Abstract: A chemical composition including a fluorescent polymer and a receptor that is specific for both a target biological agent and a chemical moiety including (a) a recognition element, (b) a tethering element, and (c) a property-altering element is disclosed. Both the fluorescent polymer and the receptor are co-located on a support. When the chemical moiety is bound to the receptor, the property-altering element is sufficiently close to the fluorescent polymer to alter the fluorescence emitted by the polymer. When an analyte sample is introduced, the target biological agent, if present, binds to the receptor, thereby displacing the chemical moiety from the receptor, resulting in an increase of detected fluorescence. Assays for detecting the presence of a target biological agent are also disclosed.
Type:
Application
Filed:
September 19, 2006
Publication date:
September 4, 2008
Applicant:
QTL BIOSYSTEMS, LLC
Inventors:
Robert M. JONES, Sriram KUMARASWAMY, Liangde LU, Frauke RININSLAND, Kevin LEY, Wensheng XIA, Duncan MCBRANCH, David G. WHITTEN
Abstract: A chemical composition including a fluorescent polymer and a receptor that is specific for both a target biological agent and a chemical moiety including (a) a recognition element, (b) a tethering element, and (c) a property-altering element is disclosed. Both the fluorescent polymer and the receptor are co-located on a support. When the chemical moiety is bound to the receptor, the property-altering element is sufficiently close to the fluorescent polymer to alter the fluorescence emitted by the polymer. When an analyte sample is introduced, the target biological agent, if present, binds to the receptor, thereby displacing the chemical moiety from the receptor, resulting in an increase of detected fluorescence. Assays for detecting the presence of a target biological agent are also disclosed.
Type:
Grant
Filed:
March 18, 2002
Date of Patent:
October 17, 2006
Assignee:
QTL Biosystems, LLC
Inventors:
Robert M. Jones, Sriram Kumaraswamy, Liangde Lu, Frauke Rininsland, Kevin Ley, Wensheng Xia, Duncan McBranch, David G. Whitten
Abstract: A chemical composition including a moiety comprising a quencher (Q), a tethering element (T), and a ligand (L) that associates with and quenches a fluorescent polymer is disclosed. When an analyte sample is introduced, the ligand (L) binds to a target biological agent if it is present, thereby causing the QTL molecule to separate from the fluorescent polymer resulting in an increase in detected fluorescence. The same chemistry is advantageously employed in a competitive assay. An electric field can also be applied to separate the QTL molecule from the fluorescent polymer. A method for detecting targets for these methods are also disclosed.
Type:
Grant
Filed:
May 8, 2001
Date of Patent:
June 1, 2004
Assignee:
QTL Biosystems LLC
Inventors:
David G. Whitten, Duncan W. McBranch, Robert Jones, Troy S. Bergstedt