Abstract: A bone-regeneration material that contains calcium phosphate particles in a biodegradable fiber containing PLGA by using electrospinning. A PLGA resin is heated in a kneader to soften until the viscosity of the resin becomes 102 to 107 Pa·s. A powder of calcium phosphate fine particles is added and mixed with the softened PLGA resin, while the blade of the kneader rotates. The mixture is kneaded by applying thermal and mechanical energy to the mixture through the continuous rotation of the blade of the kneader in the heated state, and aggregations of the calcium phosphate fine particles are disintegrated to prepare a composite in which the calcium phosphate fine particles are dispersed in the PLGA resin. The composite is dissolved in a solvent to prepare a spinning solution. Electrospinning is performed on the spinning solution to manufacture biodegradable fibers having therein the calcium phosphate fine particles substantially uniformly dispersed.

Abstract: A bone regeneration material has a cotton-wool like structure formed of a plurality of electrospun fibers that contain bound BMP-2 through ?-TCP binding peptide. The electrospun biodegradable fiber contains 25-65 vol % of ?-TCP particles distributed in the fiber such that a portion of the ?-TCP particles is exposed on a surface of the electrospun fiber and the remaining portion of the ?-TCP particles is buried in the fiber. ?-TCP binding peptides that are fused with BMP-2 are bound to the ?-TCP particles so that BMP-2 is tethered to ?-TCP particles on the surface of the fibers. Upon implantation of the bone regeneration material in a bone defect site of a human body, BMP-2 that are tethered to ?-TCP particles on the surface of the bone regeneration material promotes proliferation and differentiation of cells at the bone defect site.

Abstract: A method for producing a cotton-wool like material for bone regeneration using a wet spinning method. 50-80 wt % of calcium salt particles and 50-20 wt % of PDLLGA resin are put into a mixing vessel, dissolved in acetone, and stirred to produce a spinning solution with a resin concentration of 10-20 wt % in which said calcium salt particles are dispersed. The produced spinning solution is filled in a syringe, and the spinning solution filled in the syringe is injected into a collector container filled with poor solvent by extruding the spinning solution from the discharge port of an injection needle having a predetermined diameter. The spinning solution injected into the poor solvent is solidified into fibers by interdiffusion of desorption of organic solvent and penetration of poor solvent in the poor solvent solution. The fibers solidified in the poor solvent are deposited in a floating state in the collector vessel without fiber-to-fiber adhesion and collected in a cotton-wool like shape.

Abstract: A cell culture substrate used for growing mesenchymal stem cells (MSC) while maintaining the differentiation potency of the mesenchymal stem cells includes a nonwoven fabric made of resin fibers spun using an electrospinning method. The nonwoven fabric includes a plurality of resin fibers having outer diameters of 10-50 ?m. The plurality of resin fibers are intertangled in random directions. A mesh structure is formed by the intertangled plurality of resin fibers adhering and joining together at locations where the resin fibers contact one another. The mesh structure forms mesh openings that have a substantially elliptical shape with a diameter of 100-200 ?m and that are surrounded by curved fibers. Innumerable air bubble pores having diameters of 0.1-3 ?m are formed over the entire surface of the fibers making up the nonwoven fabric.

Abstract: Provided is a bone-regeneration material comprising biodegradable fibers and exhibiting antimicrobial properties at an early stage following surgery, A method for producing a bone-regeneration material having antimicrobial properties and comprising biodegradable fibers, wherein the bone-regeneration material is produced by a step in which the biodegradable fibers are immersed in an inositol phosphate solution, then subsequently immersed in a solution containing silver ions, the biodegradable fibers have an outer diameter of 10-100 ?m, contain at least 30 wt % or more of a biodegradable resin and 40 wt % or more of calcium compound particles, and some of the calcium compound particles are exposed on the surface of the biodegradable fibers.

Abstract: Adipose-derived stem cells are isolated and extracted from an adipose tissue without using collagenase. An adipose tissue is covered with a nonwoven fabric sheet having sufficient interfiber space and pressed by an appropriate force. Thus, the adipose tissue infiltrates among fibers of the nonwoven fabric and then comes into contact with fibers surrounding the same. By immersing the nonwoven fabric as such in a culture medium, a number of adipose-derived stem cells are allowed to crawl out from the adipose tissue and then adhered to the fiber surface.

