Patents Assigned to Probex
  • Patent number: 9540678
    Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.
    Type: Grant
    Filed: January 31, 2014
    Date of Patent: January 10, 2017
    Assignees: The University of Tokyo, Toyo Boseki Kabushiki Kaisha, ProbeX Inc.
    Inventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
  • Publication number: 20140141416
    Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.
    Type: Application
    Filed: January 31, 2014
    Publication date: May 22, 2014
    Applicants: The University of Tokyo, Toyo Boseki Kabushiki Kaisha, ProbeX Inc.
    Inventors: Takeaki OZAWA, Naomi MISAWA, Kenji MIURA, Tasuku OKAMOTO, Shigeaki NISHII, Kenji MASUDA
  • Publication number: 20130323814
    Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.
    Type: Application
    Filed: May 31, 2013
    Publication date: December 5, 2013
    Applicants: The University of Tokyo, Toyo Boseki Kabushiki Kaisha, ProbeX Inc.
    Inventors: The University of Tokyo, ProbeX Inc., Toyo Boseki Kabushiki Kaisha
  • Patent number: 8470974
    Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.
    Type: Grant
    Filed: May 28, 2010
    Date of Patent: June 25, 2013
    Assignees: The University of Tokyo, ProbeX Inc., Tokyo Boseki Kabushiki Kaisha
    Inventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
  • Publication number: 20120190824
    Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.
    Type: Application
    Filed: May 28, 2010
    Publication date: July 26, 2012
    Applicants: THE UNIVERSITY OF TOKYO, TOYO BOSEKI KABUSHIKI KAISHA, PROBEX INC.
    Inventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
  • Patent number: 6117309
    Abstract: A process for recovering a base oil of lubricating viscosity from used oil in which, following optional pretreatment, used oil is re-refined by distilling it in distillation apparatus having multiple theoretical plates. Impurities are then extracted from the lube range distillate fraction or fractions with a liquid extractant such as N-Methyl-2-Pyrrolidone (NMP) at a temperature below the temperature, if any, of complete miscibility of the extractant and the oil. The oil and extractant are then separated whereupon the extractant is re-used in the process and the oil is subject to further treatment, as necessary, for targeted uses.
    Type: Grant
    Filed: September 8, 1997
    Date of Patent: September 12, 2000
    Assignee: Probex Corporation
    Inventors: Alexander D. B. Daspit, Martin MacDonald, Thomas G. Murray
  • Patent number: 6106699
    Abstract: The invention is directed to a process of reducing the chlorine content of oil as well as its propensity to foul during subsequent distillation processes. The process heats the oil to a temperature in the range of 400 to 700 F and contacts the oil with a non-oxidizing gas, such as steam, for a residence time of at least several minutes. Thereafter the oil may be distilled and/or finished to a base lubricating oil or other distillate product. The oil subject to the process may be used oil, and can be subject to a dewatering or removal of light contaminants treatment prior to being subject to the process.
    Type: Grant
    Filed: April 28, 1998
    Date of Patent: August 22, 2000
    Assignee: Probex
    Inventors: Martin R. MacDonald, Alexander D.B. Daspit, Thomas G. Murray
  • Patent number: 4508686
    Abstract: Apparatus and test film strip for evaluation of a film processor, particularly an x-ray film processor, based on a photodetector signal sequentially indicating the optical density of graded density test areas on a developed film and comparing the output thereof to a preselected voltage relating to the acceptable/too dark threshold of an unexposed or base fog area, the acceptable or too light threshold of a maximum density or dark area and the acceptable/too light and acceptable/too dark threshold of a medium density test area. Sequence testing of the graded density areas is functionally related on a single film strip to timing marks, adapted to be read by photodetector and timing circuitry, the timing marks and graded density test areas being linearly spaced and relatively disposed along the length of film strip.
    Type: Grant
    Filed: May 19, 1982
    Date of Patent: April 2, 1985
    Assignee: Probex, Inc.
    Inventors: Gary S. Shaber, Charles W. Buenzli, Jr.
  • Patent number: 4365895
    Abstract: Apparatus and method for evaluation of a film processor, particularly an x-ray film processor, based on a photodetector signal sequentially indicating the optical density of graded density test areas on a developed film and comparing the output thereof to a preselected voltage relating to the acceptable/too dark threshold of an unexposed or base fog area, the acceptable or too light threshold of a maximum density or dark area and the acceptable/too light and acceptable/too dark threshold of a medium density test area. Sequence testing of the graded density areas is functionally related on a single film strip to timing marks, adapted to be read by photodetector and timing circuitry, the timing marks and graded density test areas being linearly spaced and relatively disposed along the length of film strip.
    Type: Grant
    Filed: December 3, 1980
    Date of Patent: December 28, 1982
    Assignee: Probex, Inc.
    Inventors: Gary S. Shaber, Charles W. Buenzli, Jr.