Abstract: Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.
Type:
Grant
Filed:
June 19, 2014
Date of Patent:
November 28, 2017
Assignee:
Phage Diagnostics, Inc.
Inventors:
Michael Sandor Koeris, Julie Thomason, Michael Cappillino, Robert Patrick Shivers, Daniel Robert Brownell, Jayson L. Bowers, Timothy Kuan Ta Lu, Edyta Krzymanska-Olejnik