Abstract: Provided herein are media, methods, kits, primers and oligonucleotide probes for use in the molecular detection of pathogens. These may be used in combination for the rapid, high-throughput screening PCR-based techniques to simultaneously detect multiple pathogens. The multiplex-detection methods have improved sensitivity and specificity for the detection of multiple pathogens simultaneously. Real-time PCR assaying techniques using such primers include microarrays and multiplex arrays, the latter optionally simultaneously with oligonucleotide TaqMan probes.
Type:
Grant
Filed:
April 6, 2016
Date of Patent:
April 23, 2024
Assignee:
POLYSKOPE LABS
Inventors:
Cyrus Cody Zegrati, Michael Benjamin Centola, Bobby Alfonso Gramling, Jr., Paul Simon Smith
Abstract: Provided herein are media, methods, kits, primers and oligonucleotide probes for use in the molecular detection of pathogens. These may be used in combination for the rapid, high-throughput screening PCR-based techniques to simultaneously detect multiple pathogens. The multiplex-detection methods have improved sensitivity and specificity for the detection of multiple pathogens simultaneously. Real-time PCR assaying techniques using such primers include microarrays and multiplex arrays, the latter optionally simultaneously with oligonucleotide TaqMan probes.
Type:
Application
Filed:
March 13, 2020
Publication date:
July 6, 2023
Applicant:
POLYSKOPE LABS
Inventors:
Cyrus Cody ZEGRATI, Michael Benjamin CENTOLA, Paul Simon SMITH