Abstract: A guided bone regeneration material is disclosed. The guided bone regeneration material includes biodegradable fibers produced by an electrospinning method. The biodegradable fibers produced by the method include a silicon-releasing calcium carbonate and a biodegradable polymer. The silicon-releasing calcium carbonate is a composite of siloxane and calcium carbonate of vaterite phase. The biodegradable fibers may be coated with apatite. When the guided bone regeneration material is immersed in a neutral aqueous solution, silicon species ions are eluted from the calcium carbonate. The guided bone regeneration material excels in bone reconstruction ability.

Abstract: A bone-regeneration material that contains calcium phosphate particles in a biodegradable fiber containing PLGA by using electrospinning. A PLGA resin is heated in a kneader to soften until the viscosity of the resin becomes 102 to 107 Pa·s. A powder of calcium phosphate fine particles is added and mixed with the softened PLGA resin, while the blade of the kneader rotates. The mixture is kneaded by applying thermal and mechanical energy to the mixture through the continuous rotation of the blade of the kneader in the heated state, and aggregations of the calcium phosphate fine particles are disintegrated to prepare a composite in which the calcium phosphate fine particles are dispersed in the PLGA resin. The composite is dissolved in a solvent to prepare a spinning solution. Electrospinning is performed on the spinning solution to manufacture biodegradable fibers having therein the calcium phosphate fine particles substantially uniformly dispersed.

Abstract: A bone-regeneration material that contains calcium phosphate particles in biodegradable fibers of PLGA manufactured by electrospinning. A PLGA resin is heated in a kneader until the resin viscosity becomes 102 to 107 Pa·s. A powder of calcium phosphate fine particles is added while the blade is rotated. The mixture is kneaded by continuous rotation of the blade in the heated state to disperse the calcium phosphate fine particles to obtain a composite having calcium phosphate fine particles dispersed in the PLGA resin. The composite is dissolved by a solvent, and the PLGA resin is completely dissolved by agitation for a prescribed duration to prepare a spinning solution in which the calcium phosphate fine particles are dispersed. Electrospinning is performed on the spinning solution to manufacture biodegradable fibers having therein the calcium phosphate fine particles substantially uniformly dispersed.

Abstract: A guided bone regeneration material is disclosed. The guided bone regeneration material includes biodegradable fibers produced by an electrospinning method. The biodegradable fibers produced by the method include a silicon-releasing calcium carbonate and a biodegradable polymer. The silicon-releasing calcium carbonate is a composite of siloxane and calcium carbonate of vaterite phase. The biodegradable fibers may be coated with apatite. When the guided bone regeneration material is immersed in a neutral aqueous solution, silicon species ions are eluted from the calcium carbonate. The guided bone regeneration material excels in bone reconstruction ability.

Abstract: A guided bone regeneration material is disclosed. The guided bone regeneration material includes biodegradable fibers produced by an electrospinning method. The biodegradable fibers produced by the method include a silicon-releasing calcium carbonate and a biodegradable polymer. The silicon-releasing calcium carbonate is a composite of siloxane and calcium carbonate of vaterite phase. The biodegradable fibers may be coated with apatite. When the guided bone regeneration material is immersed in a neutral aqueous solution, silicon species ions are eluted from the calcium carbonate. The guided bone regeneration material excels in bone reconstruction ability.

Abstract: A guided bone regeneration material is disclosed. The guided bone regeneration material includes biodegradable fibers produced by an electrospinning method. The biodegradable fibers produced by the method include a silicon-releasing calcium carbonate and a biodegradable polymer. The silicon-releasing calcium carbonate is a composite of siloxane and calcium carbonate of vaterite phase. The biodegradable fibers may be coated with apatite. When the guided bone regeneration material is immersed in a neutral aqueous solution, silicon species ions are eluted from the calcium carbonate. The guided bone regeneration material excels in bone reconstruction ability.

Abstract: A guided bone regeneration material is disclosed. The guided bone regeneration material includes biodegradable fibers produced by an electrospinning method. The biodegradable fibers produced by the method include a silicon-releasing calcium carbonate and a biodegradable polymer. The silicon-releasing calcium carbonate is a composite of siloxane and calcium carbonate of vaterite phase. The biodegradable fibers may be coated with apatite. When the guided bone regeneration material is immersed in a neutral aqueous solution, silicon species ions are eluted from the calcium carbonate. The guided bone regeneration material excels in bone reconstruction ability.

