Patents Assigned to Oxford Nanopore Technologies PLC
-
Patent number: 11965183Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: GrantFiled: October 13, 2021Date of Patent: April 23, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
-
Publication number: 20240124915Abstract: The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.Type: ApplicationFiled: February 17, 2023Publication date: April 18, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Clive Gavin Brown, Daniel Ryan Garalde, Andrew John Heron, Daniel John Turner, James White
-
Patent number: 11959906Abstract: A time-ordered series of measurements of a polymer made during translocation of the polymer through a Nanopore are analysed. The measurements are dependent on the identity of k-mers in the Nanopore, a k-mer bring k polymer units of the polymer, where k is a positive integer. The method involves deriving, from the series of measurements, a feature vector of time-ordered features representing characteristics of the measurements; and determining similarity between the derived feature vector and at least one other feature vector.Type: GrantFiled: January 11, 2019Date of Patent: April 16, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Stuart William Reid, James Anthony Clarke, James White, Gavin Harper
-
Patent number: 11959135Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.Type: GrantFiled: October 7, 2020Date of Patent: April 16, 2024Assignee: Oxford Nanopore Technologies PLCInventors: James Anthony Clarke, James White, John Milton, Clive Gavin Brown
-
Publication number: 20240117337Abstract: A method of amplifying a target polynucleotide, comprising: providing a template polynucleotide comprising a 5? hairpin, a target polynucleotide and a 3? hairpin, wherein the 5? hairpin comprises one or more non-canonical nucleotides and contacting the template polynucleotide with a polymerase and canonical nucleotides, wherein the polymerase extends, using the canonical nucleotides, the target polynucleotide from its 3? end to form a first extended polynucleotide comprising the 5? hairpin at its 5? end and the complement of the 5? hairpin at its 3? end, wherein the complement of the 5? hairpin forms a 3? hairpin; and the polymerase extends the first extended polynucleotide from its 3? end to form a second extended polynucleotide comprising the 5? hairpin at its 5? end and the complement of the 5? hairpin at its 3? end, wherein the complement of the 5? hairpin forms a 3? hairpin.Type: ApplicationFiled: June 23, 2023Publication date: April 11, 2024Applicant: Oxford Nanopore Technologies PLCInventor: James White
-
Publication number: 20240117421Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.Type: ApplicationFiled: January 26, 2023Publication date: April 11, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Yayne Wallace
-
Publication number: 20240117422Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.Type: ApplicationFiled: May 5, 2023Publication date: April 11, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace
-
Patent number: 11946102Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.Type: GrantFiled: October 7, 2020Date of Patent: April 2, 2024Assignee: Oxford Nanopore Technologies PLCInventors: James Anthony Clarke, James White, John Milton, Clive Gavin Brown
-
Patent number: 11945840Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: GrantFiled: March 18, 2021Date of Patent: April 2, 2024Assignees: VIB VZW, Vrije Universiteit Brussel, Oxford Nanopore Technologies PLCInventors: Han Remaut, Sander Van Der Verren, Nani Van Gerven, Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, John Joseph Kilgour
-
Patent number: 11939359Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.Type: GrantFiled: July 26, 2021Date of Patent: March 26, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, Mark Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
-
Publication number: 20240076719Abstract: A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising: (i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein: (a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and (b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides; (ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore; (iii) measuring current blockades having a duration within a defined window, wherein: (a) the one or more non-natuType: ApplicationFiled: January 27, 2023Publication date: March 7, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Nicholas Antony Smith, Daniel John Turner, Daniel George Fordham, James White
-
Publication number: 20240076729Abstract: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.Type: ApplicationFiled: June 18, 2021Publication date: March 7, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Rebecca Victoria Bowen, Clive Gavin Brown, Mark John Bruce, Daniel Ryan Garalde, James Edward Graham, Andrew John Heron, Etienne Raimondeau, James White, Christopher Peter Youd
-
Patent number: 11920193Abstract: Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.Type: GrantFiled: June 6, 2019Date of Patent: March 5, 2024Assignee: Oxford Nanopore Technologies PLCInventors: James Anthony Clarke, James White, Richard Muscat, Jessica Mary May Johnson, Ramiz Iqbal Nathani, Andrew John Heron, Mark John Bruce, Lakmal Nishantha Jayasinghe, Domenico Caprotti, David Jackson Stoddart, Rebecca Victoria Bowen, Christopher James Wright, Paul Richard Moody
-
Patent number: 11921103Abstract: A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units.Type: GrantFiled: June 21, 2019Date of Patent: March 5, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Stuart William Reid, Gavin Harper, Clive Gavin Brown, James Anthony Clarke, Andrew John Heron
-
Publication number: 20240069007Abstract: Devices for improved nanopore sensing are described. An example device has a structure arranged to separate an analyte reservoir and an outlet chamber. An example device has a structure arranged to separate an analyte reservoir and an outlet chamber. The structure can include an array of nanopore structures, each nanopore structure comprising a passage for fluid connection through the structure between the analyte reservoir and outlet chamber. Control terminals can be arranged for applying a control signal to alter the electrical potential difference across that nanopore structure. Some embodiments include an electronic circuit configured to detect a signal from an electrical transduction element at each nanopore structure. Additional structural features and methods of operating and making the devices are described.Type: ApplicationFiled: September 1, 2023Publication date: February 29, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Ping Xie, Justin Mills, Ken Healy
-
Patent number: 11913936Abstract: An apparatus for supporting an array of layers of amphiphilic molecules, the apparatus comprising: a body, formed in a surface of the body, an array of sensor wells capable of supporting a layer of amphiphilic molecules across the sensor wells, the sensor wells each containing an electrode for connection to an electrical circuit, and formed in the surface of the body between the sensor wells, flow control wells capable of smoothing the flow of a fluid across the surface.Type: GrantFiled: December 20, 2022Date of Patent: February 27, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Jason Robert Hyde, James Anthony Clarke, Gaƫlle Anne-Leonie Andreatta
-
Publication number: 20240060969Abstract: The invention relates to a method of determining the presence or absence of a target analyte in a sample. The method comprises immobilising any target analyte present in the sample on a surface; contacting the surface with: (i) a first detection agent that binds specifically to the target analyte; and (ii) a reporter polynucleotide, wherein the reporter polynucleotide is bound to, or binds to, the first detection agent; and contacting a transmembrane pore with any reporter polynucleotide that has been immobilised on the surface, wherein the reporter polynucleotide is immobilised on the surface by binding of the first agent to the target analyte, and using the transmembrane pore to detect the reporter polynucleotide, thereby determining the presence or absence of the target analyte in the sample.Type: ApplicationFiled: August 24, 2023Publication date: February 22, 2024Applicant: Oxford Nanopore Technologies PLCInventor: Daniel George Fordham
-
Publication number: 20240060126Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein.Type: ApplicationFiled: June 27, 2023Publication date: February 22, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Clive Gavin Brown, James White, Andrew John Heron, Mark John Bruce, Christopher Peter Youd, Rebecca Victoria Bowen
-
Publication number: 20240044865Abstract: Provided herein are methods of characterising a target polypeptide as it moves with respect to a nanopore. Also provided are related kits, systems and apparatuses for carrying out such methods.Type: ApplicationFiled: July 24, 2023Publication date: February 8, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, James Edward Graham, Melania Slawa Strycharska
-
Patent number: 11884950Abstract: The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.Type: GrantFiled: January 7, 2021Date of Patent: January 30, 2024Assignee: Oxford Nanopore Technologies PLCInventors: John Milton, Sobia Nayyar, Jan Riedl, Ryosuke Ogaki