Abstract: A method for the determination of nucleic acids which is highly specific and simple comprises the formation of a triple stranded binding complex including two separate, different probe molecules and detecting the formation of the complex via the inclusion of one of the probes. The method can be used to differentiate between nucleic acids having a single base difference in sequence.
Abstract: Nucleic acid analogs provide a particularly useful tool for the preparation of complex polymeric structures of defined geometry because they are relatively stable to reaction conditions for the preparation of such structures and provide the opportunity to induce reactive groups which would not be possible with usual nucleic acids.
Type:
Grant
Filed:
September 7, 2000
Date of Patent:
February 18, 2003
Assignee:
PNA Diagnostics A/S
Inventors:
Hans-Georg Batz, Troels Koch, Henrik Frydenlund Hansen
Abstract: A nucleic acid analog comprising a polymeric strand which includes a sequence of ligands bound to a backbone made up of linked backbone moieties, which analog is capable of hybridization to a nucleic acid of complementary sequence, further comprising a chelating moiety capable of binding at least one metal ion by chelation.
Type:
Grant
Filed:
May 29, 1998
Date of Patent:
February 12, 2002
Assignee:
PNA Diagnostics A/S
Inventors:
Christopher John Stanley, Henrik Orum, Mikkel Jorgensen
Abstract: An assay procedure to quantify the amount of starting template in a nucleic acid amplification procedure such as PCR involves trying the amplification in the presence of varying amounts of a nucleic acid analogue which in sufficient quantity will inhibit said amplification procedure by selective interaction with a nucleic acid species present, e.g. a PNA as described in WO-02/20703. The amount of template is given by the amount of nucleic acid analogue critical to cause failure of said amplification procedure.
Abstract: A selected region of a nucleic acid is protected from attach by nuclease by completing to the nucleic acid a nucleic acid analogue of the PNA type. The surviving sequence may be detected in an assay, optionally after amplification. A PCR reaction may be sterilized and its product assayed by protecting a characterizing region of the product by PNA hybridization followed by nuclease degradation of unprotected nucleic acid.
Type:
Grant
Filed:
June 6, 1996
Date of Patent:
January 19, 1999
Assignee:
PNA Diagnostics A/S
Inventors:
Christopher John Stanley, Henrik Orum, Mikkell Jorgensen, Ole Basboll
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