay, LLC c/o One Cell Systems, Inc.Inventors: Jan Trnovsky, Patricia McGrath
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Publication number: 20030143642Abstract: The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.Type: ApplicationFiled: May 28, 2002Publication date: July 31, 2003Applicant: One Cell Systems, Inc.Inventors: Diana Jesperson, Yevgenya Akselband, Patricia McGrath, Jan Trnovsky, Phillip T. Moen