Abstract: A composition for delivering an agent to a cell, comprising a bispecific affinity reagent and a pH-responsive, membrane destabilizing polymer. The bispecific affinity reagent may include a first affinity reagent covalently linked to a second affinity reagent, wherein the first affinity reagent binds to a molecule on the surface of a cell, and the second affinity reagent binds to an intracellular target.
Type:
Grant
Filed:
May 13, 2009
Date of Patent:
September 2, 2014
Assignees:
University of Washington, PhaseRx, Inc.
Inventors:
Patrick S. Stayton, Allan S. Hoffman, Anthony J. Convertine, Craig L. Duvall, Robert W. Overell, Paul H. Johnson
Abstract: A composition for delivering an agent to a cell, comprising a bispecific affinity reagent and a pH-responsive, membrane destabilizing polymer. The bispecific affinity reagent may include a first affinity reagent covalently linked to a second affinity reagent, wherein the first affinity reagent binds to a molecule on the surface of a cell, and the second affinity reagent binds to an intracellular target.
Type:
Application
Filed:
February 5, 2014
Publication date:
August 14, 2014
Applicants:
PHASERX, INC., UNIVERSITY OF WASHINGTON
Inventors:
Patrick S. Stayton, Allan S. Hoffman, Anthony Convertine, Craig L. Duvall, Robert Overell, Paul Johnson
Abstract: Provided herein are monomers incorporating folate or other targeting agent, polymers prepared therefrom, polymers prepared therefrom having a therapeutic agent covalently coupled thereto, as well as micelles and therapeutic compositions thereof.
Type:
Application
Filed:
November 17, 2010
Publication date:
January 10, 2013
Applicants:
PhaseRx, Inc., University of Washington through its Center for Commercialization
Inventors:
Sean D. Monahan, Paul H. Johnson, Michael S. DeClue, Priyadarsi De, Anna S. Gall, Patrick S. Stayton, Allan S. Hoffman, Charbel Diab
Abstract: Compositions comprising a heterogeneous polymeric micelle and an agent (e.g., a polynucleotide) associated with the micelle are disclosed, together with methods for intracellular delivery of such agent.
Type:
Application
Filed:
May 13, 2009
Publication date:
January 26, 2012
Applicants:
PHASERX, INC., UNIVERSITY OF WASHINGTON
Inventors:
Paul Johnson, Patrick S. Stayton, Allan S. Hoffman, Robert Overell, Anna Gall, Mary Prieve, Amber Paschal, Charbel Diab, Priyadarsi De
Abstract: Polymeric compounds having spatially controlled bioconjugation sites are described. Functionalization is achieved by selective co-terminal chain extension of polymer chains by radical polymerization, such as reversible addition-fragmentation chain transfer (RAFT) polymerization.
Type:
Application
Filed:
December 8, 2009
Publication date:
December 15, 2011
Applicants:
PhaseRx, Inc., University of Washington
Inventors:
Patrick S. Stayton, Allan S. Hoffman, Anthony J. Convertine, Scott M. Henry, Robert W. Overell, Paul H. Johnson
Abstract: Provided herein are multiblock copolymers, as well as micelles and therapeutic compositions thereof.
Type:
Application
Filed:
November 6, 2009
Publication date:
November 24, 2011
Applicants:
PHASERX, INC., UNIVERSITY OF WASHINGTON
Inventors:
Mary G. Prieve, Paul H. Johnson, Patrick S. Stayton, Allan S. Hoffman, Robert W. Overell, Anna S. Gall, Amber E.E. Paschal, Charbel Diab, Priyadarsi De, Michael S. DeClue, Sean D. Monahan
Abstract: A composition for delivering an agent to a cell, comprising a bispecific affinity reagent and a pH-responsive, membrane destabilizing polymer. The bispecific affinity reagent may include a first affinity reagent covalently linked to a second affinity reagent, wherein the first affinity reagent binds to a molecule on the surface of a cell, and the second affinity reagent binds to an intracellular target.
