Abstract: The present disclosure describes reproducible methods for the Agrobacterium-mediated production of stable, genetically transformed, fertile tarwi plants (Lupinus mutabilis Sweet) having seed-specific expression of human adenosine deaminase enzyme (hADA) or a functional variant thereof. The method involves slicing a tarwi seed embryo to produce an explant; infecting the explant in co-cultivation medium containing an agrobacterium having a polynucleotide sequence encoding hADA or variant; thereby generating a transformed explant; elongating a transformed shoot from the transformed explant; and regenerating a transformed tarwi plant from the elongated shoot. Seeds and plants so formed are also described herein. Further, methods for the recovery and purification of recombinant hADA, or functional variant from a transformed tarwi plant are described.

Abstract: The present disclosure describes reproducible methods for the Agrobacterium-mediated production of stable, genetically transformed, fertile tarwi plants (Lupinus mutabilis Sweet) having seed-specific expression of human adenosine deaminase enzyme (hADA) or a functional variant thereof. The method involves slicing a tarwi seed embryo to produce an explant; infecting the explant in co-cultivation medium containing an agrobacterium having a polynucleotide sequence encoding hADA or variant; thereby generating a transformed explant; elongating a transformed shoot from the transformed explant; and regenerating a transformed tarwi plant from the elongated shoot. Seeds and plants so formed are also described herein. Further, methods for the recovery and purification of recombinant hADA, or functional variant from a transformed tarwi plant are described.

Abstract: A method for the just-in-time delivery of harvested plant material and plant compounds grown in a genetically-isolated growth environment is provided. The desired crop is grown in an underground growth chamber according to a predetermined growing model in which all growing conditions are controlled. Component yield and completion time can be accurately forecast such that the harvest time of the plant material, or the completion of upstream plant extract recovery, can be accurately forecast. The use of an underground growth chamber allows for complete control over environmental factors in growth of the plant material, and also allows for the best possible protection of the growing environment against any contamination of genetic material from outside of the chamber, as well as protection of the outdoor environment against any contamination of genetic material from inside of the chamber. The method will be particularly useful in the production of plant-made pharmaceuticals and nutraceutical.

Abstract: A method for the just-in-time delivery of harvested plant material and plant compounds grown in a genetically-isolated growth environment is provided. The desired crop is grown in an underground growth chamber according to a predetermined growing model in which all growing conditions are controlled. Component yield and completion time can be accurately forecast such that the harvest time of the plant material, or the completion of upstream plant extract recovery, can be accurately forecast. The use of an underground growth chamber allows for complete control over environmental factors in growth of the plant material, and also allows for the best possible protection of the growing environment against any contamination of genetic material from outside of the chamber, as well as protection of the outdoor environment against any contamination of genetic material from inside of the chamber. The method will be particularly useful in the production of plant-made pharmaceuticals and nutraceutical.

Abstract: A method for the just-in-time delivery of harvested plant material and plant compounds grown in a genetically-isolated growth environment is provided. The desired crop is grown in an underground growth chamber according to a predetermined growing model in which all growing conditions are controlled. Component yield and completion time can be accurately forecast such that the harvest time of the plant material, or the completion of upstream plant extract recovery, can be accurately forecast. The use of an underground growth chamber allows for complete control over environmental factors in growth of the plant material, and also allows for the best possible protection of the growing environment against any contamination of genetic material from outside of the chamber, as well as protection of the outdoor environment against any contamination of genetic material from inside of the chamber. The method will be particularly useful in the production of plant-made pharmaceuticals and nutraceutical.

Abstract: A method for the just-in-time delivery of harvested plant material and plant compounds grown in a genetically-isolated growth environment is provided. The desired crop is grown in an underground growth chamber according to a predetermined growing model in which all growing conditions are controlled. Component yield and completion time can be accurately forecast such that the harvest time of the plant material, or the completion of upstream plant extract recovery, can be accurately forecast. The use of an underground growth chamber allows for complete control over environmental factors in growth of the plant material, and also allows for the best possible protection of the growing environment against any contamination of genetic material from outside of the chamber, as well as protection of the outdoor environment against any contamination of genetic material from inside of the chamber. The method will be particularly useful in the production of plant-made pharmaceuticals and nutraceutical.