Patents Assigned to Pyrosequencing AB
  • Allele specific primer extension
    Publication number: 20030104372
    Abstract: The present invention provides methods of allele-specific primer extension useful for detecting mutations and genetic variations.
    Type: Application
    Filed: February 23, 2001
    Publication date: June 5, 2003
    Applicant: PYROSEQUENCING AB.
    Inventors: Afshin Ahmadian, Joakim Lundeberg, Pal Nyren
  • Method of sequencing DNA based on the detection of the release of pyrophosphate and enzymatic nucleotide degradation
    Patent number: 6258568
    Abstract: The present invention relates to a method of sequencing DNA, based on the detection of base incorporation by the release of pyrophosphate (PPi) and simultaneous enzymatic nucleotide degradation.
    Type: Grant
    Filed: July 23, 1999
    Date of Patent: July 10, 2001
    Assignee: Pyrosequencing AB
    Inventor: Pal Nyren
  • Method of sequencing DNA
    Patent number: 6210891
    Abstract: The present invention provides a method of identifying a base at a target position in a single-stranded sample DNA sequence wherein an extension primer, which hybridizes to the sample DNA immediately adjacent to the target position, is provided and the sample DNA and extension primer are subjected to a polymerization reaction in the presence of a deoxynucleotide or dideoxynucleotide, whereby the deoxynucleotide or dideoxynucleotide will only become incorporated and release pyrophosphate if it is complementary to the base in the target position. Release of pyrophosphate is detected enzymatically and pyrophosphate detection enzyme(s) are included in the polymerization step.
    Type: Grant
    Filed: July 6, 1999
    Date of Patent: April 3, 2001
    Assignee: Pyrosequencing AB
    Inventors: Pål Nyren, Mathias Uhlen, Mostafa Ronaghi
  • Methods for assessing cardiovascular status and compositions for use thereof
    Patent number: 6197505
    Abstract: The present invention provides methods for assessing cardiovascular status in an individual, which comprise determining the sequence at one or more polymorphic positions within the human genes encoding angiotensin converting enzyme (ACE), angiotensinogen (AGT), and/or type 1 angiotensin II receptor (AT1). The invention also provides isolated nucleic acids encoding ACE, AGT, and AT1 polymorphisms, nucleic acid probes that hybridize to polymorphic positions, kits for the prediction of cardiovascular status, and nucleic acid and peptide targets for use in identifying candidate cardiovascular drugs.
    Type: Grant
    Filed: March 27, 1998
    Date of Patent: March 6, 2001
    Assignee: Pyrosequencing AB
    Inventors: Leif Torbjörn Norberg, Maria Kristina Andersson, Per Harry Rutger Lindström