Type:
Application
Filed:
May 13, 2009
Publication date:
November 17, 2011
Applicants:
PHASERX, INC., UNIVERSITY OF WASHINGTON
Inventors:
Patrick S. Stayton, Allan S. Hoffman, Anthony J. Convertine, Craig L. Duvall, Robert W. Overell, Paul H. Johnson
Abstract: Provided herein are micelles comprising a plurality of copolymers. In certain instances, micelles provided herein are pH sensitive particles.
Type:
Application
Filed:
May 13, 2009
Publication date:
November 17, 2011
Applicants:
PHASERX, INC., UNIVERSITY OF WASHINGTON
Inventors:
Paul Johnson, Patrick S. Stayton, Allan S. Hoffman, Robert Overell, Anna Gall, Mary Prieve, Amber Paschal, Charbel Diab, Priyadarsi De
Abstract: Provided herein are polymeric carriers suitable for the delivery of polynucleotides (e.g. oligonucleotides) and/or other therapeutic agents into a living cell.
Type:
Application
Filed:
May 13, 2009
Publication date:
June 16, 2011
Applicants:
UNIVERSITY OF WASHINGTON, PHASERX, INC.
Inventors:
Patrick S. Stayton, Allan S. Hoffman, Anthony J. Convertine, Craig L. Duvall, Danielle Benoit, Robert W. Overell, Paul H. Johnson, Anna S. Gall, Mary G. Prieve, Amber E.E. Paschal, Charbel Diab, Priyadarsi De
Abstract: Composition comprising a polymeric micelle and an associated polynucleotide.
Type:
Application
Filed:
May 13, 2009
Publication date:
June 16, 2011
Applicants:
UNIVERSITY OF WASHINGTON, PHASERX, INC.
Inventors:
Paul H. Johnson, Patrick S. Stayton, Allan S. Hoffman, Anthony J. Convertine, Robert W. Overell, Anna S. Gall, Mary G. Prieve, Amber E.E. Paschal, Charbel Diab, Priyadarsi De
Abstract: Described herein are copolymers, and methods of making and utilizing such copolymers. Such copolymers have at least two blocks: a first block that has at least one unit that is hydrophilic at physiologic pH, and a second block that has hydrophobic groups. This second block further has at least one unit with a group that is anionic at about physiologic pH. The described copolymers are disruptive of a cellular membrane, including an extracellular membrane, an intracellular membrane, a vesicle, an organelle, an endosome, a liposome, or a red blood cell. Preferably, in certain instances, the copolymer disrupts the membrane and enters the intracellular environment. In specific examples, the copolymer is endosomolytic.
Type:
Application
Filed:
May 13, 2009
Publication date:
June 16, 2011
Applicants:
UNIVERSITY OF WASHINGTON, PHASERX, INC.
Inventors:
Patrick S. Stayton, Allan S. Hoffman, Anthony J. Convertine, Danielle Benoit, Craig L. Duvall, Paul H. Johnson, Anna S. Gall
Abstract: Polymer bioconjugate having a RNAi agent covalently coupled to the alpha or omega end of a pH-dependent membrane-destabilizing polymer.
Type:
Application
Filed:
May 13, 2009
Publication date:
June 2, 2011
Applicants:
UNIVERSITY OF WASHINGTON, PHASERX, INC.
Inventors:
Paul H. Johnson, Patrick S. Stayton, Allan S. Hoffman, Anthony J. Convertine, Craig L. Duvall, Danielle Benoit, Chen-Chang Lee, Robert W. Overell, Anna S. Gall, Mary G. Prieve, Amber E.E. Paschal, Charbel Diab, Priyadarsi De
Abstract: Provided herein are micellic assemblies comprising a plurality of copolymers. In certain instances, micellic assemblies provided herein are pH sensitive particles.
Type:
Application
Filed:
May 13, 2009
Publication date:
May 26, 2011
Applicants:
UNIVERSITY OF WASHINGTON, PHASERX, INC.
Inventors:
Patrick S. Stayton, Allan S. Hoffman, Anthony Convertine, Craig L. Duvall, Danielle Benoit, Robert W. Overell, Paul H. Johnson, Anna S. Gall, Mary G. Prieve, Amber E.E. Paschal, Charbel Diab, Priyadarsi De