Patents Assigned to Probex, Inc.
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Patent number: 9540678Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: GrantFiled: January 31, 2014Date of Patent: January 10, 2017Assignees: The University of Tokyo, Toyo Boseki Kabushiki Kaisha, ProbeX Inc.Inventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
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Publication number: 20140141416Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: ApplicationFiled: January 31, 2014Publication date: May 22, 2014Applicants: The University of Tokyo, Toyo Boseki Kabushiki Kaisha, ProbeX Inc.Inventors: Takeaki OZAWA, Naomi MISAWA, Kenji MIURA, Tasuku OKAMOTO, Shigeaki NISHII, Kenji MASUDA
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Publication number: 20130323814Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: ApplicationFiled: May 31, 2013Publication date: December 5, 2013Applicants: The University of Tokyo, Toyo Boseki Kabushiki Kaisha, ProbeX Inc.Inventors: The University of Tokyo, ProbeX Inc., Toyo Boseki Kabushiki Kaisha
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Patent number: 8470974Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: GrantFiled: May 28, 2010Date of Patent: June 25, 2013Assignees: The University of Tokyo, ProbeX Inc., Tokyo Boseki Kabushiki KaishaInventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
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Publication number: 20120190824Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: ApplicationFiled: May 28, 2010Publication date: July 26, 2012Applicants: THE UNIVERSITY OF TOKYO, TOYO BOSEKI KABUSHIKI KAISHA, PROBEX INC.Inventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
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Patent number: 4508686Abstract: Apparatus and test film strip for evaluation of a film processor, particularly an x-ray film processor, based on a photodetector signal sequentially indicating the optical density of graded density test areas on a developed film and comparing the output thereof to a preselected voltage relating to the acceptable/too dark threshold of an unexposed or base fog area, the acceptable or too light threshold of a maximum density or dark area and the acceptable/too light and acceptable/too dark threshold of a medium density test area. Sequence testing of the graded density areas is functionally related on a single film strip to timing marks, adapted to be read by photodetector and timing circuitry, the timing marks and graded density test areas being linearly spaced and relatively disposed along the length of film strip.Type: GrantFiled: May 19, 1982Date of Patent: April 2, 1985Assignee: Probex, Inc.Inventors: Gary S. Shaber, Charles W. Buenzli, Jr.
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Patent number: 4365895Abstract: Apparatus and method for evaluation of a film processor, particularly an x-ray film processor, based on a photodetector signal sequentially indicating the optical density of graded density test areas on a developed film and comparing the output thereof to a preselected voltage relating to the acceptable/too dark threshold of an unexposed or base fog area, the acceptable or too light threshold of a maximum density or dark area and the acceptable/too light and acceptable/too dark threshold of a medium density test area. Sequence testing of the graded density areas is functionally related on a single film strip to timing marks, adapted to be read by photodetector and timing circuitry, the timing marks and graded density test areas being linearly spaced and relatively disposed along the length of film strip.Type: GrantFiled: December 3, 1980Date of Patent: December 28, 1982Assignee: Probex, Inc.Inventors: Gary S. Shaber, Charles W. Buenzli, Jr.