Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Abstract: The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Abstract: Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.

Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.

Abstract: A novel maize variety designated X08R677 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X08R677 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X08R677 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X08R677, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X08R677 are provided. Methods for producing maize varieties derived from maize variety X08R677 and methods of using maize variety X08R677 are disclosed.

Abstract: A novel soybean variety, designated 5PBUG58 is provided. Also provided are the seeds of soybean variety 5PBUG58, cells from soybean variety 5PBUG58, plants of soybean 5PBUG58, and plant parts of soybean variety 5PBUG58. Methods provided include producing a soybean plant by crossing soybean variety 5PBUG58 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PBUG58, methods for producing other soybean varieties or plant parts derived from soybean variety 5PBUG58, and methods of characterizing soybean variety 5PBUG58. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PBUG58 are further provided.

Abstract: A novel canola variety designated G00012 and seed, plants and plant parts thereof. Methods for producing a canola plant that comprise crossing canola variety G00012 with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into G00012 through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby. Hybrid canola seed, plant or plant part produced by crossing the canola variety G00012 or a locus conversion of G00012 with another canola variety.

Abstract: The field is molecular biology, and more specifically, methods for chromosomal engineering of multiple native genes, such as disease resistance genes in a genomic locus using site-specific editing to produce plants. Also described herein are methods of generating heterologous genomic locus in a plant that comprises a plurality of intraspecies polynucleotide sequences.

Abstract: Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.

Abstract: Systems and methods for transfection using a microfluidic device are disclosed. Microdroplets encapsulate cells, transfection molecules, and cationic lipid transfection reagent. Droplet chaotic advection in a rendering channel of the system results in a uniform lipid-DNA complex (lipoplex) formation, which can improve gene delivery efficacy. The shear stress exerted on cell membranes during the chaotic mixing increases membrane permeability, which when combined with the co-confinement of cell and lipoplex, improves transfection efficiency of the cell. The systems and methods can be used for a variety of applications such as gene therapy, in vitro fertilization, regenerative medicine, cancer treatment, and vaccines.

Abstract: The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.

Abstract: A novel maize variety designated 1PDAT19 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1PDAT19 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1PDAT19 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1PDAT19 or a locus conversion of 1PDAT19 with another maize variety.

Abstract: A novel maize variety designated PH424Z and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH424Z with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH424Z through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH424Z or a locus conversion of PH424Z with another maize variety.

Abstract: The present embodiments of the invention generally relate to methods for sorting and imaging insects, including egg and larval life stages, useful for automated high throughput bioassays.

Abstract: Compositions and methods of identifying and characterizing potential gene editing on-target and off-target sites and/or edits in a nucleic acid are provided.

Abstract: A novel maize variety designated X95R017 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X95R017 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X95R017 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X95R017, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X95R017 are provided. Methods for producing maize varieties derived from maize variety X95R017 and methods of using maize variety X95R017 are disclosed.

Abstract: A novel soybean variety, designated 5PCLA69 is provided. Also provided are the seeds of soybean variety 5PCLA69, cells from soybean variety 5PCLA69, plants of soybean 5PCLA69, and plant parts of soybean variety 5PCLA69. Methods provided include producing a soybean plant by crossing soybean variety 5PCLA69 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PCLA69, methods for producing other soybean varieties or plant parts derived from soybean variety 5PCLA69, and methods of characterizing soybean variety 5PCLA69. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PCLA69 are further provided.

Abstract: A novel soybean variety, designated 5PNFE20 is provided. Also provided are the seeds of soybean variety 5PNFE20, cells from soybean variety 5PNFE20, plants of soybean 5PNFE20, and plant parts of soybean variety 5PNFE20. Methods provided include producing a soybean plant by crossing soybean variety 5PNFE20 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PNFE20, methods for producing other soybean varieties or plant parts derived from soybean variety 5PNFE20, and methods of characterizing soybean variety 5PNFE20. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PNFE20 are further provided.