Abstract: The invention provides improved plant transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method includes various transformation protocols for monocots, such as maize and sorghum, using a combination of media and light conditions to achieve increased efficiency of monocot transformation and increased callus initiation frequencies.
Type:
Application
Filed:
January 26, 2010
Publication date:
July 29, 2010
Applicant:
PIONEER HI-BRED, INC.
Inventors:
XINLI E. WU, MYEONG-JE CHO, ZUO-YU ZHAO
Abstract: Promoter sequences and their essential regions are identified which provide for male tissue-preferred expression. The nucleotide sequences are useful in mediating male fertility in plants.
Type:
Grant
Filed:
August 8, 2005
Date of Patent:
January 8, 2008
Assignee:
Pioneer Hi-Bred, Inc.
Inventors:
Marc C. Albertsen, Tim Fox, Gary Huffman, Mary Trimnell
Abstract: The present invention relates to isolated male tissue-preferred regulatory sequences from Ms45 gene. The invention also relates to the use of Ms45 gene male tissue-preferred regulatory sequences in mediating male fertility in a plant, and in producing hybrid seed. The Ms45 male tissue-preferred regulatory region can be operably linked to exogenous genes encoding cytotoxins or inhibitory molecules. The invention also relates to plant cells, plant tissue and plants that contain the MS45 gene regulatory sequences.
Type:
Grant
Filed:
November 14, 2003
Date of Patent:
December 26, 2006
Assignee:
Pioneer Hi-Bred, Inc.
Inventors:
Marc C. Albertsen, Timothy W. Fox, Carl W. Garnaat, Gary Huffman, Timmy L. Kendall
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