Abstract: A bone-regeneration material that contains calcium phosphate particles in biodegradable fibers of PLGA manufactured by electrospinning. A PLGA resin is heated in a kneader until the resin viscosity becomes 102 to 107 Pa·s. A powder of calcium phosphate fine particles is added while the blade is rotated. The mixture is kneaded by continuous rotation of the blade in the heated state to disperse the calcium phosphate fine particles to obtain a composite having calcium phosphate fine particles dispersed in the PLGA resin. The composite is dissolved by a solvent, and the PLGA resin is completely dissolved by agitation for a prescribed duration to prepare a spinning solution in which the calcium phosphate fine particles are dispersed. Electrospinning is performed on the spinning solution to manufacture biodegradable fibers having therein the calcium phosphate fine particles substantially uniformly dispersed.

Abstract: Rebuilding a defected bone by activating the innate self-regeneration ability of bone requires a considerably long period of time. The purpose of the present invention is to provide a bone defect filling material that initiates a bone rebuilding activity as quickly as possible after implantation and thereafter remains in the defect to continue promoting bone formation activity until sufficient bone formation has been achieved for the rebuilding of the defect. The present invention provides a cotton-like bone defect filling material comprising biodegradable fibers produced by electrospinning. The biodegradable fibers contain 40-60 wt % of calcium phosphate particles and 10 wt % or more of silicon-releasing calcium carbonate particles, with the remainder containing 30 wt % or more of poly(L-lactic acid) polymer, and the amount of the poly(L-lactic acid) polymer that is non-crystalline is 75-98%.

Abstract: The present invention addresses the problem of providing a drug formulation material with which localized sustained release of a drug at any site in the body is possible, and which has good bioabsorption and is absorbed and broken down by the body after sustained release of the drug. A drug formulation material that has an exceedingly high sustained release effect, and that solves the foregoing problem, was successfully developed by dissolving a biodegradable resin and a drug in a solvent to prepare a spinning solution, and spinning fibers from the spinning solution by electrospinning.

Abstract: A fiber wadding for filling bone defects having a flocculent three-dimensional structure is disclosed. The fiber wadding includes a plurality of fibers that contain a biodegradable resin as a principal component and a siloxane. Outside diameter of the plurality of fibers of the wadding is from about 0.05 ?m to about 30 ?m. Bulk density of the fiber wadding is about 0.005-0.3 g/cm3.

Abstract: A method for producing a flocculent three dimensional fibrous bioactive material using an electrospinning process includes electrospinning a biodegradable fiber by applying an electrical charge to a nozzle of an electrospinning apparatus such that a spinning solution contained therein is made into an electrospun fiber, which is attracted toward a collector container that is electrically grounded and is filled with an ethanol liquid; receiving the electrospun fiber emitted from the nozzle on a surface of the ethanol liquid in the collector container; recovering and drying the electrospun fiber to obtain the flocculent three dimensional fibrous bioactive material having a bulk density of from about 0.01 g/cm3 to about 0.1 g/cm3 as measured in accordance with JIS L 1097.

Abstract: Rebuilding a defected bone by activating the innate self-regeneration ability of bone requires a considerably long period of time. The purpose of the present invention is to provide a bone defect filling material that initiates a bone rebuilding activity as quickly as possible after implantation and thereafter remains in the defect to continue promoting bone formation activity until sufficient bone formation has been achieved for the rebuilding of the defect. The present invention provides a cotton-like bone defect filling material comprising biodegradable fibers produced by electrospinning. The biodegradable fibers contain 40-60 wt % of calcium phosphate particles and 10 wt % or more of silicon-releasing calcium carbonate particles, with the remainder containing 30 wt % or more of poly(L-lactic acid) polymer, and the amount of the poly(L-lactic acid) polymer that is non-crystalline is 75-98%.

Abstract: A fiber wadding for filling bone defects having a flocculent three-dimensional structure is disclosed. The fiber wadding includes a plurality of fibers that contain a biodegradable resin as a principal component and a siloxane. Outside diameter of the plurality of fibers of the wadding is from about 0.05 ?m to about 30 ?m. Bulk density of the fiber wadding is about 0.005-0.3 g/cm3.